Regulation of Sulfate Uptake and Expression of Sulfate Transporter Genes in Brassica oleracea as Affected by Atmospheric H2S and Pedospheric Sulfate Nutrition
| العنوان: | Regulation of Sulfate Uptake and Expression of Sulfate Transporter Genes in Brassica oleracea as Affected by Atmospheric H2S and Pedospheric Sulfate Nutrition |
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| المؤلفون: | Markus Wirtz, Peter Buchner, S. Westerman, Rüdiger Hell, Luit J. De Kok, Malcolm J. Hawkesford, C. Elisabeth E. Stuiver |
| المساهمون: | De Kok lab |
| المصدر: | Plant Physiology, 136(2), 3396-3408. AMER SOC PLANT BIOLOGISTS |
| بيانات النشر: | AMER SOC PLANT BIOLOGISTS, 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | Nitrogen, Physiology, ASSIMILATION, Molecular Sequence Data, Brassica, CURLY KALE, Biological Transport, Active, chemistry.chemical_element, Plant Science, Plant Roots, CLONING, Soil, chemistry.chemical_compound, Gene Expression Regulation, Plant, Gene expression, PLANT ARABIDOPSIS-THALIANA, Genetics, GLUTATHIONE, Hydrogen Sulfide, Sulfate, ATP SULFURYLASE, Phylogeny, ROOTS, Regulation of gene expression, biology, Atmosphere, Sulfates, food and beverages, Transporter, Metabolism, biology.organism_classification, CDNA, Sulfur, chemistry, Biochemistry, Brassica oleracea, SULFUR DEFICIENCY, CYSTEINE BIOSYNTHESIS, Carrier Proteins, Plant Shoots, Signal Transduction, Research Article |
| الوصف: | Demand-driven signaling will contribute to regulation of sulfur acquisition and distribution within the plant. To investigate the regulatory mechanisms pedospheric sulfate and atmospheric H2S supply were manipulated in Brassica oleracea. Sulfate deprivation of B. oleracea seedlings induced a rapid increase of the sulfate uptake capacity by the roots, accompanied by an increased expression of genes encoding specific sulfate transporters in roots and other plant parts. More prolonged sulfate deprivation resulted in an altered shoot-root partitioning of biomass in favor of the root. B. oleracea was able to utilize atmospheric H2S as S-source; however, root proliferation and increased sulfate transporter expression occurred as in S-deficient plants. It was evident that in B. oleracea there was a poor shoot to root signaling for the regulation of sulfate uptake and expression of the sulfate transporters. cDNAs corresponding to 12 different sulfate transporter genes representing the complete gene family were isolated from Brassica napus and B. oleracea species. The sequence analysis classified the Brassica sulfate transporter genes into four different groups. The expression of the different sulfate transporters showed a complex pattern of tissue specificity and regulation by sulfur nutritional status. The sulfate transporter genes of Groups 1, 2, and 4 were induced or up-regulated under sulfate deprivation, although the expression of Group 3 sulfate transporters was not affected by the sulfate status. The significance of sulfate, thiols, and O-acetylserine as possible signal compounds in the regulation of the sulfate uptake and expression of the transporter genes is evaluated. |
| اللغة: | English |
| تدمد: | 1532-2548 0032-0889 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a7cecf35e87d2025f1e49353bcb3e19Test https://doi.org/10.1104/pp.104.046441Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3a7cecf35e87d2025f1e49353bcb3e19 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15322548 00320889 |
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