التفاصيل البيبلوغرافية
| العنوان: |
Role of heat shock protein 47 in transdifferentiation of human tenon's fibroblasts to myofibroblasts. |
| المساهمون: |
Samin Hong, Kyoungsoo Park, Jin Hyoung Kim, Sueng-Han Han, Jong Bok Lee, Gong Je Seong, Park, Kyung Soo, Seong, Gong Je, Lee, Jong Bok, Han, Seung Han, Hong, Sa Min |
| المصدر: |
T201203818.pdf |
| سنة النشر: |
2012 |
| مصطلحات موضوعية: |
Blotting, Western, Cell Transdifferentiation/physiology, Cells, Cultured, Fibroblasts/cytology, Fibroblasts/metabolism, Gene Expression Regulation, HSP47 Heat-Shock Proteins/physiology, Humans, Myofibroblasts/cytology, Myofibroblasts/metabolism, RNA, Messenger/genetics, Real-Time Polymerase Chain Reaction, Fibroblast, Fibrosis, Heat shock protein, Myofibroblast, Transforming growth factor-棺 |
| الوصف: |
BACKGROUND: Heat shock protein 47 (Hsp47) is a well-known molecular chaperone in collagen synthesis and maturation. The aim of this study is to investigate its putative role in the transdifferentiation of Tenon's fibroblasts to myofibroblasts. METHODS: Primary cultured human Tenon's fibroblasts were exposed to transforming growth factor-棺1 (TGF-棺1) for up to 48 hours. The mRNA levels of Hsp47 and 慣 smooth muscle actin (慣SMA) were determined by quantitative real time RT-PCR. After delivery of small interfering RNA (siRNA) molecules targeting Hsp47 into the cells, the expression of Hsp47 and 慣SMA proteins was determined by western immunoblotting. RESULTS: TGF-棺1 increased the mRNA expressions of both Hsp47 and 慣SMA in human Tenon's fibroblasts, as determined by quantitative real time RT-PCR. However, it induced the protein expression of only 慣SMA but not Hsp47, as determined by western immunoblots. When siRNAs specific for Hsp47 were introduced into those cells, the TGF-棺1-induced expression of 慣SMA was significantly attenuated on western immunoblots; after 48 hours of exposure to TGF-棺1, the relative densities of immunobands were 11.58 for the TGF-棺1 only group and 2.75 for the siRNA treatment group, compared with the no treatment control group (p��<��0.001). CONCLUSIONS: Our data suggest that Hsp47 may be related to the TGF-棺1-induced transdifferentiation of human Tenon's fibroblasts to myofibroblasts. ; open |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| تدمد: |
1471-2415 |
| العلاقة: |
BMC OPHTHALMOLOGY; J00370; OAK-2012-02195; https://ir.ymlib.yonsei.ac.kr/handle/22282913/89795Test; T201203818; BMC OPHTHALMOLOGY, Vol.12 : 49, 2012 |
| الإتاحة: |
https://ir.ymlib.yonsei.ac.kr/handle/22282913/89795Test |
| حقوق: |
CC BY-NC-ND 2.0 KR ; https://creativecommons.org/licenses/by-nc-nd/2.0/krTest/ |
| رقم الانضمام: |
edsbas.2C164573 |
| قاعدة البيانات: |
BASE |
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