Unidirectional steady state rates of central metabolism enzymes measured simultaneously in a living plant tissue
| العنوان: | Unidirectional steady state rates of central metabolism enzymes measured simultaneously in a living plant tissue |
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| المؤلفون: | Lyndon Emsley, Claude Roby, Albrecht Roscher, Philippe Raymond |
| المساهمون: | Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Université Joseph Fourier - Grenoble 1 (UJF), Stéréochimie et Interactions Moléculaires (STIM), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Station de physiologie végétale, Institut National de la Recherche Agronomique (INRA), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL) |
| المصدر: | Journal of Biological Chemistry Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 1998, 273 (39), pp.25053-25061. ⟨10.1074/jbc.273.39.25053⟩ Journal of Biological Chemistry 39 (273), 25053-25061. (1998) Journal of Biological Chemistry, 1998, 273 (39), pp.25053-25061. ⟨10.1074/jbc.273.39.25053⟩ |
| بيانات النشر: | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| مصطلحات موضوعية: | 0106 biological sciences, Uridine Diphosphate Glucose, Magnetic Resonance Spectroscopy, UTP-Glucose-1-Phosphate Uridylyltransferase, plante céréaliere, maïs, Enolase, Isomerase, 01 natural sciences, Biochemistry, Pyrophosphate, zea mays, 03 medical and health sciences, chemistry.chemical_compound, Adenosine Triphosphate, Glycolysis, Hexosephosphates, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Molecular Biology, métabolisme, flux métabolique, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, ATP synthase, biology, Chemistry, Hydrolysis, Phosphotransferases, hypoxie, Cell Biology, Metabolism, RMN, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], Kinetics, Enzyme, biology.protein, Phosphoglucomutase, Energy Metabolism, 010606 plant biology & botany |
| الوصف: | The unidirectional steady state reaction rates of several enzymes and metabolic fluxes of distinct processes were measured simultaneously in hypoxic maize root tips using two-dimensional phosphorus NMR exchange spectroscopy. A single spectrum monitors ATP synthesis and hydrolysis as well as the activities of four enzymes involved in key pathways of central metabolism: UDP-glucose pyrophosphorylase, phosphoglucomutase, hexose-phosphate isomerase, and enolase. The corresponding unidirectional reaction rates and net metabolic fluxes were calculated from spectral intensities. This method provides a unique picture, at enzyme resolution, of how metabolism reacts in a concerted fashion to changes in external parameters such as temperature and oxygen concentration. By increasing hypoxia via an increase in temperature, we measured the expected increase in glycolysis through enolase activity while total ATP synthesis settled. At the same time, we observed a net flux through phosphoglucomutase and UDP-glucose pyrophosphorylase toward carbohydrate synthesis. This result is discussed in relation to the current hypothesis on the turnover of cell walls and sucrose. This reaction also produces a net flux of pyrophosphate, which is needed by pyrophosphate:fructose-6-phosphate 1-phosphotransferase to work as a glycolytic enzyme. |
| وصف الملف: | application/pdf |
| تدمد: | 0021-9258 1083-351X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e6f85b1dbd413eae6a0623dd5c068e75Test https://infoscience.epfl.ch/record/204477Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e6f85b1dbd413eae6a0623dd5c068e75 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00219258 1083351X |
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