المؤلفون: Alcala Zúñiga, Daphne, Espinoza-Torres, Erika, Das, Ranjit Kumar, Vargas, Magaly, Lopez-Juarez, Alejandro, Zarei, Masoud M., Baker, Kelsey, Gil, Mario, Rodrigo, Hansapani, Roy, Upal

المصدر: Health & Biomedical Sciences Faculty Publications and Presentations

مصطلحات موضوعية: Parkinson’s disease, DJ1, Enriched environment, SH-SY5Y, Cell model, Dopamine, The mouse model, Medical Neurobiology, Medicine and Health Sciences

الوصف: Parkinson’s disease (PD) is a neurological disorder that affects dopaminergic neurons. The lack of understanding of the underlying molecular mechanisms of PD pathology makes treating it a challenge. Several pieces of evidence support the protective role of enriched environment (EE) and exercise on dopaminergic neurons. The specific aspect(s) of neuroprotection after exposure to EE have not been identified. Therefore, we have investigated the protective role of EE on dopamine dysregulation and subsequent downregulation of DJ1 protein using in vitro and in vivo models of PD. Our study for the first time demonstrated that DJ1 expression has a direct correlation with dopamine downregulation in PD models and exposure to EE has a significant impact on improving the behavioral changes in PD mice. This research provides evidence that exercise in EE has a positive effect on PD without interfering with the current line of therapy.

وصف الملف: application/pdf

العلاقة: https://scholarworks.utrgv.edu/hbs_fac/152Test; https://scholarworks.utrgv.edu/context/hbs_fac/article/1152/viewcontent/s12035_024_03951_w.pdfTest

الإتاحة: https://doi.org/10.1007/s12035-024-03951-wTest
https://scholarworks.utrgv.edu/hbs_fac/152Test
https://scholarworks.utrgv.edu/context/hbs_fac/article/1152/viewcontent/s12035_024_03951_w.pdfTest

المؤلفون: Jasmine N. Tutol, Whitney S. Y. Ong, Shelby M. Phelps, Weicheng Peng, Helen Goenawan, Sheel C. Dodani

مصطلحات موضوعية: Biophysics, Biochemistry, Medicine, Cell Biology, Molecular Biology, Biotechnology, Immunology, Inorganic Chemistry, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, unique sensing mechanism, selection strategy resulted, living cells beyond, cell model overexpressing, three sensors display, genetically encoded sensors, fluorescent protein sensors, electrolytes ”, chloride, chloride binding pocket, anion walking screening, aequorea victoria <, imaging labile chloride, essential anion across, r195l ), chloron, chloron sensors, r195i ), fluorescence imaging

الوصف: Beyond its role as the “queen of electrolytes”, chloride can also serve as an allosteric regulator or even a signaling ion. To illuminate this essential anion across such a spectrum of biological processes, researchers have relied on fluorescence imaging with genetically encoded sensors. In large part, these have been derived from the green fluorescent protein found in the jellyfish Aequorea victoria . However, a standalone sensor with a turn-on intensiometric response at physiological pH has yet to be reported. Here, we address this technology gap by building on our discovery of the anion-sensitive fluorescent protein mNeonGreen (mNG). The targeted engineering of two non-coordinating residues, namely K143 and R195, in the chloride binding pocket of mNG coupled with an anion walking screening and selection strategy resulted in the ChlorON sensors: ChlorON-1 (K143W/R195L), ChlorON-2 (K143R/R195I), and ChlorON-3 (K143R/R195L). In vitro spectroscopy revealed that all three sensors display a robust turn-on fluorescence response to chloride (20- to 45-fold) across a wide range of affinities ( K d ≈ 30–285 mM). We further showcase how this unique sensing mechanism can be exploited to directly image labile chloride transport with spatial and temporal resolution in a cell model overexpressing the cystic fibrosis transmembrane conductance regulator. Building from this initial demonstration, we anticipate that the ChlorON technology will have broad utility, accelerating the path forward for fundamental and translational aspects of chloride biology.

