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1دورية أكاديمية
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2دورية أكاديمية
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3دورية أكاديمية
المؤلفون: Patricia H. Alvarenga, Thiago Luiz Alves e Silva, Motoshi Suzuki, Glenn Nardone, Pedro Cecilio, Joel Vega-Rodriguez, Jose M.C. Ribeiro, John F. Andersen
مصطلحات موضوعية: Biochemistry, Microbiology, Physiology, Ecology, Immunology, Inorganic Chemistry, Biological Sciences not elsewhere classified, free quantification approaches, families classically described, applied mass spectrometry, aedes aegypti <, 2 %, respectively, comprehensive proteomics analysis, also highly abundant, 102 serine proteases, based absolute quantification, serine protease, comprehensive catalogue, also provides, transports nutrients, relative abundances, proteins belonging, physiological importance, mosquito physiology, male mosquitoes, immune response, future studies, future research, disease transmission
الوصف: In arthropods, hemolymph carries immune cells and solubilizes and transports nutrients, hormones, and other molecules that are involved in diverse physiological processes including immunity, metabolism, and reproduction. However, despite such physiological importance, little is known about its composition. We applied mass spectrometry-based label-free quantification approaches to study the proteome of hemolymph perfused from sugar-fed female and male Aedes aegypti mosquitoes. A total of 1403 proteins were identified, out of which 447 of them were predicted to be extracellular. In both sexes, almost half of these extracellular proteins were predicted to be involved in defense/immune response, and their relative abundances (based on their intensity-based absolute quantification, iBAQ) were 37.9 and 33.2%, respectively. Interestingly, among them, 102 serine proteases/serine protease-homologues were identified, with almost half of them containing CLIP regulatory domains. Moreover, proteins belonging to families classically described as chemoreceptors, such as odorant-binding proteins (OBPs) and chemosensory proteins (CSPs), were also highly abundant in the hemolymph of both sexes. Our data provide a comprehensive catalogue of A. aegypti hemolymph basal protein content, revealing numerous unexplored targets for future research on mosquito physiology and disease transmission. It also provides a reference for future studies on the effect of blood meal and infection on hemolymph composition.
الإتاحة: https://doi.org/10.1021/acs.jproteome.3c00918.s001Test
https://figshare.com/articles/journal_contribution/Comprehensive_Proteomics_Analysis_of_the_Hemolymph_Composition_of_Sugar-Fed_i_Aedes_aegypti_i_Female_and_Male_Mosquitoes/25342872Test -
4دورية أكاديمية
المؤلفون: Patricia H. Alvarenga, Thiago Luiz Alves e Silva, Motoshi Suzuki, Glenn Nardone, Pedro Cecilio, Joel Vega-Rodriguez, Jose M.C. Ribeiro, John F. Andersen
مصطلحات موضوعية: Biochemistry, Microbiology, Physiology, Ecology, Immunology, Inorganic Chemistry, Biological Sciences not elsewhere classified, free quantification approaches, families classically described, applied mass spectrometry, aedes aegypti <, 2 %, respectively, comprehensive proteomics analysis, also highly abundant, 102 serine proteases, based absolute quantification, serine protease, comprehensive catalogue, also provides, transports nutrients, relative abundances, proteins belonging, physiological importance, mosquito physiology, male mosquitoes, immune response, future studies, future research, disease transmission
الوصف: In arthropods, hemolymph carries immune cells and solubilizes and transports nutrients, hormones, and other molecules that are involved in diverse physiological processes including immunity, metabolism, and reproduction. However, despite such physiological importance, little is known about its composition. We applied mass spectrometry-based label-free quantification approaches to study the proteome of hemolymph perfused from sugar-fed female and male Aedes aegypti mosquitoes. A total of 1403 proteins were identified, out of which 447 of them were predicted to be extracellular. In both sexes, almost half of these extracellular proteins were predicted to be involved in defense/immune response, and their relative abundances (based on their intensity-based absolute quantification, iBAQ) were 37.9 and 33.2%, respectively. Interestingly, among them, 102 serine proteases/serine protease-homologues were identified, with almost half of them containing CLIP regulatory domains. Moreover, proteins belonging to families classically described as chemoreceptors, such as odorant-binding proteins (OBPs) and chemosensory proteins (CSPs), were also highly abundant in the hemolymph of both sexes. Our data provide a comprehensive catalogue of A. aegypti hemolymph basal protein content, revealing numerous unexplored targets for future research on mosquito physiology and disease transmission. It also provides a reference for future studies on the effect of blood meal and infection on hemolymph composition.
