Usage of rRNA-methyltransferase for site-specific fluorescent labeling
| العنوان: | Usage of rRNA-methyltransferase for site-specific fluorescent labeling |
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| المؤلفون: | Dmitry E. Burakovsky, Timofei S. Zatsepin, M. Tomkuviene, Petr V. Sergiev, Olga A. Dontsova, Olga V. Sergeeva, Saulius Klimašauskas |
| المصدر: | Moscow University Chemistry Bulletin. 67:88-93 |
| بيانات النشر: | Allerton Press, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Fluorescent labelling, Methyltransferase, Biochemistry, biology, Chemistry, biology.protein, Functional activity, General Chemistry, Ribosomal RNA, Fluorescence, Ribosome, Cofactor, Cycloaddition |
| الوصف: | The possibility of using an S-adenosylmethionine analog, i.e., pent-2-en-4-ynyl S-adenosylhomocysteine (AduEnYn), as an rRNA methyltransferase cofactor has been investigated. The conditions for the cycloaddition reaction of the fluorescent label to the S-adenosylmethionine analog were chosen. The functional activity of E. coli ribosomes was tested under different conditions. It was found that the introduction of the alkynyl radical occurred successfully and did not affect the functional activity of the ribosome; however, the inactivation of the ribosome occurred during the following cycloaddition reaction. |
| تدمد: | 1935-0260 0027-1314 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::826907b6e9df841d4e4f69d06fc97aafTest https://doi.org/10.3103/s0027131412020058Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........826907b6e9df841d4e4f69d06fc97aaf |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19350260 00271314 |
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