التفاصيل البيبلوغرافية
| العنوان: |
Induction of the endoplasmic reticulum stress protein GADD153/CHOP by capsaicin in prostate PC-3 cells: A microarray study |
| المؤلفون: |
Sánchez, Ana María1, Martínez-Botas, Javier2, Malagarie-Cazenave, Sophie1, Olea, Nuria1, Vara, Diana1, Lasunción, Miguel Angel1,2, Díaz-Laviada, Inés1 ines.diazlaviada@uah.es |
| المصدر: |
Biochemical & Biophysical Research Communications. Aug2008, Vol. 372 Issue 4, p785-791. 7p. |
| مصطلحات موضوعية: |
*HEAT shock proteins, *PROTEIN microarrays, *CAPSAICIN, *RNA |
| مستخلص: |
Abstract: The effect of capsaicin, main pungent ingredient of hot chilli peppers, in the gene expression profile of human prostate PC-3 cancer cells has been analyzed using a microarray approach. We identified 10 genes that were down-regulated and five genes that were induced upon capsaicin treatment. The data obtained from microarray analysis were then validated using quantitative real-time PCR assays and Western blot analysis. The most remarkable change was the up-regulation of GADD153/CHOP, an endoplasmic reticulum stress-regulated gene. Activation of GADD153/CHOP protein was corroborated by immunofluorescence and Western blot. We then tested the contribution of GADD153/CHOP to protection against capsaicin-induced cell death using RNA interference. Blockage of GADD153/CHOP expression by small interfering RNA, significantly reduced capsaicin-induced cell death in PC-3 cells. Taken together, these results suggested that capsaicin induces the antiproliferative effect through a mechanism facilitated by ER stress in prostate PC-3 cells. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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