دورية أكاديمية
Lnc-SMaRT translational regulation of Spire1, a new player in muscle differentiation
| العنوان: | Lnc-SMaRT translational regulation of Spire1, a new player in muscle differentiation |
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| المؤلفون: | Scalzitti S., Mariani D., Setti A., Colantoni A., Lisi M., Bozzoni I., Martone J. |
| المساهمون: | Scalzitti, S., Mariani, D., Setti, A., Colantoni, A., Lisi, M., Bozzoni, I., Martone, J. |
| بيانات النشر: | Academic Press London |
| سنة النشر: | 2022 |
| المجموعة: | Sapienza Università di Roma: CINECA IRIS |
| مصطلحات موضوعية: | DHX36 helicase, G-quadruplex, long ncRNA, myogenesi, post-transcriptional regulation, Acyl-CoA Dehydrogenase, animal, cell differentiation, DEAD-box RNA Helicase, G-Quadruplexe, gene expression regulation, developmental, human, mice, microfilament protein, muscle development, muscle, nerve tissue protein, protein conformation, RNA processing, post-transcriptional, RNA, long noncoding, messenger, RNA-binding protein, transcription factors |
| الوصف: | The destiny of a messenger RNA is determined from a combination of in cis elements, like peculiar secondary structures, and in trans modulators, such as RNA binding proteins and non-coding, regulatory RNAs. RNA guanine quadruplexes belong to the first group: these strong secondary structures have been characterized in many mRNAs, and their stabilization or unwinding provides an additional step for the fine tuning of mRNA stability and translation. On the other hand, many cytoplasmic long non-coding RNAs intervene in post-transcriptional regulation, frequently by direct base-pairing with their mRNA targets. We have previously identified the lncRNA SMaRT as a key modulator of the correct timing of murine skeletal muscle differentiation; when expressed, lnc-SMaRT interacts with a G-quadruplex-containing region of Mlx-γ mRNA, therefore inhibiting its translation by counteracting the DHX36 helicase activity. The “smart” mode of action of lnc-SMaRT led us to speculate whether this molecular mechanism could be extended to other targets and conserved in other species. Here, we show that the molecular complex composed by lnc-SMaRT and DHX36 also includes other mRNAs. We prove that lnc-SMaRT is able to repress Spire1 translation through base-pairing with its G-quadruplex-forming sequence, and that Spire1 modulation participates to the regulation of proper skeletal muscle differentiation. Moreover, we demonstrate that the interaction between DHX36 and lnc-SMaRT is indirect and mediated by the mRNAs present in the complex. Finally, we suggest an extendibility of the molecular mechanism of lnc-SMaRT from the mouse model to humans, identifying potential functional analogues. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/34863993; info:eu-repo/semantics/altIdentifier/wos/WOS:000807264900012; volume:434; issue:2; numberofpages:16; journal:JOURNAL OF MOLECULAR BIOLOGY; http://hdl.handle.net/11573/1610567Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85121271313 |
| DOI: | 10.1016/j.jmb.2021.167384 |
| الإتاحة: | https://doi.org/10.1016/j.jmb.2021.167384Test http://hdl.handle.net/11573/1610567Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.E740F943 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.jmb.2021.167384 |
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