التفاصيل البيبلوغرافية
| العنوان: |
Hira-Mediated H3.3 Incorporation Is Required for DNA Replication and Ribosomal RNA Transcription in the Mouse Zygote |
| المؤلفون: |
Lin, Chih-Jen, Koh, Fong Ming, Wong, Priscilla, Conti, Marco, Ramalho-Santos, Miguel |
| المصدر: |
Developmental Cell, vol 30, iss 3 |
| بيانات النشر: |
eScholarship, University of California |
| سنة النشر: |
2014 |
| المجموعة: |
University of California: eScholarship |
| مصطلحات موضوعية: |
Biological Sciences, Genetics, Human Genome, Stem Cell Research - Embryonic - Non-Human, Stem Cell Research, Generic health relevance, Animals, Cell Cycle Proteins, Chromatin, Chromatin Assembly and Disassembly, DNA Replication, Fertilization, Histone Chaperones, Histones, Male, Mice, Knockout, RNA, Ribosomal, Spermatozoa, Transcription Factors, Transcription, Genetic, Zygote, Medical and Health Sciences, Developmental Biology, Biochemistry and cell biology |
| جغرافية الموضوع: |
268 - 279 |
| الوصف: |
Extensive chromatin reprogramming occurs at fertilization and is thought to be under the control of maternal factors, but the underlying mechanisms remain poorly understood. We report that maternal Hira, a chaperone for the histone variant H3.3, is required for mouse development past the zygote stage. Male pronucleus formation is inhibited upon deletion of Hira due to a lack of nucleosome assembly in the sperm genome. Hira mutant oocytes are incapable of developing parthenogenetically, indicative of a role for Hira in the female genome. Both parental genomes show highly reduced levels of DNA replication and transcription in the mutants. It has long been thought that transcription is not required for zygote development. Surprisingly, we found that Hira/H3.3-dependent transcription of ribosomal RNA is required for first cleavage. Our results demonstrate that Hira-mediated H3.3 incorporation is essential for parental genome reprogramming and reveal an unexpected role for rRNA transcription in the mouse zygote. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
unknown |
| العلاقة: |
qt1n95t4pg; https://escholarship.org/uc/item/1n95t4pgTest |
| الإتاحة: |
https://escholarship.org/uc/item/1n95t4pgTest |
| حقوق: |
public |
| رقم الانضمام: |
edsbas.C17A94A1 |
| قاعدة البيانات: |
BASE |
