التفاصيل البيبلوغرافية
| العنوان: |
Genome-Editing-Mediated Restructuring of Tumor Immune Microenvironment for Prevention of Metastasis |
| المؤلفون: |
Dongyoon Kim (6449609), Yina Wu (398172), Gayong Shim (6842717), Yu-Kyoung Oh (297262) |
| سنة النشر: |
1753 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Molecular Biology, Immunology, Cancer, Space Science, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, preventing tumor recurrence, mature dendritic cells, activate immune cells, provided gene editing, upon nir irradiation, nir irradiation resulted, nir irradiation prevented, transforming growth factor, b16f10 lung metastasis, tumor immune microenvironment, b16f10 cancer cells, β gene editing, nir irradiation, tumor microenvironment, b16f10 tumor, b16f10 cells, metastasis modulating, tumor tissues, γ expression, vivo <, subsequent immunotherapy, primary tumors, mice treated |
| الوصف: |
Modulating the tumor immune microenvironment to activate immune cells has been investigated to convert cold to hot tumors. Here, we report that metal–lipid hybrid nanoparticle (MLN)-mediated gene editing of transforming growth factor-β (TGF-β) can restructure the tumor microenvironment to an “immune activated” state for subsequent immunotherapy. MLNs with cationic lipids and elemental metallic Au inside were designed to deliver plasmid DNA encoding TGF-β single guide RNA and Cas9 protein (pC9sTgf) and to convert near-infrared light (NIR) to heat. Upon NIR irradiation, MLNs induced photothermal anticancer effects and calreticulin exposure on B16F10 cancer cells. Lipoplexes of pC9sTgf and MLN (pC9sTgf@MLN) provided gene editing of B16F10 cells and in vivo tumor tissues. In mice treated with pC9sTgf@MLNs and NIR irradiation, the tumor microenvironment showed increases in mature dendritic cells, cytotoxic T cells, and interferon-γ expression. In B16F10 tumor-bearing mice, intratumoral injection of pC9sTgf@MLNs and NIR irradiation resulted in ablation of primary tumors. Application of pC9sTgf@MLNs and NIR irradiation prevented the growth of secondarily challenged B16F10 cells at distant sites and B16F10 lung metastasis. Combined TGF-β gene editing and phototherapy is herein supported as a modality for restructuring the tumor immune microenvironment and preventing tumor recurrence. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/journal_contribution/Genome-Editing-Mediated_Restructuring_of_Tumor_Immune_Microenvironment_for_Prevention_of_Metastasis/16915251Test |
| DOI: |
10.1021/acsnano.1c05420.s001 |
| الإتاحة: |
https://doi.org/10.1021/acsnano.1c05420.s001Test |
| حقوق: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.EB852F8B |
| قاعدة البيانات: |
BASE |
