التفاصيل البيبلوغرافية
| العنوان: |
Endoribonuclease-Based Two-Component Repressor Systems for Tight Gene Expression Control in Plants |
| المؤلفون: |
Liang, Yan, Richardson, Sarah, Yan, Jingwei, Benites, Veronica T, Cheng-Yue, Clarabelle, Tran, Thu, Mortimer, Jenny, Mukhopadhyay, Aindrila, Keasling, Jay D, Scheller, Henrik V, Loqué, Dominique |
| المصدر: |
ACS Synthetic Biology, vol 6, iss 5 |
| بيانات النشر: |
eScholarship, University of California |
| سنة النشر: |
2017 |
| المجموعة: |
University of California: eScholarship |
| مصطلحات موضوعية: |
Biological Sciences, Bioinformatics and Computational Biology, Genetics, Bioengineering, Biotechnology, 5' Untranslated Regions, Bacterial Proteins, CRISPR-Associated Proteins, Endoribonucleases, Genetic Engineering, Models, Biological, Plant Proteins, Plants, Pseudomonas aeruginosa, Synthetic Biology, gene regulation, two-component repressor system, endoribonuclease, Csy4, CRISPR/Cas9, Medicinal and Biomolecular Chemistry, Biochemistry and Cell Biology, Biomedical Engineering |
| جغرافية الموضوع: |
806 - 816 |
| الوصف: |
Tight control and multifactorial regulation of gene expression are important challenges in genetic engineering and are critical for the development of regulatory circuits. Meeting these challenges will facilitate transgene expression regulation and support the fine-tuning of metabolic pathways to avoid the accumulation of undesired intermediates. By employing the endoribonuclease Csy4 and its recognition sequence from Pseudomonas aeruginosa and manipulating 5'UTR of mRNA, we developed a two-component expression-repression system to tightly control synthesis of transgene products. We demonstrated that this regulatory device was functional in monocotyledonous and dicotyledonous plant species, and showed that it can be used to repress transgene expression by >400-fold and to synchronize transgene repression. In addition to tissue-specific transgene repression, this system offers stimuli-dependent expression control. Using a bioinformatics approach, we identified 54 orthologous systems from various bacteria, and then validated in planta the activity for a few of those systems, demonstrating the potential diversity of such a two-component repressor system. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
qt8qv2n0dj; https://escholarship.org/uc/item/8qv2n0djTest |
| الإتاحة: |
https://escholarship.org/uc/item/8qv2n0djTest |
| حقوق: |
public |
| رقم الانضمام: |
edsbas.6B3FB3CB |
| قاعدة البيانات: |
BASE |
