Milrinone-induced postconditioning reduces hepatic ischemia-reperfusion injury in rats: the roles of phosphatidylinositol 3-kinase and nitric oxide
| العنوان: | Milrinone-induced postconditioning reduces hepatic ischemia-reperfusion injury in rats: the roles of phosphatidylinositol 3-kinase and nitric oxide |
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| المؤلفون: | Reiko Tosaka, Koji Sumikawa, Takuji Maekawa, Masahiro Nakashima, Shinya Tosaka, Tomomi Toyoda, Sungsam Cho, Susumu Eguchi |
| المصدر: | Journal of Surgical Research. 186(1):446-451 |
| بيانات النشر: | Academic Press Inc., 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Male, Phosphodiesterase 3, Ischemia, Ischemia-reperfusion injury, Pharmacology, Nitric Oxide, Phosphodiesterase 3 Inhibitors, Nitric oxide, Wortmannin, Phosphatidylinositol 3-Kinases, chemistry.chemical_compound, medicine, Animals, Rats, Wistar, Ischemic Postconditioning, biology, business.industry, Nitric oxide synthase, Alanine Transaminase, medicine.disease, Cyclic AMP-Dependent Protein Kinases, Rats, chemistry, Liver, Reperfusion Injury, Anesthesia, biology.protein, Milrinone, Surgery, Liver function, business, Reperfusion injury, Phosphatidylinositol 3-kinase, medicine.drug |
| الوصف: | Background Ischemic postconditioning (PostC) protects the liver against ischemia-reperfusion (IR) injury. Milrinone, a phosphodiesterase 3 inhibitor, has been reported to exhibit preconditioning properties against hepatic IR injury; however, its PostC properties remain unknown. This study investigated whether milrinone has PostC properties against hepatic IR injury and the roles of phosphatidylinositol 3-kinase (PI3K) and nitric oxide synthase (NOS). Materials and methods Male Wistar rats were separated into six groups: (1) group S: animals that underwent sham operation without ischemia, (2) group C: ischemia followed by reperfusion with no other intervention, (3) group M: milrinone administered immediately after reperfusion, (4) group MW: wortmannin, a PI3K inhibitor, injected before milrinone administration, (5) group MN: l-NAME, a NOS inhibitor, injected before milrinone administration, and (6) group MD, milrinone administered 30 min after reperfusion. Except for group S, all groups underwent 1 h of warm ischemia of median and left lateral lobes, followed by 5 h of reperfusion. Biochemical liver function analysis and histologic examination were performed. Results Serum aspartate aminotransferase, alanine aminotransferase, and lactic dehydrogenase levels, histologic damage scores, and apoptotic rate in group M were significantly lower than those in group C. The inhibition of PI3K or NOS prevented this protective effect. Milrinone administered 30 min after reperfusion did not show obvious protective effects. Conclusions Milrinone-induced PostC protects against hepatic IR injury when it is administered immediately after reperfusion, and PI3K and NOS may play an important role in this protective effect. Journal of Surgical Research, 186(1), pp.446-451; 2014 |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0022-4804 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89f78482acc7307e84b0bcad868a7f8bTest http://hdl.handle.net/10069/34073Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....89f78482acc7307e84b0bcad868a7f8b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00224804 |
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