العلاقة: https://figshare.com/articles/journal_contribution/Engineering_the_ChlorON_Series_Turn-On_Fluorescent_Protein_Sensors_for_Imaging_Labile_Chloride_in_Living_Cells/24857791Test

الإتاحة: https://doi.org/10.1021/acscentsci.3c01088.s001Test
https://figshare.com/articles/journal_contribution/Engineering_the_ChlorON_Series_Turn-On_Fluorescent_Protein_Sensors_for_Imaging_Labile_Chloride_in_Living_Cells/24857791Test

المؤلفون: Ruijuan Guan, Zefeng Kang, Ling Li, Xin Yan, Tianpeng Gao

مصطلحات موضوعية: Biochemistry, Medicine, Cell Biology, Genetics, Molecular Biology, Immunology, Developmental Biology, Cancer, Virology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, underwent physical examination, tnf )- α, inflammatory factors tnf, fetal bovine serum, 12 healthy subjects, il )- 1β, 96 controls ), cell counting kit, detected using enzyme, high glucose induced, high glucose activates, twelve patients diagnosed, calcium ion pathway, mutated snp sites, dr model cells, detected snp candidates, pik3ca regulates development, c allele increases, dr cell model

الوصف: Objective To explore their association with the development of diabetes retinopathy (DR), single nucleotide polymorphism (SNP) mutations were screened out by high-throughput sequencing and validated in patients diagnosed with DR. To understand the role of PIK3CA in the pathogenesis of DR and explore the relationship between PIK3CA,phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR),and DR, the effect of PIK3CA.rs17849079 mutation was investigated in a DR cell model. Methods Twelve patients diagnosed with DR at the Qinghai Provincial People’s Hospital from September 2020 to June 2021 were randomly selected as the case group, while 12 healthy subjects of similar age and gender who underwent physical examination in Qinghai Provincial People’s Hospital physical examination center during the same period were randomly selected as the control group. Blood samples (2 mL) were collected from both groups using EDTA anticoagulant blood collection vessels and frozen at −20°C for future analysis. SNP mutations were detected by high-throughput sequencing, and the shortlisted candidates were subjected by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. The detected SNP candidates were verified by expanding the sample size (first validation: 56 patients in the case group and 58 controls; second validation: 157 patients in the case group and 96 controls). A lentivirus vector carrying mutated or wild-type PIK3CA.rs17849079 was constructed. ARPE-19 cells were cultured in a medium supplemented with 10% fetal bovine serum (FBS) to establish a DR cell model. PIRES2-PIK3CA-MT and PIRES2-PIK3CA-WT vectors were transfected into DR model cells, which were categorized into control, mannitol, model, empty vector, PIK3CA wild-type, and PIK3CA mutant-type groups. Cell activity was detected by the cell counting kit (CCK)-8 assay, and cellular apoptosis was evaluated by flow cytometry. Glucose concentration and levels of cytokines tumor necrosis factor ...

العلاقة: https://figshare.com/articles/journal_contribution/CHB_frequencies_in_the_Hapmap_database_/24969744Test

الإتاحة: https://doi.org/10.1371/journal.pone.0295813.s002Test
https://figshare.com/articles/journal_contribution/CHB_frequencies_in_the_Hapmap_database_/24969744Test

المؤلفون: Ruijuan Guan, Zefeng Kang, Ling Li, Xin Yan, Tianpeng Gao

مصطلحات موضوعية: Biochemistry, Medicine, Cell Biology, Genetics, Molecular Biology, Immunology, Developmental Biology, Cancer, Virology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, underwent physical examination, tnf )- α, inflammatory factors tnf, fetal bovine serum, 12 healthy subjects, il )- 1β, 96 controls ), cell counting kit, detected using enzyme, high glucose induced, high glucose activates, twelve patients diagnosed, calcium ion pathway, mutated snp sites, dr model cells, detected snp candidates, pik3ca regulates development, c allele increases, dr cell model