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5دورية أكاديمية
المؤلفون: Di Palma, L., Albo, G., Nardone, M., Pezzella, C., Musella, V., Belardo, M.
المصدر: Journal of Physics: Conference Series ; volume 2716, issue 1, page 012023 ; ISSN 1742-6588 1742-6596
الوصف: The T-WING project is a research project aimed at designing, manufacturing, qualifying and testing the new wing of Leonardo Next Generation Civil Tilt-Rotor technical demonstrator (NGCTR-TD), as part of Clean Aviation Fast Rotorcraft activities. The methodology proposed in this paper encompasses the development of high-fidelity modelling and simulation procedures in support to virtual certification methods for crashworthiness requirements of tiltrotors. Finite Element Analysis (FEA) of an aircraft drop test is a complex and detailed process that aims to simulate the structural behaviour during an impact or drop event. This type of analysis is critical for assessing the safety of an aircraft in emergency landing situations. Wing crashworthiness requirement is specific for tilt rotors: during a survivable crash event, the wing design must ensure a pre-defined rupture with the purpose of alleviating the inertial load acting on the fuselage to preserve the occupants from injuries and fire, guaranteeing the escape paths. Thus, the highly integrated T-WING wing box concept has been designed with the specific feature of frangible sections near the wing-fuselage intersection. The activation of fracture of the external semi-wings in correspondence of frangible section is triggered by the achievement of a well-defined crash vertical load factor. The objective of the methodology is to simulate the crash effects on the whole wing, using explicit non-linear and time-dependent FE analysis, to verify the wing spanwise placement of the frangible sections, the failure mode, the loads acting at the fuselage links, and the acceleration transmitted to the structure. This work is focused on a standalone analysis of the wing plus a lumped scheme of the fuselage, and it is part of a wider activity which will comprise, in the crash simulation, the most relevant vehicle systems (e.g. fuselage model). Moreover, this numerical activity has been compared with experimental results obtained on a different but similar structure, in ...
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6دورية أكاديمية
المؤلفون: Ciammella, Patrizia, Cozzi, Salvatore, Borghetti, Paolo, Galaverni, Marco, Nardone, Valerio, Ruggieri, Maria Paola, Sepulcri, Matteo, Scotti, Vieri, Bruni, Alessio, Zanelli, Francesca, Piro, Roberto, Tagliavini, Elena, Botti, Andrea, Iori, Federico, Alì, Emanuele, Bennati, Chiara, Tiseo, Marcello
المصدر: Frontiers in Oncology ; volume 14 ; ISSN 2234-943X
مصطلحات موضوعية: Cancer Research, Oncology
الوصف: Background Chemoradiation therapy (CRT) is the treatment of choice for locally advanced non-small cell lung cancer (LA-NSCLC). Several clinical trials that combine programmed cell death 1 (PD1) axis inhibitors with radiotherapy are in development for patients with LA-NSCLC. However, the effect of CRT on tumor cells programmed cell death ligand-1 (PD-L1) expression is unknown. Methods In this multicentric retrospective study, we analyzed paired NSCLC specimens that had been obtained pre- and post-CRT. PD-L1 expression on tumor cells was studied by immunohistochemistry. The purpose of this study was to evaluate the feasibility, risk of complications, and clinical relevance of performing re-biopsy after CRT in patients with PD-L1 negative LA-NSCLC. Results Overall, 31 patients from 6 centers with PD-L1 negative LA-NSCLC were analyzed. The percentage of tumor cells with PD-L1 expression significantly increased between pre- and post-CRT specimens in 14 patients (45%). Nine patients had unchanged PD-L1 expression after CRT, in five patients the rebiopsy material was insufficient for PD-L1 analysis and in two patients no tumor cells at rebiopsy were found. The post-rebiopsy complication rate was very low (6%). All patients with positive PD-L1 re-biopsy received Durvalumab maintenance after CRT, except one patient who had a long hospitalization for tuberculosis reactivation. Median PFS of patients with unchanged or increased PD-L1 expression was 10 and 16.9 months, respectively. Conclusion CRT administration can induce PD-L1 expression in a considerable fraction of PD-L1 negative patients at baseline, allowing them receiving the maintenance Durvalumab in Europe. Hence, after a definitive CRT, PD-L1 redetermination should be considered in patients with LA-NSCLC PD-L1 negative, to have a better selection of maintenance Durvalumab candidates.
