دورية أكاديمية
An aqueous pomegranate seed extract ameliorates oxidative stress of human hepatoma HepG2 cells
| العنوان: | An aqueous pomegranate seed extract ameliorates oxidative stress of human hepatoma HepG2 cells |
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| المؤلفون: | Navarro, Marta, Amigo-Benavent, Miryam, Mesías, Marta, Baeza, Gema, Gökmen, Vural, Bravo, Laura, Morales, F. J. |
| بيانات النشر: | Wiley-VCH |
| سنة النشر: | 2014 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Glycation, Cytotoxicity, HepG2 cell model, Reactive oxygen species, Pomegranate seed |
| الوصف: | BACKGROUND: Aqueous pomegranate seed extract (PSE), a by-product of the pomegranate juice industry, was recently identified as a potential antiglycative ingredient. Ellagic acid was proposed as the major polyphenol responsible for the antiglycative activity as exerted in in vitro models. However, there is no information on safety aspects of this extract in biological systems before its application as ingredient. The cytotoxicity of PSE (1-100 μg mL-1) was evaluated by determining its effect on cell viability and redox status of cultured HepG2 cells. The protective effect of the PSE against oxidative stress induced by tert-butyl hydroperoxide (t-BOOH) was also investigated. RESULTS: No changes in cell integrity or intrinsic antioxidant status resulted from a direct treatment with aqueous PSE, even at high dosage. In addition, reactive oxygen species (ROS) induced by t-BOOH were reduced by 21% when cells were pretreated with 100 μg mL-1 of aqueous PSE at 180 min. The range of concentrations investigated was effective in decreasing the ROS formation but not in a dose-dependent manner. CONCLUSION: Aqueous pomegranate seed extract enhances human hepatoma cells integrity and resistance to cope with a stressful situation at concentration up to 100 μg mL-1. © 2013 Society of Chemical Industry. ; This work received grants from the Spanish Ministry of Science and Innovation under project NATURAGE (AGL2010-17779) and AGR1464-ANALISYC-II (CAM).The Project Consolider Ingenio CSD2007-00063 is also acknowledged. ; Peer Reviewed |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 1097-0010 |
| العلاقة: | Journal of the Science of Food and Agriculture 94: 1622- 1627 (2014); http://hdl.handle.net/10261/97454Test |
| DOI: | 10.1002/jsfa.6469 |
| الإتاحة: | https://doi.org/10.1002/jsfa.6469Test http://hdl.handle.net/10261/97454Test |
| حقوق: | open |
| رقم الانضمام: | edsbas.4CF69739 |
| قاعدة البيانات: | BASE |
| تدمد: | 10970010 |
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| DOI: | 10.1002/jsfa.6469 |