Spanish multicenter study of the epidemiology and mechanisms of amoxicillin-clavulanate resistance in Escherichia coli
| العنوان: | Spanish multicenter study of the epidemiology and mechanisms of amoxicillin-clavulanate resistance in Escherichia coli |
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| المؤلفون: | Ortega A., Oteo J., Aranzamendi-Zaldumbide M., Bartolomé R.M., Bou G., Cercenado E., Conejo M.C., González-López J.J., Marín M., Martínez-Martínez L., Merino M., Navarro F., Oliver A., Pascual Á., Rivera A., Rodríguez-Baño J., Weber I., Aracil B., Campos J. |
| المصدر: | ANTIMICROBIAL AGENTS AND CHEMOTHERAPY r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau instname |
| بيانات النشر: | AMER SOC MICROBIOLOGY, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | antibiotic resistance, sequence analysis, polymerase chain reaction, prevalence, gene sequence, pulsed field gel electrophoresis, beta lactamase AmpC, minimum inhibitory concentration, Amoxicillin-Potassium Clavulanate Combination, bacterial protein, molecular epidemiology, beta lactamase, beta lactamase TEM 34, beta lactamase TEM 33, beta lactamase TEM 32, beta lactamase TEM 54, beta lactamase TEM 76, beta lactamase TEM 1, beta lactamase TEM 185, genetic variability, Drug Resistance, Bacterial, beta lactamase TEM 35, beta lactamase TEM 79, Escherichia coli, serotype, Prospective Studies, hospital, beta lactamase TEM 30, amoxicillin plus clavulanic acid, Escherichia coli Infections, nonhuman, bacterium isolate, article, nucleotide sequence, penicillinase, unclassified drug, clonal variation, priority journal, Spain, protein oxa 1, community, beta lactamase TEM 40, prospective study |
| الوصف: | We conducted a prospective multicenter study in Spain to characterize the mechanisms of resistance to amoxicillin-clavulanate (AMC) in Escherichia coli. Up to 44 AMC-resistant E. coli isolates (MIC =32/16 µg/ml) were collected at each of the seven participant hospitals. Resistance mechanisms were characterized by PCR and sequencing. Molecular epidemiology was studied by pulsed-field gel electrophoresis (PFGE) and by multilocus sequence typing. Overall AMC resistance was 9.3%. The resistance mechanisms detected in the 257 AMC-resistant isolates were OXA-1 production (26.1%), hyperproduction of penicillinase (22.6%), production of plasmidic AmpC (19.5%), hyperproduction of chromosomic AmpC (18.3%), and production of inhibitor-resistant TEM (IRT) (17.5%). The IRTs identified were TEM-40 (33.3%), TEM-30 (28.9%), TEM-33 (11.1%), TEM-32 (4.4%), TEM-34 (4.4%), TEM-35 (2.2%), TEM-54 (2.2%), TEM-76 (2.2%), TEM-79 (2.2%), and the new TEM-185 (8.8%). By PFGE, a high degree of genetic diversity was observed although two well-defined clusters were detected in the OXA-1-producing isolates: the C1 cluster consisting of 19 phylogroup A/sequence type 88 [ST88] isolates and the C2 cluster consisting of 19 phylogroup B2/ST131 isolates (16 of them producing CTX-M-15). Each of the clusters was detected in six different hospitals. In total, 21.8% of the isolates were serotype O25b/phylogroup B2 (O25b/B2). AMC resistance in E. coli is widespread in Spain at the hospital and community levels. A high prevalence of OXA-1 was found. Although resistant isolates were genetically diverse, clonality was linked to OXA-1-producing isolates of the STs 88 and 131. Dissemination of IRTs was frequent, and the epidemic O25b/B2/ST131 clone carried many different mechanisms of AMC resistance. Copyright © 2012, American Society for Microbiology. All Rights Reserved. |
| تدمد: | 0066-4804 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=RECOLECTA___::2425e7fe44210f5bfb7f068382664afbTest https://www.scopus.com/inward/record.uri?eid=2-s2.0-84862575125&doi=10.1128/AAC.06393-11&partnerID=40&md5=39b275f90a87d7e0794a7bc8aebac749Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.RECOLECTA.....2425e7fe44210f5bfb7f068382664afb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00664804 |
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