دورية أكاديمية
Non-classical circulating monocytes expressing high levels of microsomal prostaglandin E2 synthase-1 tag an aberrant IFN-response in systemic sclerosis
| العنوان: | Non-classical circulating monocytes expressing high levels of microsomal prostaglandin E2 synthase-1 tag an aberrant IFN-response in systemic sclerosis |
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| المؤلفون: | Villanueva-Martín, Gonzalo, Acosta-Herrera, Marialbert, Carmona, Elio G., Kerick, Martin, Ortego-Centeno, Norberto, Callejas-Rubio, José Luis, Mages, Norbert, Börno, Stefan, Timmermann, Bernd, Bossini-Castillo, L., Martin, Javier, Klages, Sven |
| المساهمون: | Junta de Andalucía, Ministerio de Ciencia e Innovación (España), FONDOS FEDER, Instituto de Salud Carlos III |
| بيانات النشر: | Academic Press |
| سنة النشر: | 2023 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Systemic sclerosis, Single-cell transcriptomes, cRNA-seq, Monocyte, CD14 |
| الوصف: | Systemic sclerosis (SSc) is a complex disease that affects the connective tissue, causing fibrosis. SSc patients show altered immune cell composition and activation in the peripheral blood (PB). PB monocytes (Mos) are recruited into tissues where they differentiate into macrophages, which are directly involved in fibrosis. To understand the role of CD14+ PB Mos in SSc, a single-cell transcriptome analysis (scRNA-seq) was conducted on 8 SSc patients and 8 controls. Using unsupervised clustering methods, CD14+ cells were assigned to 11 clusters, which added granularity to the known monocyte subsets: classical (cMos), intermediate (iMos) and non-classical Mos (ncMos) or type 2 dendritic cells. NcMos were significantly overrepresented in SSc patients and showed an active IFN-signature and increased expression levels of PTGES, in addition to monocyte motility and adhesion markers. We identified a SSc-related cluster of IRF7+ STAT1+ iMos with an aberrant IFN-response. Finally, a depletion of M2 polarised cMos in SSc was observed. Our results highlighted the potential of PB Mos as biomarkers for SSc and provided new possibilities for putative drug targets for modulating the innate immune response in SSc. ; This work was supported by the grant P18-RT-4442 funded by Consejería de Transformación Económica, Industria, Conocimiento y Universidades, Junta de Andalucía. “Red de Investigación Cooperativa Orientada a Resultados en Salud'' (RICOR, RD21/0002/003). 115565. LBC was supported by the Spanish Ministry of Science and Innovation through the Juan de la Cierva Incorporación' program (Grant ref. IJC2018-038026-I, funded by MCIN/AEI/10.13039/501,100,011,033), which includes FEDER funds. MAH is a recipient of a Miguel Servet fellowship (CP21/00132) from the Instituto de Salud Carlos III (Spanish Ministry of Science and Innovation). GV-M was funded by the Grant PRE2019-087586 funded by MCIN/AEI/10.13039/501,100,011,033 and by “ESF Investing in your future”. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 0896-8411 |
| العلاقة: | Publisher's version; http://dx.doi.org/10.1016/j.jaut.2023.103097Test; Sí; Journal of Autoimmunity 140: 103097 (2023); http://hdl.handle.net/10261/354839Test |
| DOI: | 10.1016/j.jaut.2023.103097 |
| الإتاحة: | https://doi.org/10.1016/j.jaut.2023.103097Test http://hdl.handle.net/10261/354839Test |
| حقوق: | open |
| رقم الانضمام: | edsbas.4E8C6C88 |
| قاعدة البيانات: | BASE |
| تدمد: | 08968411 |
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| DOI: | 10.1016/j.jaut.2023.103097 |