التفاصيل البيبلوغرافية
| العنوان: |
铜死亡相关LncRNA预测胃癌的预后及免疫反应. |
| العنوان البديل: |
Cuproptosis-related LncRNA Predict Prognosis and Immune Response in Gastric Cancer. |
| المؤلفون: |
白红艳1, 王 硕1, 费素娟1, 苗 蓓1 miaobei@xzhmu.edu.cn |
| المصدر: |
Journal of Laser Biology / Acta Laser Biology Sinica. Oct2023, Vol. 32 Issue 5, p460-472. 13p. |
| مصطلحات موضوعية: |
*SOMATIC mutation, *PROGNOSTIC models, *DISEASE risk factors, *STOMACH cancer, *CANCER prognosis, *PROGRAMMED cell death 1 receptors, *SURVIVAL analysis (Biometry), *IMMUNOCOMPUTERS |
| الملخص (بالإنجليزية): |
Based on bioinformatics analysis, a prognosis-related prediction model was constructed to explore the relationship between copper death-related LncRNA and gastric cancer (GC) in immunity and prognosis. RNA sequencing and clinical data of gastric cancer patients were downloaded from the Cancer Genome Atlas (TCGA) database, copper death-related LncRNA were screened based on co-expression analysis, and a copper death-related LncRNA risk prediction model closely related to the prognosis of gastric cancer patients was constructed by LASSO regression and multivariate Cox regression analysis, and the risk scores of all gastric cancer patient samples were calculated. The prognostic prediction performance of the model was confirmed by Kaplan-Meier survival analysis, regression analysis and ROC curve, and the correlation between risk score and pathway enrichment analysis, immune infiltrating cells, immune checkpoint genes, somatic gene mutations and anticancer drug sensitivity was analyzed. The results of differential expression analysis showed that the expression of LncRNA HAGLR in tumor tissues was upregulated. The expression of LncRNA HAGLR in cancer tissues and paracancerous tissues in 69 patients with radical surgery was detected by quantitative real-time PCR (qRT-PCR). The qRT-PCR results showed that the expression of HAGLR in gastric cancer tissues was upregulated compared with adjacent tissues, and was significantly correlated with tumor size, the depth of invasion, clinical stage, degree of differentiation and lymph node metastasis (P<0.05). The prognostic model constructed from copper death-related LncRNA has high prognostic value and is significantly related to the heterogeneity of immune cell infiltration, and has certain clinical value in predicting the effect of immunotherapy and guiding the selection of chemotherapy drugs in patients. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
基于生物信息学分析构建预后相关的预测模型, 探讨铜死亡相关LncRNA与胃癌（GC）在免疫和预后方 面的关系。从癌症基因组图谱（TCGA）数据库下载胃癌患者的RNA测序和临床数据, 基于共表达分析筛选出铜 死亡相关LncRNA, 通过LASSO 回归和多因素Cox 回归分析构建出与胃癌预后密切相关的铜死亡相关LncRNA 风险预测模型, 并计算所有胃癌患者样本的风险评分。通过Kaplan-Meier 生存分析、回归分析和受试者工作特征 （ROC）曲线等证实模型的预后预测性能, 并分析风险评分与通路富集分析、免疫浸润细胞、免疫检查点基因、体 细胞基因突变及抗癌药物敏感性的相关性。差异表达分析结果表明, LncRNA HAGLR在肿瘤组织中的表达上调。 通过实时荧光定量PCR（qRT-PCR）检测LncRNA HAGLR在69例行根治性手术胃癌患者的癌组织及癌旁组织的表 达。结果表明, 相比于癌旁组织, 胃癌组织中HAGLR表达上调, 且与肿瘤大小、浸润深度、肿瘤TNM分期、分化程 度及淋巴结转移成明显相关性（P<0.05）。本研究构建的铜死亡相关LncRNA预后模型具有较高的预测价值, 并 且与免疫细胞浸润异质性明显相关, 在预测患者免疫治疗效果及指导化疗药物选择方面具有一定的临床价值. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |
