Overexpression of hemA and hemL in Bacillus subtilis Promotes Overexpression of 5-aminolevulinic Acid
| العنوان: | Overexpression of hemA and hemL in Bacillus subtilis Promotes Overexpression of 5-aminolevulinic Acid |
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| المؤلفون: | Zhaowei Ye, Haigang Wu, Yuesheng Gong, Jicheng Liu, Jinni Liu |
| المصدر: | Indian Journal of Animal Research. |
| بيانات النشر: | Agricultural Research Communication Center, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Acid content, Expression vector, General Veterinary, biology, ATP synthase, Chemistry, Maltose, Bacillus subtilis, Reductase, biology.organism_classification, chemistry.chemical_compound, Biosynthesis, Biochemistry, biology.protein, Animal Science and Zoology |
| الوصف: | Bacillus subtilis strains were constructed that overexpressed the glutamyl t-RNA reductase (HemA) and glutamyl t-RNA synthase (HemL). The target proteins accounted for 11 and 13% of the total soluble protein in maltose induced extracts, respectively. The culture media from these strains was red with wavelength maxima near 400 nm indicating elevated levels of porphyrins. The 5-aminolevulinic acid content for these strains reached 42.57 and 39.78 mg/L after 24 h of induced growth and 65.23 and 68.45 mg/L, respectively, after 60 h. Therefore, hemA and hemL overexpression enhanced the biosynthesis of 5-aminolevulinic acid and these strains can be used for its purification. |
| تدمد: | 0976-0555 0367-6722 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::3d14a5aa8e1691248c744c5c650dbf7aTest https://doi.org/10.18805/ijar.b-1193Test |
| رقم الانضمام: | edsair.doi...........3d14a5aa8e1691248c744c5c650dbf7a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09760555 03676722 |
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