التفاصيل البيبلوغرافية
| العنوان: |
Tissue engineering for full-thickness burns: A dermal substitute from bench to bedside |
| المؤلفون: |
Kellouche, Sabrina1, Martin, Christophe2, Korb, Gregory3, Rezzonico, Roger4, Bouard, Delphine5, Benbunan, Marc6, Dubertret, Louis7, Soler, Christophe2, Legrand, Chantal1, Dosquet, Christine1,6 christine.dosquet@sls.aphp.fr |
| المصدر: |
Biochemical & Biophysical Research Communications. Nov2007, Vol. 363 Issue 3, p472-478. 7p. |
| مصطلحات موضوعية: |
*BLOOD platelets, *CONNECTIVE tissues, *EXTRACELLULAR matrix proteins, *FIBROBLASTS |
| مستخلص: |
Abstract: Our aim was to obtain a viable and easily available dermal substitute (DS) for the definitive coverage of full-thickness burns. A DS composed of a collagen–glycosaminoglycan–chitosan dermal matrix (DM) colonized with foreskin fibroblasts (FF) is described. FF-colonized DS were compared to the DM seeded with adult dermal fibroblasts (DF). FF-colonized DS expressed more fibrillin and tropoelastin than that with DF. Reconstructed skin obtained with both FF- and DF-colonized DS similarly expressed laminin-5 and collagen VII at the dermal–epidermal junction. Both FF- and DF-colonized DS produced cutaneous wound healing mediators in a dose-dependent manner in the presence of platelet lysate. After freeze-thawing, the FF-colonized DS were recovered in culture and retained their ability to produce vascular endothelial growth factor. Grafting of DS into nude rats achieved a complete healing of a dermal–epidermal lesion with a good epidermalization. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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