رسالة جامعية
Molecular genetic mechanism of OsFKF1 in flowering time in rice ; 벼 OsFKF1 유전자의 개화기 조절에 대한 분자유전학적 연구
| العنوان: | Molecular genetic mechanism of OsFKF1 in flowering time in rice ; 벼 OsFKF1 유전자의 개화기 조절에 대한 분자유전학적 연구 |
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| المؤلفون: | 한수현 |
| المساهمون: | 백남천, 농업생명과학대학 식물생산과학부 |
| بيانات النشر: | 서울대학교 대학원 |
| سنة النشر: | 2015 |
| المجموعة: | Seoul National University: S-Space |
| مصطلحات موضوعية: | OsFKF1, knockout mutant, flowering time, Ehd2, Ghd7, Ehd1, blue-light response, rice |
| الوقت: | 633 |
| الوصف: | 학위논문 (박사)-- 서울대학교 대학원 : 식물생산과학부, 2015. 8. 백남천. ; Flowering time (or heading date) is a critical trait that determines seasonal and regional adaptation in plants. Many important regulators in photoperiodic flowering pathway are conserved between Arabidopsis thaliana (At) and rice (Oryza sativa ; Os), however some are diversified in their functions in the regulation of flowering time. In the facultative long-day (LD) plant Arabidopsis thaliana, FLAVIN-BINDING, KELCH REPEAT, F-BOX 1 (FKF1), a blue-light receptor, regulates photoperiodic flowering through the transcriptional and post-translational regulation of CONSTANS (CO) under inductive LD conditions. By contrast, the facultative short day (SD) plant rice (Oryza sativa) flowers early under inductive SD and late under non-inductive LD conditions, and the regulatory function of OsFKF1, an ortholog of Arabidopsis FKF1, remains elusive. In this study, we characterized the T-DNA insertion osfkf1 knockout mutants which showed a late flowering phenotype under SD, LD, and natural LD conditions. Transcriptional analysis revealed that OsFKF1 up-regulates expression of the floral activator Early heading date 2 (Ehd2) and down-regulates expression of the floral repressor Grain number, plant height and heading date 7 (Ghd7) under both SD and LD conditions. Consequently, the expression levels of potent floral inducers Early heading date 1 (Ehd1), Heading date 3a (Hd3a) and RICE FLOWERING LOCUS T 1 (RFT1), downstream of Ehd2 and Ghd7, decrease in osfkf1 mutants. Notably, we found that OsFKF1 can up-regulate Ehd1 expression in a blue light-dependent manner, without modulating the expression of Ehd2 and Ghd7. In contrast to the LD-specific floral activator AtFKF1, OsFKF1 likely acts as an autonomous floral activator because it promotes flowering independent of photoperiod, probably via its distinct roles in controlling expression of rice-specific genes including Ehd2, Ghd7, and Ehd1. In contast, OsFKF1 also interacts with OsGI and OsCDF1 (also termed OsDof12) like Arabidopsis ... |
| نوع الوثيقة: | thesis |
| وصف الملف: | application/pdf; 2486434 bytes |
| اللغة: | English |
| ردمك: | 978-0-00-000067-5 0-00-000067-1 |
| العلاقة: | 000000067163; http://hdl.handle.net/10371/121005Test |
| الإتاحة: | http://hdl.handle.net/10371/121005Test |
| رقم الانضمام: | edsbas.FB9F487F |
| قاعدة البيانات: | BASE |
| ردمك: | 9780000000675 0000000671 |
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