دورية أكاديمية
Interleukin-1beta-induced apoptosis through adenylyl cyclase and ERK1/2 inhibition in primary cultured thyroid cells.
| العنوان: | Interleukin-1beta-induced apoptosis through adenylyl cyclase and ERK1/2 inhibition in primary cultured thyroid cells. |
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| المؤلفون: | El Btaouri, Hassan, Rath, Géraldine, Morjani, Hamid, Schneider, Christophe, Petitfrere, Emmanuelle, Antonicelli, Franck, Martiny, Laurent |
| المصدر: | Biochemical and Biophysical Research Communications, Vol. 339, no.2, p. 469-76 (2006) |
| بيانات النشر: | Academic Press |
| سنة النشر: | 2006 |
| المجموعة: | DIAL@USL-B (Université Saint-Louis, Bruxelles) |
| مصطلحات موضوعية: | Adenylate Cyclase, Interleukin-1, MAP Kinase Signaling System, Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinase 3, Phosphorylation, Swine, Thyroid Gland, Animals, Apoptosis, Caspase 3, Caspases, Cells, Cultured, Ceramides, Cyclic AMP, Humans |
| الوصف: | The programmed cell death plays a crucial role in the regulation of numerous physiological and pathological phenomena. In this study, we show that interleukin-1 beta (IL-1beta) induces an early production of endogenous ceramides via N-sphingomyelinase (N-Smase) as well as an inhibition of adenylyl cyclase activity in pig thyroid cells. This effect is followed by a down-regulation of the extracellular signal-regulated protein kinase (ERK1/2) phosphorylation, an activation of caspase-3, and ends by setting up the programmed cell death. The permeable exogenous C(2)-ceramide reproduces IL-1beta effects on: (i) inhibition of adenylyl cyclase activity, (ii) down-regulation of ERK1/2 phosphorylation, (iii) activation of caspase-3, and (iv) apoptosis in pig thyroid cells. Cell treatment with a PKA inhibitor down-regulates ERK1/2 phosphorylation. Furthermore, inhibition of ERK1/2 signaling pathway by U-0126 enhances caspase-3 activity and sets up programmed cell death. Both IL-1beta and exogenous C(2)-ceramide effects are reproduced by U-0126 so illustrating the implication of ERK1/2 down-regulation in both caspase-3 activation and apoptosis induction. Our study shows for the first time that endogenous ceramides are important second messengers in IL-1beta-induced apoptosis in pig thyroid cells through inhibition of adenylyl cyclase and ERK1/2 activities. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0006-291X 1090-2104 |
| العلاقة: | boreal:127232; http://hdl.handle.net/2078/127232Test; info:pmid/16310170; urn:ISSN:0006-291X; urn:EISSN:1090-2104 |
| DOI: | 10.1016/j.bbrc.2005.10.213 |
| الإتاحة: | https://doi.org/10.1016/j.bbrc.2005.10.213Test http://hdl.handle.net/2078/127232Test |
| حقوق: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.E81895E8 |
| قاعدة البيانات: | BASE |
| تدمد: | 0006291X 10902104 |
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| DOI: | 10.1016/j.bbrc.2005.10.213 |