التفاصيل البيبلوغرافية
| العنوان: |
A functional group–guided approach to aptamers for small molecules |
| المؤلفون: |
Yang, Kyungae, Mitchell, Noelle M., Banerjee, Saswata, Cheng, Zhenzhuang, Taylor, Steven, Kostic, Aleksandra M., Wong, Isabel, Sajjath, Sairaj, Zhang, Yameng, Stevens, Jacob, Mohan, Sumit, Landry, Donald W., Worgall, Tilla S., Andrews, Anne M., Stojanovic, Milan N. |
| المصدر: |
Science, 380(6648), 942-948, (2023-06-02) |
| بيانات النشر: |
American Association for the Advancement of Science |
| سنة النشر: |
2023 |
| المجموعة: |
Caltech Authors (California Institute of Technology) |
| مصطلحات موضوعية: |
Multidisciplinary |
| الوصف: |
Aptameric receptors are important biosensor components, yet our ability to identify them depends on the target structures. We analyzed the contributions of individual functional groups on small molecules to binding within 27 target-aptamer pairs, identifying potential hindrances to receptor isolation—for example, negative cooperativity between sterically hindered functional groups. To increase the probability of aptamer isolation for important targets, such as leucine and voriconazole, for which multiple previous selection attempts failed, we designed tailored strategies focused on overcoming individual structural barriers to successful selections. This approach enables us to move beyond standardized protocols into functional group–guided searches, relying on sequences common to receptors for targets and their analogs to serve as anchors in regions of vast oligonucleotide spaces wherein useful reagents are likely to be found. ; © 2023 the authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original US government works. We thank B. Solaja, F. Katz, H. Hess, D. Stefanović, S. Rudchenko, A. K. Rinderspacher, C. Boyle, V. Cornish, and J. Loeb for their input during the writing of this manuscript. We thank S. Deng and A. K. Rinderspacher for helping Z. Cheng with the synthesis of analogs and handling of data. M.N.S. and K.Y. thank the Maple Syrup Urine Disease Family Group for inspiration and guidance and K. Strauss and K. Brigatti, Clinic for Special Children, Strasburg, Pennsylvania, for advice on testing and applications of leucine sensors. K.Y. thanks B.-T. Zhang for introducing her to hypernetwork theory, which led to insertion-reselection designs. M.N.S. dedicates the work to his teachers of organic chemistry, Y. Kishi and B. Solaja. The project was supported by the NIH (voriconazole, leucine, other small molecules, and general conceptualization of functional group–based approach, GM138843 to M.N.S.; neurotransmitters, DA045550 to A.M.A.; ... |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
https://doi.org/10.1126/science.abn9859Test; oai:authors.library.caltech.edu:1twdw-maf93; eprintid:122402; resolverid:CaltechAUTHORS:20230725-746162000.27 |
| DOI: |
10.1126/science.abn9859 |
| الإتاحة: |
https://doi.org/10.1126/science.abn9859Test |
| حقوق: |
info:eu-repo/semantics/openAccess ; Other |
| رقم الانضمام: |
edsbas.92D36714 |
| قاعدة البيانات: |
BASE |
