دورية أكاديمية
Inhibitory effect of chitosan oligosaccharide on human hepatoma cells in vitro
| العنوان: | Inhibitory effect of chitosan oligosaccharide on human hepatoma cells in vitro |
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| المؤلفون: | Liu, Likun, Xin, Yi, Liu, Jia, Zhang, Ershao, Li, Weiling |
| المصدر: | African Journal of Traditional, Complementary and Alternative Medicines; Vol 14, No 4 (2017); 272-277 ; 0189-6016 |
| بيانات النشر: | African Ethnomedicines Network (Nigeria) |
| سنة النشر: | 2017 |
| المجموعة: | AJOL - African Journals Online |
| مصطلحات موضوعية: | Chitosan oligochitosan, Hepatoma cells, Apoptosis, Bcl-2, Caspase-3 |
| الوصف: | Background: Chitosan oligosaccharide, the degradation products of chitin, was reported to have a wide range of physiological functions and biological activities. In this study, we explored the inhibitory effect of Chitosan oligosaccharide on human hepatoma cellsMaterials and Methods: MTT assay was applied to detect cell viability of the human hepatoma cells treated with Chitosan oligosaccharide. Flow cytometric analysis was used to investigate the apoptosis of the human hepatoma cells treated with Chitosan oligosaccharide. We employed western blot to investigate the underlying mechanisms involved in the apoptosis.Results: Our data indicated that chitosan oligosaccharide dose-dependently inhibited the growth of hepatoma cells and induced apoptosis. On the molecular level, chitosan oligosaccharide decreased Bcl-2 and increased Caspase-3 expression which may be related to the apoptosis of hepatoma cells.Conclusion: Our results provide an experimental basis for the clinical development of Chitosan oligosaccharide as a novel anti-hepatoma drug.Keywords: Chitosan oligochitosan, Hepatoma cells, Apoptosis, Bcl-2, Caspase-3 |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://www.ajol.info/index.php/ajtcam/article/view/159768/149338Test; https://www.ajol.info/index.php/ajtcam/article/view/159768Test |
| DOI: | 10.21010/ajtcam.v14i4.30 |
| الإتاحة: | https://doi.org/10.21010/ajtcam.v14i4.30Test https://www.ajol.info/index.php/ajtcam/article/view/159768Test |
| حقوق: | Submission of a manuscript implies: that the work described has not been published before (except in the form of an abstract or as part of a published lecture, or thesis) that it is not under consideration for publication elsewhere; that if and when the manuscript is accepted for publication, the authors agree to automatic transfer of the copyright to the publisher. |
| رقم الانضمام: | edsbas.B42ACE43 |
| قاعدة البيانات: | BASE |
| DOI: | 10.21010/ajtcam.v14i4.30 |
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