دورية أكاديمية
Calcium enhances mouse keratinocyte differentiation in vitro to differentially regulate expression of papillomavirus authentic and codon modified L1 genes
| العنوان: | Calcium enhances mouse keratinocyte differentiation in vitro to differentially regulate expression of papillomavirus authentic and codon modified L1 genes |
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| المؤلفون: | Fang, N. X., Gu, W., Ding, J., Saunders, N. A., Frazer, I. H., Zhao, K. N. |
| المساهمون: | Lamb, R. |
| بيانات النشر: | Academic Press |
| سنة النشر: | 2007 |
| المجموعة: | The University of Queensland: UQ eSpace |
| مصطلحات موضوعية: | Calcium, Keratinocytes, Differentiation, Papillomavirus, L1 genes, Expression, 270201 Gene Expression, 321002 Dermatology, C1, 780105 Biological sciences |
| الوصف: | Here, we first wished to establish for mouse primary keratinocytes (KCs) the Ca2+ concentrations that were associated with KC differentiation in vitro. Using the range of Ca2+ concentrations (0–6 mM) to differentiate primary KCs in culture to varying extents for 2 days, we then examined how KC differentiation impacted on expression of papillomavirus (PV) native (Nat) and codon modified (Mod) L1 genes. L1 mRNAs transcribed from either Nat or Mod L1 genes were present in similar amounts in KCs exposed to six Ca2+ concentrations. However, expression of the L1 proteins from two Mod L1 genes were down-regulated, with no L1 signal detected in KCs exposed to 6 mM Ca2+. In contrast, L1 proteins expressed from the two Nat L1 genes were not detectable in KCs without Ca2+, but dramatically up-regulated as the KC cultures exposed to Ca2+ from 0.5 to 2 mM, then down-regulated in KCs exposed to Ca2+ from 4 to 6 mM. The different regulatory roles of the Ca2+ in L1 protein expression from Nat and Mod L1 genes in cultured KCs were confirmed by TGF-β1 experiments. We observed that aminoacyl-tRNAs (aa-tRNAs) from the 2 mM Ca2+-treated KCs only significantly enhanced the Nat L1 mRNAs translation in vitro, suggesting that aa-tRNAs play a differentially regulatory role in translations of the PV Nat and Mod L1 mRNAs. Importantly, the Ca2+ experimental model provides evidence that mouse primary KCs could be transiently infected by BPV1 virus to express L1 mRNA and protein, which is very useful for future HPV virus infection study. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0042-6822 1096-0341 1089-862X |
| العلاقة: | orcid:0000-0003-3360-1390; orcid:0000-0002-2478-3420; orcid:0000-0002-8002-4680; orcid:0000-0001-8076-063X |
| الإتاحة: | https://doi.org/10.1016/j.virol.2007.03.038Test https://espace.library.uq.edu.au/view/UQ:135810Test |
| رقم الانضمام: | edsbas.E3C916BE |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00426822 10960341 1089862X |
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