التفاصيل البيبلوغرافية
| العنوان: |
Sp1 promotes ovarian cancer cell migration through repressing miR-335 expression. |
| المؤلفون: |
Wang, Shaohai1 (AUTHOR), Li, Yuan2 (AUTHOR), Sun, Si1 (AUTHOR), Cai, Jing1 (AUTHOR), Cao, Jin1 (AUTHOR) Jin_Cao@hust.edu.cn |
| المصدر: |
Biochemical & Biophysical Research Communications. Mar2020, Vol. 524 Issue 1, p211-216. 6p. |
| مصطلحات موضوعية: |
*CANCER cell migration, *OVARIAN cancer, *CELL migration inhibition, *CELL migration, *TRANSCRIPTION factors, *CANCER prognosis |
| مستخلص: |
Decreased miR-335 has been reported in a variety of cancers. We previously showed that miR-335 played an important role in ovarian cancer metastasis and prognosis. However, miR-335 is down-regulated in ovarian cancer by mechanisms that remain unclear. In silico analysis identified putative transcription factor specificity protein 1 (SP1) transcription factor binding sites in the miR-335 promoter. To investigate the relation between SP1 and miR-335, qRT-PCR was performed. Our results showed both Sp1 knockdown and mithramycin A increased miR-335 expression in ovarian cancer cell lines. Luciferase reporter assays indicated that Sp1 knockdown increased miR-335 transcriptional activity. ChIP experiments showed that Sp1 bound directly to miR-335 promoter. Moreover, transwell migration and wound-healing assays showed that Sp1 knockdown resulted in inhibited cell migration, which was in turn mitigated by miR-335 inhibitor. We propose that miR-335 was negatively regulated by SP1, which in turn contributes to miR-335 deregulation and tumor cells migration. • Mir-335 is negatively regulated by Sp1. • Sp1 promotes ovarian cancer migration via down-regulating miR-335. • Knockdown of Sp1 reduces mRNA expression of DNMTs in EOC cells. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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