دورية أكاديمية
A synthetic cationic antimicrobial peptide inhibits inflammatory response and the NLRP3 inflammasome by neutralizing LPS and ATP
| العنوان: | A synthetic cationic antimicrobial peptide inhibits inflammatory response and the NLRP3 inflammasome by neutralizing LPS and ATP |
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| المؤلفون: | Chih-Yu Hsieh, Lan-Hui Li, Wei-Chih Dong, Wan-Tze Chen, Kuo-Feng Hua, Tz-Chuen Ju, Wei-Jung Chen |
| المصدر: | 10.1371/journal.pone.0182057 ; 28750089 ; oai:pubmedcentral.nih.gov:5531531 ; 2739624572 ; oai:doaj.org/article:49c6caee184945d59606c208ae57c072 ; 10|openaire____::081b82f96300b6a6e3d282bad31cb6e2 ; openaire____::1256f046-bf1f-4afc-8b47-d0b147148b18 ; 10|opendoar____::eda80a3d5b344bc40f3bc04f65b7a357 ; 10|opendoar____::8b6dd7db9af49e67306feb59a8bdc52c ; 10|doajarticles::830e55b42c4aaa815c19cfa4f2e5855e ; 10|openaire____::8ac8380272269217cb09a928c8caa993 ; 10|openaire____::5f532a3fc4f1ea403f37070f59a7a53a ; 10|driver______::bee53aa31dc2cbb538c10c2b65fa5824 ; 10|openaire____::9e3be59865b2c1c335d32dae2fe7b254 ; 10|openaire____::55045bd2a65019fd8e6741a755395c8c ; 10|openaire____::806360c771262b4d6770e7cdf04b5c5a |
| بيانات النشر: | Public Library of Science (PLoS) |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Research Article, Biology and Life Sciences, Cell Biology, Cellular Types, Animal Cells, Blood Cells, White Blood Cells, Macrophages, Immune Cells, Immunology, Medicine and Health Sciences, Immune System Proteins, Inflammasomes, Biochemistry, Proteins, Immune Response, Inflammation, Diagnostic Medicine, Signs and Symptoms, Pathology and Laboratory Medicine, Physiology, Physiological Processes, Secretion, Signal Transduction, Cell Signaling, Membrane Receptor Signaling, Immune Receptor Signaling, Research and Analysis Methods, Immunologic Techniques, Immunoassays |
| الوصف: | Antimicrobial peptides (AMPs) are one of the most important defense mechanisms against bacterial infections in insects, plants, non-mammalian vertebrates, and mammals. In the present study, a class of synthetic AMPs was evaluated for anti-inflammatory activity. One cationic AMP, GW-A2, demonstrated the ability to inhibit the expression levels of nitric oxide (NO), inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in lipopolysaccharide (LPS)-activated macrophages. GW-A2 reduced LPS-induced increases in the phosphorylation of mitogen-activated protein kinase and protein kinase C-α/δ and the activation of NF-κB. GW-A2 also inhibited NLRP3 inflammasome activation induced by LPS and ATP. Furthermore, in the mice injected with LPS, GW-A2 reduced (1) the concentration of IL-1β, IL-6 and TNF-α in the serum; (2) the concentration of TNF-α in the peritoneal lavage; (3) the expression levels of iNOS, COX-2 and NLRP3 in the liver and lung; (4) the infiltration of polymorphonuclear neutrophils in the liver and lung. The underlying mechanisms for the anti-inflammatory activity of GW-A2 were found to be partially due to LPS and ATP neutralization. These results provide insights into how GW-A2 inhibits inflammation and the NLRP3 inflammasome and provide a foundation for the design of rational therapeutics for inflammation-related diseases. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | https://dx.doi.org/10.1371/journal.pone.0182057Test; http://europepmc.org/articles/PMC5531531Test; http://dx.plos.org/10.1371/journal.pone.0182057Test; http://dx.doi.org/10.1371/journal.pone.0182057Test; https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0182057&type=printableTest; https://pubmed.ncbi.nlm.nih.gov/28750089Test/; https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0182057Test; https://www.ncbi.nlm.nih.gov/pubmed/28750089Test; https://paperity.org/p/80308391/a-synthetic-cationic-antimicrobial-peptide-inhibits-inflammatory-response-and-the-nlrp3Test; https://dx.plos.org/10.1371/journal.pone.0182057Test; https://academic.microsoft.com/#/detail/2739624572Test; http://europepmc.org/articles/PMC5531531?pdf=renderTest |
| DOI: | 10.1371/journal.pone.0182057 |
| DOI: | 10.1371/journal.pone.0182057&type=printable |
| الإتاحة: | https://doi.org/10.1371/journal.pone.0182057Test http://europepmc.org/articles/PMC5531531Test https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0182057&type=printableTest https://pubmed.ncbi.nlm.nih.gov/28750089Test/ https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0182057Test https://www.ncbi.nlm.nih.gov/pubmed/28750089Test https://paperity.org/p/80308391/a-synthetic-cationic-antimicrobial-peptide-inhibits-inflammatory-response-and-the-nlrp3Test https://academic.microsoft.com/#/detail/2739624572Test http://europepmc.org/articles/PMC5531531?pdf=renderTest |
| حقوق: | lic_creative-commons |
| رقم الانضمام: | edsbas.398367AB |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pone.0182057 |
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