التفاصيل البيبلوغرافية
| العنوان: |
Genetic screen in Drosophila muscle identifies autophagy-mediated T-tubule remodeling and a Rab2 role in autophagy |
| المؤلفون: |
Fujita, Naonobu, Huang, Wilson, Lin, Tzu-han, Groulx, Jean-Francois, Jean, Steve, Nguyen, Jen, Kuchitsu, Yoshihiko, Koyama-Honda, Ikuko, Mizushima, Noboru, Fukuda, Mitsunori, Kiger, Amy A |
| بيانات النشر: |
eScholarship, University of California |
| سنة النشر: |
2017 |
| المجموعة: |
University of California: eScholarship |
| مصطلحات موضوعية: |
1.1 Normal biological development and functioning, Underpinning research, Animals, Autophagy, Drosophila Proteins, Drosophila melanogaster, Gene Expression Profiling, Gene Expression Regulation, Developmental, Lysosomes, Membrane Fusion, Morphogenesis, Muscles, Phagosomes, Protein Isoforms, Protein Transport, Qa-SNARE Proteins, R-SNARE Proteins, RNA, Small Interfering, SNARE Proteins, Signal Transduction, rab GTP-Binding Proteins, rab2 GTP-Binding Protein, rab7 GTP-Binding Proteins, Drosophila metamorphosis, Rab GTPase, T-tubule, autophagosome-lysosome fusion, cell biology |
| الوصف: |
Transverse (T)-tubules make-up a specialized network of tubulated muscle cell membranes involved in excitation-contraction coupling for power of contraction. Little is known about how T-tubules maintain highly organized structures and contacts throughout the contractile system despite the ongoing muscle remodeling that occurs with muscle atrophy, damage and aging. We uncovered an essential role for autophagy in T-tubule remodeling with genetic screens of a developmentally regulated remodeling program in Drosophila abdominal muscles. Here, we show that autophagy is both upregulated with and required for progression through T-tubule disassembly stages. Along with known mediators of autophagosome-lysosome fusion, our screens uncovered an unexpected shared role for Rab2 with a broadly conserved function in autophagic clearance. Rab2 localizes to autophagosomes and binds to HOPS complex members, suggesting a direct role in autophagosome tethering/fusion. Together, the high membrane flux with muscle remodeling permits unprecedented analysis both of T-tubule dynamics and fundamental trafficking mechanisms. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
unknown |
| العلاقة: |
qt6wm4b7r5; https://escholarship.org/uc/item/6wm4b7r5Test |
| الإتاحة: |
https://escholarship.org/uc/item/6wm4b7r5Test |
| حقوق: |
public |
| رقم الانضمام: |
edsbas.53E1C3B4 |
| قاعدة البيانات: |
BASE |
