التفاصيل البيبلوغرافية
| العنوان: |
The fine art of integral membrane protein crystallisation. |
| المؤلفون: |
Birch, James1, Axford, Danny1, Foadi, James1, Meyer, Arne1, Eckhardt, Annette1, Thielmann, Yvonne1, Moraes, Isabel1 isabel.moraes@npl.co.uk |
| المصدر: |
Methods. Sep2018, Vol. 147, p150-162. 13p. |
| مصطلحات موضوعية: |
*MEMBRANE proteins, *SPECTRUM analysis, *ELECTROMAGNETIC waves, *CRYSTALLOGRAPHY, *CONDENSED matter physics |
| مستخلص: |
Highlights • Overview of the most recent advances in membrane protein crystallisation. • Analysis of membrane protein-detergent complexes by in situ dynamic light scattering. • Characterisation of membrane protein-detergent complexes by size-exclusion chromatography multi-angle light scattering. • Membrane protein serial crystallography. • Data collection and management from multiple membrane protein crystals. Abstract Integral membrane proteins are among the most fascinating and important biomolecules as they play a vital role in many biological functions. Knowledge of their atomic structures is fundamental to the understanding of their biochemical function and key in many drug discovery programs. However, over the years, structure determination of integral membrane proteins has proven to be far from trivial, hence they are underrepresented in the protein data bank. Low expression levels, insolubility and instability are just a few of the many hurdles one faces when studying these proteins. X-ray crystallography has been the most used method to determine atomic structures of membrane proteins. However, the production of high quality membrane protein crystals is always very challenging, often seen more as art than a rational experiment. Here we review valuable approaches, methods and techniques to successful membrane protein crystallisation. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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