دورية أكاديمية
Microbiota- and Radiotherapy-Induced Gastrointestinal Side-Effects (MARS) Study: A Large Pilot Study of the Microbiome in Acute and Late-Radiation Enteropathy.
| العنوان: | Microbiota- and Radiotherapy-Induced Gastrointestinal Side-Effects (MARS) Study: A Large Pilot Study of the Microbiome in Acute and Late-Radiation Enteropathy. |
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| المؤلفون: | Reis Ferreira, M, Andreyev, HJN, Mohammed, K, Truelove, L, Gowan, SM, Li, J, Gulliford, SL, Marchesi, JR, Dearnaley, DP |
| المساهمون: | Reis Ferreira, Jose, Gowan, Sharon, Dearnaley, David |
| بيانات النشر: | AMER ASSOC CANCER RESEARCH |
| سنة النشر: | 2019 |
| المجموعة: | The Institute of Cancer Research (ICR): Publications Repository |
| مصطلحات موضوعية: | Gastrointestinal Tract, Intestinal Mucosa, Feces, Humans, Bacteria, Pelvic Neoplasms, Radiation Injuries, RNA, Ribosomal, 16S, Aged, Middle Aged, Female, Male, Gastrointestinal Microbiome, Radiation Exposure |
| الوصف: | PURPOSE: Radiotherapy is important in managing pelvic cancers. However, radiation enteropathy may occur and can be dose limiting. The gut microbiota may contribute to the pathogenesis of radiation enteropathy. We hypothesized that the microbiome differs between patients with and without radiation enteropathy.Experimental Design: Three cohorts of patients (n = 134) were recruited. The early cohort (n = 32) was followed sequentially up to 12 months post-radiotherapy to assess early radiation enteropathy. Linear mixed models were used to assess microbiota dynamics. The late cohort (n = 87) was assessed cross-sectionally to assess late radiation enteropathy. The colonoscopy cohort compared the intestinal mucosa microenvironment in patients with radiation enteropathy (cases, n = 9) with healthy controls (controls, n = 6). Fecal samples were obtained from all cohorts. In the colonoscopy cohort, intestinal mucosa samples were taken. Metataxonomics (16S rRNA gene) and imputed metataxonomics (Piphillin) were used to characterize the microbiome. Clinician- and patient-reported outcomes were used for clinical characterization. RESULTS: In the acute cohort, we observed a trend for higher preradiotherapy diversity in patients with no self-reported symptoms (P = 0.09). Dynamically, diversity decreased less over time in patients with rising radiation enteropathy (P = 0.05). A consistent association between low bacterial diversity and late radiation enteropathy was also observed, albeit nonsignificantly. Higher counts of Clostridium IV, Roseburia, and Phascolarctobacterium significantly associated with radiation enteropathy. Homeostatic intestinal mucosa cytokines related to microbiota regulation and intestinal wall maintenance were significantly reduced in radiation enteropathy [IL7 (P = 0.05), IL12/IL23p40 (P = 0.03), IL15 (P = 0.05), and IL16 (P = 0.009)]. IL15 inversely correlated with counts of Roseburia and Propionibacterium. CONCLUSIONS: The microbiota presents opportunities to predict, prevent, or treat radiation ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | Print-Electronic; 6500; application/pdf |
| اللغة: | English |
| تدمد: | 1078-0432 1557-3265 |
| العلاقة: | Clinical cancer research : an official journal of the American Association for Cancer Research, 2019, 25 (21), pp. 6487 - 6500; https://repository.icr.ac.uk/handle/internal/3448Test |
| DOI: | 10.1158/1078-0432.ccr-19-0960 |
| الإتاحة: | https://doi.org/10.1158/1078-0432.ccr-19-0960Test https://repository.icr.ac.uk/handle/internal/3448Test |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.73F37409 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 10780432 15573265 |
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| DOI: | 10.1158/1078-0432.ccr-19-0960 |