التفاصيل البيبلوغرافية
| العنوان: |
Non-classical circulating monocytes expressing high levels of microsomal prostaglandin E2 synthase-1 tag an aberrant IFN-response in systemic sclerosis. |
| المؤلفون: |
Villanueva-Martin, Gonzalo1 (AUTHOR), Acosta-Herrera, Marialbert1,2 (AUTHOR), Carmona, Elio G.1,2 (AUTHOR), Kerick, Martin1 (AUTHOR), Ortego-Centeno, Norberto2,3 (AUTHOR), Callejas-Rubio, Jose Luis2 (AUTHOR), Mages, Norbert4 (AUTHOR), Klages, Sven4 (AUTHOR), Börno, Stefan4 (AUTHOR), Timmermann, Bernd4 (AUTHOR), Bossini-Castillo, Lara1,5,6 (AUTHOR) lbossinicastillo@ugr.es, Martin, Javier1 (AUTHOR) javiermartin@ipb.csic.es |
| المصدر: |
Journal of Autoimmunity. Nov2023, Vol. 140, pN.PAG-N.PAG. 1p. |
| مصطلحات موضوعية: |
*SYSTEMIC scleroderma, *DINOPROSTONE, *MONOCYTES, *DENDRITIC cells, *CONNECTIVE tissues, *ANTINUCLEAR factors |
| مستخلص: |
Systemic sclerosis (SSc) is a complex disease that affects the connective tissue, causing fibrosis. SSc patients show altered immune cell composition and activation in the peripheral blood (PB). PB monocytes (Mos) are recruited into tissues where they differentiate into macrophages, which are directly involved in fibrosis. To understand the role of CD14+ PB Mos in SSc, a single-cell transcriptome analysis (scRNA-seq) was conducted on 8 SSc patients and 8 controls. Using unsupervised clustering methods, CD14+ cells were assigned to 11 clusters, which added granularity to the known monocyte subsets: classical (cMos), intermediate (iMos) and non-classical Mos (ncMos) or type 2 dendritic cells. NcMos were significantly overrepresented in SSc patients and showed an active IFN-signature and increased expression levels of PTGES , in addition to monocyte motility and adhesion markers. We identified a SSc-related cluster of IRF7 + STAT1 + iMos with an aberrant IFN-response. Finally, a depletion of M2 polarised cMos in SSc was observed. Our results highlighted the potential of PB Mos as biomarkers for SSc and provided new possibilities for putative drug targets for modulating the innate immune response in SSc. [Display omitted] • Non-classical monocytes in SSc were characterised by a high IFN-response signature and the upregulation of prostaglandin E2 synthesis. • IRF7+ STAT1+ intermediate monocytes contributed especially to SSc. • M2 polarised classical monocytes were depleted in the blood of SSc patients. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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