الوصف: Objective To explore their association with the development of diabetes retinopathy (DR), single nucleotide polymorphism (SNP) mutations were screened out by high-throughput sequencing and validated in patients diagnosed with DR. To understand the role of PIK3CA in the pathogenesis of DR and explore the relationship between PIK3CA,phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR),and DR, the effect of PIK3CA.rs17849079 mutation was investigated in a DR cell model. Methods Twelve patients diagnosed with DR at the Qinghai Provincial People’s Hospital from September 2020 to June 2021 were randomly selected as the case group, while 12 healthy subjects of similar age and gender who underwent physical examination in Qinghai Provincial People’s Hospital physical examination center during the same period were randomly selected as the control group. Blood samples (2 mL) were collected from both groups using EDTA anticoagulant blood collection vessels and frozen at −20°C for future analysis. SNP mutations were detected by high-throughput sequencing, and the shortlisted candidates were subjected by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. The detected SNP candidates were verified by expanding the sample size (first validation: 56 patients in the case group and 58 controls; second validation: 157 patients in the case group and 96 controls). A lentivirus vector carrying mutated or wild-type PIK3CA.rs17849079 was constructed. ARPE-19 cells were cultured in a medium supplemented with 10% fetal bovine serum (FBS) to establish a DR cell model. PIRES2-PIK3CA-MT and PIRES2-PIK3CA-WT vectors were transfected into DR model cells, which were categorized into control, mannitol, model, empty vector, PIK3CA wild-type, and PIK3CA mutant-type groups. Cell activity was detected by the cell counting kit (CCK)-8 assay, and cellular apoptosis was evaluated by flow cytometry. Glucose concentration and levels of cytokines tumor necrosis factor ...

العلاقة: https://figshare.com/articles/journal_contribution/Primer_sequence_/24969741Test

الإتاحة: https://doi.org/10.1371/journal.pone.0295813.s001Test
https://figshare.com/articles/journal_contribution/Primer_sequence_/24969741Test

المؤلفون: Kai Zhang, Lina Zhang, Mengyu Han, Zhiping Pu, Jinjing Zhong, Yanmei Hou, Peng Zhou

مصطلحات موضوعية: Biophysics, Biochemistry, Medicine, Microbiology, Cell Biology, Immunology, Developmental Biology, Cancer, Mental Health, Hematology, Biological Sciences not elsewhere classified, mouse model due, mast cell degranulation, epithelial barrier integrity, cholera toxin adjuvant, 2h3 cell model, common food allergy, milk protein α, study found differences, activation cow ’, goat milk α, higher potential sensitization, milk allergy, goat α, study aimed, investigate differences, higher production, cow α, th2 responses, th2 cells

الوصف: Cow’s milk allergy is a common food allergy, with the milk protein α S1 -casein being a major allergen. This study aimed to investigate differences in sensitization between cow and goat α S1 -CN. Cow and goat α S1 -CN were labeled with fluorescent dyes and given to mice sensitized with cholera toxin adjuvant. Both proteins reached immune organs, suggesting no major difference in digestion. However, compared with goat α S1 -CN, cow α S1 -CN is more readily taken up by dendritic cells, inducing dendritic cell maturation. Furthermore, cow α S1 -CN can more effectively induce the generation of Th2 cells, leading to a higher production of specific IgE. In a Caco-2/RBL-2H3 cell model, cow α S1 -CN caused more mast cell degranulation and loss of epithelial barrier integrity than goat α S1 -CN. In summary, this study found differences in immune responses between cow and goat milk α S1 -CN. Cow α S1 -CN elicited stronger dendritic cell and Th2 responses, leading to increased mast cell degranulation.

العلاقة: https://figshare.com/articles/journal_contribution/Higher_Potential_Sensitization_of_Cow_sub_S1_sub_-Casein_over_Goat_sub_S1_sub_-Casein_in_a_Mouse_Model_due_to_Enhanced_Dendritic_Cell_Uptake_and_Activation/25077660Test

الإتاحة: https://doi.org/10.1021/acs.jafc.3c07688.s001Test
https://figshare.com/articles/journal_contribution/Higher_Potential_Sensitization_of_Cow_sub_S1_sub_-Casein_over_Goat_sub_S1_sub_-Casein_in_a_Mouse_Model_due_to_Enhanced_Dendritic_Cell_Uptake_and_Activation/25077660Test

المؤلفون: Yanan Li, Xinglong Zhu, Li Li, Chunjuan Bao, Qin Liu, Ning zhang, Ziyan He, Yi Ji, Ji Bao

مصطلحات موضوعية: Biophysics, Biochemistry, Medicine, Cell Biology, Molecular Biology, Pharmacology, Marine Biology, Cancer, Biological Sciences not elsewhere classified, Information Systems not elsewhere classified, Kaposiform hemangioendothelioma, 3D cell model, EOMA spheroid, VEGFC, Sirolimus

الوصف: Supplementary Material 1.