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7مؤتمر
المؤلفون: Italiano, Cristina, Saker, Assia, Laganà, Massimo, Thomas, Minju, Hary, Benjamin, Nardone, Steve, Meynet, Nicolas, Pino, Lidia, Ramirez Santos, Alvaro, Vita, Antonio
الوصف: The present work deals with the design, manufacturing (by Laser Powder Bed Fusion), characterization, and catalytic activation (by a washcoating method) of Periodical Cellular Structures (POCS) 3D-printed in a cylindrical shape (Ø = 1cm, Length = 1.5 cm), in different materials (Al-, Cu-, Ni-alloys) and with various structural parameters (porosity, density, cell type and strut dimensions). The general aim is to intensify the ammonia synthesis throughout the development of structured catalysts with geometries that allow the integration with ammonia selective membranes in a membrane-based reactor to increase productivity at low temperatures (250-300°C) and pressure (20-25bar).
العلاقة: https://zenodo.org/communities/ambher-projectTest; https://zenodo.org/record/8362361Test; https://doi.org/10.5281/zenodo.8362361Test; oai:zenodo.org:8362361
الإتاحة: https://doi.org/10.5281/zenodo.8362361Test
https://doi.org/10.5281/zenodo.8362360Test
https://zenodo.org/record/8362361Test -
8كتاب
المؤلفون: Lunardi, G., Cassani, G., Gatti, M., Amadi, A., Marchiondelli, A., Malacalza, M., Vitiello, A., Verga, S., Nardone, C., Selleri, A.
المصدر: Expanding Underground - Knowledge and Passion to Make a Positive Impact on the World ; page 3429-3436 ; ISBN 9781003348030
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9دورية أكاديمية
المؤلفون: Camila Nardone, Julieta Maria Ariotti
المصدر: Cadernos Eletrônicos Direito Internacional sem Fronteiras 5(1) e20230115
الوصف: Este trabajo presenta un rastreo del tratamiento del concepto de Patrimonio Cultural Inmaterial (PCI) en la Organización de las Naciones Unidas para la Educación, la Ciencia y la Cultura (UNESCO). Metodológicamente se procurará analizar genealógicamente las fuentes documentales vinculadas al tema, relevadas del repositorio en línea en la UNESCO. La recuperación de los antecedentes sobre el Patrimonio Cultural Inmaterial puede contribuir a consolidar su consideración internacional protectora como bien común.
العلاقة: https://zenodo.org/communities/cedisfTest; https://zenodo.org/record/7746486Test; https://doi.org/10.5281/zenodo.7746486Test; oai:zenodo.org:7746486
الإتاحة: https://doi.org/10.5281/zenodo.7746486Test
https://doi.org/10.5281/zenodo.7746485Test
https://zenodo.org/record/7746486Test -
10دورية أكاديمية
المؤلفون: Yu-Chen Cheng, Shayna Stein, Agostina Nardone, Weihan Liu, Wen Ma, Gabriella Cohen, Cristina Guarducci, Thomas O. McDonald, Rinath Jeselsohn, Franziska Michor
مصطلحات موضوعية: Cancer, Tumor Biology, Molecular and Cellular Biology, Therapeutic Research and Development, Methods and Technology, Computational Methods, Mathematical modeling, Tumor Evolution, Breast Cancer, Small Molecule Agents, Endocrinology, Hormone signaling and inhibitors, Cell Cycle, CDKs and CDK inhibitors
الوصف: Supplementary Table S3 shows ratio of each schedule to the standard schedule at day 100 for simulated cell lines
الإتاحة: https://doi.org/10.1158/2767-9764.24574045.v1Test
https://figshare.com/articles/journal_contribution/Supplementary_Table_S3_from_Mathematical_Modeling_Identifies_Optimum_Palbociclib-fulvestrant_Dose_Administration_Schedules_for_the_Treatment_of_Patients_with_Estrogen_Receptor_positive_Breast_Cancer/24574045Test
