التفاصيل البيبلوغرافية
| العنوان: |
Omega-3 polyunsaturated fatty acid-induced vasodilation in mouse aorta and mesenteric arteries is not mediated by ATP-sensitive potassium channels |
| المؤلفون: |
Bercea, Cristiana, Limbu, Roshan, Behnam, Kamila, Ng, Keat-Eng, Aziz, Qadeer, Tinker, Andrew, Tamagnini, Francesco, Cottrell, Graeme S, McNeish, Alister J |
| المساهمون: |
British Heart Foundation, National Institutes of Health |
| المصدر: |
Frontiers in Physiology ; volume 13 ; ISSN 1664-042X |
| بيانات النشر: |
Frontiers Media SA |
| سنة النشر: |
2022 |
| المجموعة: |
Frontiers (Publisher - via CrossRef) |
| مصطلحات موضوعية: |
Physiology (medical), Physiology |
| الوصف: |
There is strong evidence that the omega-3 polyunsaturated fatty acids (n-3 PUFAs) docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) have cardioprotective effects. n-3 PUFAs cause vasodilation in hypertensive patients, in part controlled by increased membrane conductance to potassium. As K ATP channels play a major role in vascular tone regulation and are involved in hypertension, we aimed to verify whether n-3 PUFA-mediated vasodilation involved the opening of K ATP channels. We used a murine model in which the K ATP channel pore subunit, Kir6.1, is deleted in vascular smooth muscle. The vasomotor response of preconstricted arteries to physiologically relevant concentrations of DHA and EPA was measured using wire myography, using the channel blocker PNU-37883A. The effect of n-3 PUFAs on potassium currents in wild-type native smooth muscle cells was investigated using whole-cell patch clamping. DHA and EPA induced vasodilation in mouse aorta and mesenteric arteries; relaxations in the aorta were sensitive to K ATP blockade with PNU-37883A. Endothelium removal didn’t affect relaxation to EPA and caused a small but significant inhibition of relaxation to DHA. In the knock-out model, relaxations to DHA and EPA were unaffected by channel knockdown but were still inhibited by PNU-37883A, indicating that the action of PNU-37883A on relaxation may not reflect inhibition of K ATP . In native aortic smooth muscle cells DHA failed to activate K ATP currents. We conclude that DHA and EPA cause vasodilation in mouse aorta and mesenteric arteries. Relaxations in blocker-treated arteries from knock-out mice demonstrate that K ATP channels are not involved in the n-3 PUFA-induced relaxation. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| DOI: |
10.3389/fphys.2022.1033216 |
| DOI: |
10.3389/fphys.2022.1033216/full |
| الإتاحة: |
https://doi.org/10.3389/fphys.2022.1033216Test |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.B86F39CF |
| قاعدة البيانات: |
BASE |
