دورية أكاديمية
Synthetic glycan ligand excludes CD22 from antigen receptor-containing lipid rafts
| العنوان: | Synthetic glycan ligand excludes CD22 from antigen receptor-containing lipid rafts |
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| المؤلفون: | Yu, Jie, Sawada, Toshihiko, Adachi, Takahiro, Gao, Xiaoming, Takematsu, Hiromu, Kozutsumi, Yasunori, Ishida, Hideharu, Kiso, Makoto, Tsubata, Takeshi |
| المساهمون: | Tsubata, T (reprint author), Tokyo Med & Dent Univ, Sch Biomed Sci, Immunol Lab, Bunkyo Ku, 1-5-45 Yushima, Tokyo 1138510, Japan., Tokyo Med & Dent Univ, Sch Biomed Sci, Immunol Lab, Bunkyo Ku, Tokyo 1138510, Japan., Tokyo Med & Dent Univ, Med Res Inst, Dept Immunol, Tokyo, Japan., Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Immunol, Beijing 100871, Peoples R China., Kyoto Univ, Grad Sch Biostudies, Lab Membrane Biochem & Biophys, Kyoto, Japan., RIKEN, RIKEN Frontier Res Syst, Supra Biomol Syst Res Grp, Wako, Saitama, Japan., Gifu Univ, Fac Appl Biol Sci, Gifu, Japan., Tokyo Med & Dent Univ, Sch Biomed Sci, Immunol Lab, Bunkyo Ku, 1-5-45 Yushima, Tokyo 1138510, Japan. |
| المصدر: | SCI |
| بيانات النشر: | 生物化学与生物物理学研究通讯 |
| سنة النشر: | 2007 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | B lymphocyte, B cell antigen receptor, CD22, Siglec, alpha 2,6-linked sialic acid, sialoside, lipid rafts, B-CELL RECEPTOR, N-GLYCOLYLNEURAMINIC ACID, SIALIC ACIDS, IMMUNOGLOBULIN SUPERFAMILY, ACETYLNEURAMINIC ACID, ACTIVATION, ADHESION, BINDING, MEMBRANE, SIALOADHESIN |
| الوصف: | CD22/Siglec-2 is a B cell membrane-bound lectin that recognizes glycan ligands containing alpha 2,6-linked sialic acid, and negatively regulates signaling through the B cell antigen receptor (BCR). Previous studies demonstrated that synthetic sialosides that bind to CD22 augment BCR signaling by inhibiting CD22-mediated BCR regulation. Here we demonstrate that, after antigen stimulation, CD22 forms a cap together with BCR, and translocates to lipid rafts. Both co-capping of CD22 with BCR and translocation of CD22 to lipid rafts were markedly blocked by a synthetic alpha 2,6-linked sialic acid, Neu5Gc alpha 2-6Gal beta SE. These results strongly suggest that synthetic glycan ligand excludes CD22 from BCR-containing lipid rafts. Because CD22-mediated signal regulation requires phosphorylation of CD22 by Lyn that localizes in lipid rafts and is activated by BCR, synthetic glycan ligand regulates localization of CD22 crucial for signal regulation. (c) 2007 Elsevier Inc. All rights reserved. ; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000248502700009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Test ; Biochemistry & Molecular Biology ; Biophysics ; SCI(E) ; 13 ; ARTICLE ; 4 ; 759-764 ; 360 |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | English |
| تدمد: | 0006-291X 1090-2104 |
| العلاقة: | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2007,360,(4),759-764.; 965547; http://hdl.handle.net/20.500.11897/249703Test; WOS:000248502700009 |
| DOI: | 10.1016/j.bbrc.2007.06.110 |
| الإتاحة: | https://doi.org/20.500.11897/249703Test https://doi.org/10.1016/j.bbrc.2007.06.110Test https://hdl.handle.net/20.500.11897/249703Test |
| رقم الانضمام: | edsbas.2EE60D83 |
| قاعدة البيانات: | BASE |
| تدمد: | 0006291X 10902104 |
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| DOI: | 10.1016/j.bbrc.2007.06.110 |