نتائج البحث - "Nicholas Feasey"

الوصف: The fitness cost to bacteria of acquisition of resistance determinants is critically under-investigated, and the identification and exploitation of these fitness costs may lead to novel therapeutic strategies that prevent the emergence of antimicrobial resistance. Here we used Escherichia coli and amoxicillin–clavulanic acid (AMC) resistance as a model to understand how the artificial environments utilized in studies of bacterial fitness could affect the emergence of resistance and associated fitness costs. Further, we explored the predictive value of this data when strains were grown in the more physiologically relevant environments of urine and urothelial organoids. Resistant E. coli isolates were selected for following 24-h exposure to sub-inhibitory concentrations of AMC in either M9, ISO, or LB, followed by growth on LB agar containing AMC. No resistant colonies emerged following growth in M9, whereas resistant isolates were detected from cultures grown in ISO and LB. We observed both within and between media-type variability in the levels of resistance and fitness of the resistant mutants grown in LB. MICs and fitness of these resistant strains in different media (M9, ISO, LB, human urine, and urothelial organoids) showed considerable variation. Media can therefore have a direct effect on the isolation of mutants that confer resistance to AMC and these mutants can exhibit unpredictable MIC and fitness profiles under different growth conditions. This preliminary study highlights the risks in relying on a single culture protocol as a model system to predict the behavior and treatment response of bacteria in vivo and highlights the importance of developing comprehensive experimental designs to ensure effective translation of diagnostic procedures to successful clinical outcomes

وصف الملف: application/pdf

العلاقة: https://westminsterresearch.westminster.ac.uk/download/905d192e6e277361be7c9176ceff7c0b0aa6952243f81631e25a45883d68e7cf/1176605/fmicb-10-02001.pdfTest; https://doi.org/10.3389/fmicb.2019.02001Test; Alasdair T. M. Hubbard, Nazila Jafari, Nicholas Feasey, Jennifer L. Rohn and Adam P. Roberts 2019. Effect of Environment on the Evolutionary Trajectories and Growth Characteristics of Antibiotic-Resistant Escherichia coli Mutants. Frontiers in Microbiology. 10 2001. https://doi.org/10.3389/fmicb.2019.02001Test

الإتاحة: https://doi.org/10.3389/fmicb.2019.02001Test
https://westminsterresearch.westminster.ac.uk/item/w85ww/effect-of-environment-on-the-evolutionary-trajectories-and-growth-characteristics-of-antibiotic-resistant-escherichia-coli-mutantsTest
https://westminsterresearch.westminster.ac.uk/download/905d192e6e277361be7c9176ceff7c0b0aa6952243f81631e25a45883d68e7cf/1176605/fmicb-10-02001.pdfTest

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7

A pangenome analysis comparing and contrasting Salmonella enterica subspecies enterica and Salmonella enterica subspecies salamae isolated from households in Malawi.

المؤلفون: Catherine Wilson, Mathew A. Beale, Patrick Musicha, Yohane Diness, Oscar Kanjerwa, Nicholas Feasey, Eric Fèvre, Gina Pinchbeck, Nicholas Thomson

مصطلحات موضوعية: Salmonella, Pangenome, References

الوصف: References to published literature within the poster; A pangenome analysis comparing and contrasting Salmonella enterica subspecies enterica and Salmonella enterica subspecies salamae isolated from households in Malawi.

العلاقة: https://zenodo.org/record/7821047Test; https://doi.org/10.5281/zenodo.7821047Test; oai:zenodo.org:7821047

الإتاحة: https://doi.org/10.5281/zenodo.7821047Test
https://doi.org/10.5281/zenodo.7821046Test
https://zenodo.org/record/7821047Test

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8

A pangenome analysis comparing and contrasting Salmonella enterica subspecies enterica and Salmonella enterica subspecies salamae isolated from households in Malawi.

المؤلفون: Catherine N. Wilson, Mathew Beale, Patrick Musicha, Yohane Diness, Oscar Kanjerwa, Nicholas Feasey, Eric Fèvre, Gina Pinchbeck, Nicholas Thomson

مصطلحات موضوعية: Salmonella, pangenome, salamae, enterica

الوصف: Genomic Methods performed during the study.

العلاقة: https://zenodo.org/record/7809015Test; https://doi.org/10.5281/zenodo.7809015Test; oai:zenodo.org:7809015

الإتاحة: https://doi.org/10.5281/zenodo.7809015Test
https://doi.org/10.5281/zenodo.7809014Test
https://zenodo.org/record/7809015Test

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9

صورة

Genome-wide fitness analysis identifies genes required for in vitro growth and macrophage infection by African and global epidemic pathovariants of Salmonella enterica Enteritidis

المؤلفون: Wai Yee Fong, Rocío Canals, Alexander V. Predeus, Blanca Perez-Sepulveda, Nicolas Wenner, Lizeth Lacharme-Lora, Nicholas Feasey, Paul Wigley, Jay C. D. Hinton

مصطلحات موضوعية: Gene expression (incl. microarray and other genome-wide approaches), Bacteriology, Microbiology not elsewhere classified, Infectious diseases, Salmonella enterica serovar enteritidis, Transposon sequencing, Essential genes, Macrophage, Fitness

الوصف: Supplementary Material for 'Genome-wide fitness analysis identifies genes required for in vitro growth and macrophage infection by African and global epidemic pathovariants of Salmonella enterica Enteritidis’, as described in Microbial Genomics. Supplementary Figures S1 - S4 Supplementary Tables S1 - S7

العلاقة: https://figshare.com/articles/figure/Genome-wide_fitness_analysis_identifies_genes_required_for_in_vitro_growth_and_macrophage_infection_by_African_and_global_epidemic_pathovariants_of_em_Salmonella_enterica_em_Enteritidis/21720416Test

الإتاحة: https://doi.org/10.6084/m9.figshare.21720416.v1Test
https://figshare.com/articles/figure/Genome-wide_fitness_analysis_identifies_genes_required_for_in_vitro_growth_and_macrophage_infection_by_African_and_global_epidemic_pathovariants_of_em_Salmonella_enterica_em_Enteritidis/21720416Test

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10

Genomic analysis of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli colonising adults in Blantyre, Malawi reveals previously undescribed diversity

المؤلفون: Joseph Lewis, Madalitso Mphasa, Rachel Banda, Mathew A. Beala, Jane Mallewa, Catherine Anscombe, Allan Zuza, Adam P. Roberts, Eva Heinz, Nicholas R. Thomson, Nicholas Feasey

مصطلحات موضوعية: Microbiology not elsewhere classified, Clinical microbiology, Whole-genome sequencing, Africa south of the Sahara, Drug resistance, microbial, extended spectrum beta-lactamases

الوصف: Supplemetary material for 'Genomic analysis of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli colonising adults in Blantyre, Malawi reveals previously undescribed diversity', as described in Microbial Genomics. Accession numbers and metadata for isolates described in the manuscript: Genomic analysis of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli colonising adults in Blantyre, Malawi reveals previously undescribed diversity.

العلاقة: https://figshare.com/articles/dataset/Genomic_analysis_of_extended-spectrum_beta-lactamase_ESBL_producing_em_Escherichia_coli_em_colonising_adults_in_Blantyre_Malawi_reveals_previously_undescribed_diversity/22561738Test

الإتاحة: https://doi.org/10.6084/m9.figshare.22561738.v1Test
https://figshare.com/articles/dataset/Genomic_analysis_of_extended-spectrum_beta-lactamase_ESBL_producing_em_Escherichia_coli_em_colonising_adults_in_Blantyre_Malawi_reveals_previously_undescribed_diversity/22561738Test

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