العلاقة: https://figshare.com/articles/journal_contribution/Additional_file_1_of_Construction_and_applications_of_the_EOMA_spheroid_model_of_Kaposiform_hemangioendothelioma/25414937Test

الإتاحة: https://doi.org/10.6084/m9.figshare.25414937.v1Test

المؤلفون: Kai Zhang, Lina Zhang, Mengyu Han, Zhiping Pu, Jinjing Zhong, Yanmei Hou, Peng Zhou

مصطلحات موضوعية: Biophysics, Biochemistry, Medicine, Microbiology, Cell Biology, Immunology, Developmental Biology, Cancer, Mental Health, Hematology, Biological Sciences not elsewhere classified, mouse model due, mast cell degranulation, epithelial barrier integrity, cholera toxin adjuvant, 2h3 cell model, common food allergy, milk protein α, study found differences, activation cow ’, goat milk α, higher potential sensitization, milk allergy, goat α, study aimed, investigate differences, higher production, cow α, th2 responses, th2 cells

الوصف: Cow’s milk allergy is a common food allergy, with the milk protein α S1 -casein being a major allergen. This study aimed to investigate differences in sensitization between cow and goat α S1 -CN. Cow and goat α S1 -CN were labeled with fluorescent dyes and given to mice sensitized with cholera toxin adjuvant. Both proteins reached immune organs, suggesting no major difference in digestion. However, compared with goat α S1 -CN, cow α S1 -CN is more readily taken up by dendritic cells, inducing dendritic cell maturation. Furthermore, cow α S1 -CN can more effectively induce the generation of Th2 cells, leading to a higher production of specific IgE. In a Caco-2/RBL-2H3 cell model, cow α S1 -CN caused more mast cell degranulation and loss of epithelial barrier integrity than goat α S1 -CN. In summary, this study found differences in immune responses between cow and goat milk α S1 -CN. Cow α S1 -CN elicited stronger dendritic cell and Th2 responses, leading to increased mast cell degranulation.

العلاقة: https://figshare.com/articles/journal_contribution/Higher_Potential_Sensitization_of_Cow_sub_S1_sub_-Casein_over_Goat_sub_S1_sub_-Casein_in_a_Mouse_Model_due_to_Enhanced_Dendritic_Cell_Uptake_and_Activation/25077660Test

الإتاحة: https://doi.org/10.1021/acs.jafc.3c07688.s001Test

المؤلفون: E Kodosaki, A Daniels-Morgan, N Hassan, R Webb, K Morris, CM Kelly

مصطلحات موضوعية: Biophysics, Cell Biology, Genetics, Neuroscience, Immunology, Developmental Biology, Cancer, Hematology, Biological Sciences not elsewhere classified, Neuroinflammation, microglia, Vicenin-2, cell model, differentiation

الوصف: Neuroinflammation is primarily characterised by activation of the brain’s resident macrophages – the microglia. However, other central nervous system (CNS) cells also contribute to this response, including the astrocytes and endothelial cells. In addition, there is infiltration into the CNS of peripherally derived immune cells. Together these cells mediate inflammation by the production of cytokines, chemokines, reactive oxygen species, and secondary messengers, and enacting of the appropriate response to those signals. However, deciphering the specific contributions of each cell type has been challenging. Studying CNS cell biology is often challenging, as the isolation of primary cells is not always feasible, and differentiation towards microglia-like cells is complex. Here, we demonstrate a novel method whereby THP-1 monocytic cells are differentiated into neural macrophage cells with microglia-like cell characteristics. The cells, designated mgTHP-1, show typical morphological and gene expression patterns of resident CNS macrophages and functionally respond to inflammatory stimuli by producing inflammatory cytokines. Furthermore, with the addition of Vicenin-2 (an anti-inflammatory flavonoid) such responses can be reversed. This novel cell model will allow further investigations, and hence insights, into the neuroinflammatory mechanisms associated with CNS diseases.

