دورية أكاديمية
Dynamical control enables the formation of demixed biomolecular condensates
| العنوان: | Dynamical control enables the formation of demixed biomolecular condensates |
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| المؤلفون: | Lin, Andrew Z, Ruff, Kiersten M, Dar, Furqan, Jalihal, Ameya, King, Matthew R, Lalmansingh, Jared M, Posey, Ammon E, Erkamp, Nadia A, Seim, Ian, Gladfelter, Amy S, Pappu, Rohit V |
| المصدر: | 2020-Current year OA Pubs |
| بيانات النشر: | Digital Commons@Becker |
| سنة النشر: | 2023 |
| المجموعة: | Washington University School of Medicine: Digital Commons@Becker |
| مصطلحات موضوعية: | Biomolecular Condensates, RNA, Thermodynamics, ICTS (Institute of Clinical and Translational Sciences), Medicine and Health Sciences |
| الوصف: | Cellular matter can be organized into compositionally distinct biomolecular condensates. For example, in Ashbya gossypii, the RNA-binding protein Whi3 forms distinct condensates with different RNA molecules. Using criteria derived from a physical framework for explaining how compositionally distinct condensates can form spontaneously via thermodynamic considerations, we find that condensates in vitro form mainly via heterotypic interactions in binary mixtures of Whi3 and RNA. However, within these condensates, RNA molecules become dynamically arrested. As a result, in ternary systems, simultaneous additions of Whi3 and pairs of distinct RNA molecules lead to well-mixed condensates, whereas delayed addition of an RNA component results in compositional distinctness. Therefore, compositional identities of condensates can be achieved via dynamical control, being driven, at least partially, by the dynamical arrest of RNA molecules. Finally, we show that synchronizing the production of different RNAs leads to more well-mixed, as opposed to compositionally distinct condensates in vivo. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | https://digitalcommons.wustl.edu/oa_4/2962Test; https://digitalcommons.wustl.edu/context/oa_4/article/3954/viewcontent/s41467_023_43489_4.pdfTest |
| DOI: | 10.1038/s41467-023-43489-4 |
| الإتاحة: | https://doi.org/10.1038/s41467-023-43489-4Test https://digitalcommons.wustl.edu/oa_4/2962Test https://digitalcommons.wustl.edu/context/oa_4/article/3954/viewcontent/s41467_023_43489_4.pdfTest |
| رقم الانضمام: | edsbas.27715B05 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-023-43489-4 |
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