دورية أكاديمية
A conserved megaprotein-based molecular bridge critical for lipid trafficking and cold resilience.
| العنوان: | A conserved megaprotein-based molecular bridge critical for lipid trafficking and cold resilience. |
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| المؤلفون: | Wang, Changnan, Wang, Bingying, Pandey, Taruna, Long, Yong, Zhang, Jianxiu, Oh, Fiona, Sima, Jessica, Guo, Ruyin, Liu, Yun, Zhang, Chao, Mukherjee, Shaeri, Bassik, Michael, Lin, Weichun, Deng, Huichao, Vale, Goncalo, McDonald, Jeffrey G, Shen, Kang, Ma, Dengke K |
| المصدر: | Nature communications, vol 13, iss 1 |
| بيانات النشر: | eScholarship, University of California |
| سنة النشر: | 2022 |
| المجموعة: | University of California: eScholarship |
| مصطلحات موضوعية: | Cell Membrane, Endoplasmic Reticulum, Animals, Zebrafish, Mammals, Humans, Caenorhabditis elegans, Phospholipids, Genetics, 2.1 Biological and endogenous factors, Aetiology, Generic health relevance |
| الوقت: | 6805 |
| الوصف: | Cells adapt to cold by increasing levels of unsaturated phospholipids and membrane fluidity through conserved homeostatic mechanisms. Here we report an exceptionally large and evolutionarily conserved protein LPD-3 in C. elegans that mediates lipid trafficking to confer cold resilience. We identify lpd-3 mutants in a mutagenesis screen for genetic suppressors of the lipid desaturase FAT-7. LPD-3 bridges the endoplasmic reticulum (ER) and plasma membranes (PM), forming a structurally predicted hydrophobic tunnel for lipid trafficking. lpd-3 mutants exhibit abnormal phospholipid distribution, diminished FAT-7 abundance, organismic vulnerability to cold, and are rescued by Lecithin comprising unsaturated phospholipids. Deficient lpd-3 homologues in Zebrafish and mammalian cells cause defects similar to those observed in C. elegans. As mutations in BLTP1, the human orthologue of lpd-3, cause Alkuraya-Kucinskas syndrome, LPD-3 family proteins may serve as evolutionarily conserved highway bridges critical for ER-associated non-vesicular lipid trafficking and resilience to cold stress in eukaryotic cells. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | qt1fj2h27h; https://escholarship.org/uc/item/1fj2h27hTest; https://escholarship.org/content/qt1fj2h27h/qt1fj2h27h.pdfTest |
| DOI: | 10.1038/s41467-022-34450-y |
| الإتاحة: | https://doi.org/10.1038/s41467-022-34450-yTest https://escholarship.org/uc/item/1fj2h27hTest https://escholarship.org/content/qt1fj2h27h/qt1fj2h27h.pdfTest |
| حقوق: | public |
| رقم الانضمام: | edsbas.FB6A2C49 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-022-34450-y |
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