Inhibition of HBV-induced angiogenesis by ibuprofen: Role of HBx
| العنوان: | Inhibition of HBV-induced angiogenesis by ibuprofen: Role of HBx |
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| المؤلفون: | William Wei Ning Chen, Jianhua Zhang |
| المساهمون: | School of Chemical and Biomedical Engineering |
| المصدر: | Interventional Medicine and Applied Science. 4:21-31 |
| بيانات النشر: | Akademiai Kiado Zrt., 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | biology, business.industry, Angiogenesis, General Medicine, Angiogenic Proteins, medicine.disease, Hbv replication, Ibuprofen, digestive system diseases, Virus, HBx, Hepatocellular carcinoma, Immunology, Cancer research, biology.protein, Medicine, Science::Medicine [DRNTU], business, Interleukin 6, medicine.drug |
| الوصف: | Chronic hepatitis B virus (HBV) carriers may develop hepatocellular carcinoma (HCC) by a wide range of mechanisms including angiogenesis. We show that HBV replication induces the expression of angiogenic proteins interleukin 6 (IL6) and cyclooxygenase-2 (Cox2). Interestingly, ibuprofen (a Cox2 inhibitor) is found to attenuate the levels of IL6 and Cox 2 which are induced by HBV replication.The mechanism of attenuation of angiogenic proteins by ibuprofen was further investigated. Our results show that HBx is involved in the increase of the expression of Cox2 through the NFκB pathway. However, the expression of Cox2 is decreased when the HBx-expressing cells are incubated with ibuprofen. The contrasting effect of HBx on Cox2 is found to be determined by differential dimer formation among the members of the NFκB family of proteins, including NFκB, RelA, and C-rel. Specifically, HBx alone results in dimer formation between NFκB and RelA, while the combined presence of HBx and ibuprofen leads to the formation of NFκB and C-rel. Additional information on the interaction network involving HBx, ibuprofen, and NFκB pathways is revealed by two-dimensional liquid chromatography-tandem mass spectrometry proteomics analysis. Taken together, our findings provide new insights on the angiogenesis induced by HBV replication. |
| تدمد: | 2061-5094 2061-1617 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af5b05951c55a3b2febc72d055c03d03Test https://doi.org/10.1556/imas.4.2012.1.5Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....af5b05951c55a3b2febc72d055c03d03 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20615094 20611617 |
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