المؤلفون: LANDRIEL, SOLEDAD CAMINATA, CASTILLO, JULIETA D.L.M., TABOGA, OSCAR A., FERRAROTTI, SUSANA A., GOTTLIEB, ALEXANDRA M., COSTA, HERNÁN

المصدر: Anais da Academia Brasileira de Ciências. January 2019 91(3)

مصطلحات موضوعية: Cyclodextrin glycosyltransferases, housekeeping genes, Paenibacillus, 16S rRNA

الوصف: Cyclodextrin glycosyltransferases (CGTases) are important enzymes in the biotechnology field because they catalyze starch conversion into cyclodextrins and linear oligosaccharides, which are used in food, pharmaceutical and cosmetic industries. The CGTases are classified according to their product specificity in α-, β-, α/β- and γ-CGTases. As molecular markers are the preferred tool for bacterial identification, we employed six molecular markers (16S rRNA, dnaK, gyrB, recA, rpoB and tufA) to test the identification of a CGTase-producing bacterial strain (DF 9R) in a phylogenetic context. In addition, we assessed the phylogenetic relationship of CGTases along bacterial evolution. The results obtained here allowed us to identify the strain DF 9R as Paenibacillus barengoltzii, and to unveil a complex origin for CGTase types during archaeal and bacterial evolution. We postulate that the α-CGTase activity represents the ancestral type, and that the γ-activity may have derived from β-CGTases.

وصف الملف: text/html

الوصول الحر: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500620Test

المؤلفون: Caminata Landriel, Soledad, Castillo, Julieta D. L. M., Taboga, Oscar Alberto, Ferrarotti, Susana Alicia, Gottlieb, Alexandra Marina, Costa, Hernán

المصدر: Anais da Academia Brasileira de Ciências 91 (3) : e20180568. (Octubre 2019)

مصطلحات موضوعية: Paenibacillus, Glicosidasas, Marcadores Genéticos, Actividad Enzimática, Filogenia, Glycosidases, Genetic Markers, Enzyme Activity, Phylogeny

الوصف: Cyclodextrin glycosyltransferases (CGTases) are important enzymes in the biotechnology field because they catalyze starch conversion into cyclodextrins and linear oligosaccharides, which are used in food, pharmaceutical and cosmetic industries. The CGTases are classified according to their product specificity in α-, β-, α/β- and γ-CGTases. As molecular markers are the preferred tool for bacterial identification, we employed six molecular markers (16S rRNA, dnaK, gyrB, recA, rpoB and tufA) to test the identification of a CGTase-producing bacterial strain (DF 9R) in a phylogenetic context. In addition, we assessed the phylogenetic relationship of CGTases along bacterial evolution. The results obtained here allowed us to identify the strain DF 9R as Paenibacillus barengoltzii, and to unveil a complex origin for CGTase types during archaeal and bacterial evolution. We postulate that the α-CGTase activity represents the ancestral type, and that the γ-activity may have derived from β-CGTases. ; Instituto de Biotecnología ; Fil: Caminata Landriel, Soledad. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Química Biológica; Argentina ; Fil: Castillo, Julieta D. L. M. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Química Biológica; Argentina ; Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Ferrarotti, Susana Alicia. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Química Biológica; Argentina. ; Fil: Gottlieb, Alexandra Marina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Costa, Hernán. Universidad Nacional de Luján. Departamento de Ciencias Básicas.Laboratorio de Química Biológica; Argentina. Consejo Nacional de ...

وصف الملف: application/pdf

العلاقة: http://hdl.handle.net/20.500.12123/6671Test; http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500620&lng=en&nrm=iso&tlng=enTest; http://dx.doi.org/10.1590/0001-3765201920180568Test

الإتاحة: https://doi.org/20.500.12123/6671Test
https://doi.org/10.1590/0001-3765201920180568Test
https://hdl.handle.net/20.500.12123/6671Test
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500620&lng=en&nrm=iso&tlng=enTest

المؤلفون: Caminata Landriel, Soledad, Castillo, Julieta de Las Mercedes, Taboga, Oscar Alberto, Ferrarotti, Susana Alicia, Gottlieb, Alexandra Marina, Costa, Hernán

مصطلحات موضوعية: 16S RRNA, CYCLODEXTRIN GLYCOSYLTRANSFERASES, HOUSEKEEPING GENES, PAENIBACILLUS, https://purl.org/becyt/ford/1.6Test, https://purl.org/becyt/ford/1Test

الوصف: Cyclodextrin glycosyltransferases (CGTases) are important enzymes in the biotechnology field because they catalyze starch conversion into cyclodextrins and linear oligosaccharides, which are used in food, pharmaceutical and cosmetic industries. The CGTases are classified according to their product specificity in α-, β-, α/β-and γ-CGTases. As molecular markers are the preferred tool for bacterial identification, we employed six molecular markers (16S rRNA, dnaK, gyrB, recA, rpoB and tufA) to test the identification of a CGTase-producing bacterial strain (DF 9R) in a phylogenetic context. In addition, we assessed the phylogenetic relationship of CGTases along bacterial evolution. The results obtained here allowed us to identify the strain DF 9R as Paenibacillus barengoltzii, and to unveil a complex origin for CGTase types during archaeal and bacterial evolution. We postulate that the α-CGTase activity represents the ancestral type, and that the γ-activity may have derived from β-CGTases. ; Fil: Caminata Landriel, Soledad. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina ; Fil: Castillo de las Mercedes Julieta. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina ; Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina ; Fil: Ferrarotti, Susana Alicia. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina ; Fil: Gottlieb, Alexandra Marina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución. Laboratorio de Citogenética y Evolución; Argentina ; Fil: Costa, Hernán. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones ...

وصف الملف: application/pdf

العلاقة: info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/tnpyjyTest; http://hdl.handle.net/11336/93882Test; Caminata Landriel, Soledad; Castillo, Julieta de Las Mercedes; Taboga, Oscar Alberto; Ferrarotti, Susana Alicia; Gottlieb, Alexandra Marina; et al.; Molecular identification of a cyclodextrin glycosyltransferase-producinmicroorganism and phylogenetic assessment of enzymatic activities; Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 91; 3; 12-2018; 1-11; e20180568; CONICET Digital; CONICET

الإتاحة: https://doi.org/10.1590/0001-3765201920180568Test
http://hdl.handle.net/11336/93882Test