التفاصيل البيبلوغرافية
| العنوان: |
The vanilloid capsaicin induces IL-6 secretion in prostate PC-3 cancer cells |
| المؤلفون: |
Malagarie-Cazenave, Sophie sophie.malagarie@gmail.com, Olea-Herrero, Nuria1, Vara, Diana1, Morell, Cecilia1, Díaz-Laviada, Inés1 |
| المصدر: |
Cytokine. Jun2011, Vol. 54 Issue 3, p330-337. 8p. |
| مصطلحات موضوعية: |
*CAPSAICIN, *INTERLEUKIN-6, *PROSTATE, *PROSTATE cancer, *CANCER cells, *CARCINOGENESIS, *PEPPERS |
| مستخلص: |
Abstract: Capsaicin (8-methyl-N-vanillyl-6-nonenamide), a constituent of green and red peppers, has been linked with suppression of tumorigenesis through a mechanism that is not well understood. In the present study, we examined the effects of capsaicin on the production of the cytokine interleukin (IL)-6 by PC-3 cells at both protein and mRNA levels which were evaluated by ELISA and real-time PCR, respectively. Capsaicin-treated PC-3 cells increased the synthesis and secretion of IL-6 which was abrogated by the transient receptor potential vanilloid receptor subtype 1 (TRPV1) antagonist capsazepine, as well as by inhibitors of PKC-α, phosphoinositol-3 phosphate kinase (PI-3K), Akt and extracellular signal-regulated protein kinase (ERK). We analyzed the role of capsaicin in the tumor necrosis factor (TNF)-α secretion by PC-3 cells which was increased at shorter times than IL-6 production. Furthermore, incubation of PC-3 cells with an anti-TNF-α antibody blocked the capsaicin-induced IL-6 secretion. These results raise the possibility that capsaicin-mediated IL-6 increase in prostate cancer PC-3 cells is regulated at least in part by TNF-α secretion and signaling pathway involving Akt, ERK and PKC-α activation. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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