التفاصيل البيبلوغرافية
| العنوان: |
How many molecules of mitochondrial complex I are in a cell? |
| المؤلفون: |
Ansari, Fariha1 (AUTHOR), Yoval, Belem1 (AUTHOR), Niatsetskaya, Zoya2,3 (AUTHOR), Ten, Vadim2,3 (AUTHOR), Wittig, Ilka4,5 (AUTHOR), Galkin, Alexander1 (AUTHOR) alg2057@med.cornell.edu |
| المصدر: |
Analytical Biochemistry. Jun2022, Vol. 646, pN.PAG-N.PAG. 1p. |
| مصطلحات موضوعية: |
*MITOCHONDRIA, *MOLECULES, *REPERFUSION injury, *MITOCHONDRIAL DNA, *FLAVIN mononucleotide, *NAD (Coenzyme), *POLYACRYLAMIDE |
| مستخلص: |
Mitochondrial complex I is the only enzyme responsible for oxidation of matrix NADH and regeneration of NAD+ for catabolism. Nuclear and mtDNA mutations, assembly impairments, and enzyme damage are implicated in inherited diseases, ischemia-reperfusion injury, neurodegeneration, and tumorogenesis. Here we introduce a novel method to measure the absolute content of complex I. The method is based on flavin fluorescence scanning of a polyacrylamide gel after separation of complexes by Clear Native electrophoresis. Using mouse primary astrocytes as an example, we calculated an average value of 2.2 × 105 complex I molecules/cell. Our method can be used for accurate quantification of complex I content. [Display omitted] • We developed a method for the determination of mitochondrial complex I content in cells. • The method has been verified to produce absolute content of the enzyme in a sample. • 2.2 × 105 complex I molecules are present in a single mouse primary astrocyte. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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