العلاقة: https://figshare.com/articles/journal_contribution/Development_and_characterisation_of_mgTHP-1_a_novel_i_in_vitro_i_model_for_neural_macrophages_with_microglial_characteristics/24521414Test

الإتاحة: https://doi.org/10.6084/m9.figshare.24521414.v1Test
https://figshare.com/articles/journal_contribution/Development_and_characterisation_of_mgTHP-1_a_novel_i_in_vitro_i_model_for_neural_macrophages_with_microglial_characteristics/24521414Test

المؤلفون: Shafia Nouman, Rosalind Silverman, Lorelei Silverman

مصطلحات موضوعية: Other health sciences not elsewhere classified, esophageal cancer cell model, biomarkers and mechanisms of action, proteomics, Squamous cell carcinoma, Adenocarcinoma, Ube3a gene

الوصف: Esophageal cancer is the eighth most commonly diagnosed cancer and is the sixth leading cause of cancer death worldwide.[1][2] The burden of this malignant tumor is significantly high in less developed regions, where almost 80% of all cases occur. Approximately 70% of cases occur in men, and there is a 2‐ to 5‐fold difference in incidence and mortality rates between the sexes.[2] In addition, esophageal cancer is higher in middle‐aged and elderly populations, and the chance of getting it increases with age.[3] The cancer burden of esophagus incidence and mortality is increasing rapidly worldwide, accompanied by global aging and population growth as well as the prevalence of associated risk factors such as tobacco and alcohol consumption, poor diet, lack of excise, and obesity.[1][4] Esophageal cancer is extremely malignant and prognosis is often poor.

العلاقة: https://figshare.com/articles/journal_contribution/_b_Protein-protein_Interactions_and_Novel_Biomarkers_of_Esophageal_Cancer_b_/24597426Test

الإتاحة: https://doi.org/10.6084/m9.figshare.24597426.v1Test
https://figshare.com/articles/journal_contribution/_b_Protein-protein_Interactions_and_Novel_Biomarkers_of_Esophageal_Cancer_b_/24597426Test

المؤلفون: Jiayi Sun, Biqi Tang, Chi-Tang Ho, Muwen Lu

مصطلحات موضوعية: Biochemistry, Cell Biology, Genetics, Molecular Biology, Physiology, Pharmacology, Cancer, Mental Health, Virology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, various pharmacological effects, resistant cell model, pungent alkaloid found, >- dependent manner, 98 ± 5, 76 ± 4, 47 ± 3, 43 ± 3, 36 ± 6, 19 ± 1, mitigated circadian disruption, hepg2 cells via, hepg2 cells piperine, circadian clock gene, pip alleviated glucosamine, hepg2 cells, circadian misalignment, piperine attenuates, pip alleviated

الوصف: Piperine (PIP), a pungent alkaloid found in black pepper, has various pharmacological effects by activating the transient receptor potential vanilloid 1 (TRPV1) receptor. In this study, the regulating effect of PIP on glucose metabolism and the underlying mechanism were examined using an insulin-resistant cell model. Results showed that PIP alleviated glucosamine (GlcN)-induced glucose metabolism disorder (from 59.19 ± 1.90 to 88.36 ± 6.57%), restored cellular redox balance (from 148.43 ± 3.52 to 110.47 ± 3.52%), improved mitochondrial function (from 63.76 ± 4.87 to 85.98 ± 5.12%), and mitigated circadian disruption in HepG2 cells via the mediation of circadian clock gene Bmal1 . After the knockdown of the Trpv1 gene, the modulating effect of PIP on Bmal1 -mediated glucose metabolism was weakened, indicating that PIP alleviated Bmal1 -involved insulin resistance and circadian misalignment in a Trpv1 -dependent manner in HepG2 cells.

العلاقة: https://figshare.com/articles/journal_contribution/Piperine_Attenuates_i_Bmal1_i_-Mediated_Glucose_Metabolism_Disorder_in_a_i_Trpv1_i_-Dependent_Manner_in_HepG2_Cells/24711709Test

الإتاحة: https://doi.org/10.1021/acs.jafc.3c06683.s001Test
https://figshare.com/articles/journal_contribution/Piperine_Attenuates_i_Bmal1_i_-Mediated_Glucose_Metabolism_Disorder_in_a_i_Trpv1_i_-Dependent_Manner_in_HepG2_Cells/24711709Test