رسالة جامعية
Neuropeptide circuitries regulating food and water intake
| العنوان: | Neuropeptide circuitries regulating food and water intake |
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| المؤلفون: | Broberger, Christian |
| بيانات النشر: | Institutionen för neurovetenskap / Department of Neuroscience |
| سنة النشر: | 1999 |
| المجموعة: | Karolinska Institutet: Publications |
| مصطلحات موضوعية: | agouti gene-related protein, anorexia mutation, arcuate nucleus, cholecystokinin, cocaine- and amphetamine-regulated transcript, coexistence, confocal microscopy, dorsal root ganglion, feeding, hypocretin, immunohistochemistry, in situ hybridization, melanin-concentrating hormone, nodose ganglion, nucleus tractus solitaxii, orexin, paraventricular nucleus, satiety, sensory neurones, pro-opiomelanocortin, vagus nerve |
| الوصف: | An adequate supply of nutrients through food intake is critical for survival, as evidenced by disorders such as obesity and anorexia, which in the long run may be life-threatening. Feeding behaviour is ultimately controlled by interacting neuronal populations in the brain. The aim of this thesis was to investigate, mainly by histochemical methods, the neuronal pathways involved in this regulation. Firstly, we investigated the down-stream targets of neuropeptide Y (NPY)-expressing neurones in the hypothalamic arcuate nucleus, which appears to function as a critical center for receiving hormonal information of the metabolic state of the body and initiating food intake. By using as a marker agouti-gene-related protein (AGRP), which is solely expressed in the NPY neurones, the projections of this cell group were shown to innervate several nuclei extending from the olfactory nuclei to the nucleus tractus solitarii. Some of the hypothalamic target neurones of this projection were defined histochemically, and found to include neurones expressing cocaine- and amphetamine-regulate transcript (CART) in several nuclei, neurones expressing melaninconcentrating hormone or orexin/hypocretin in the lateral hypothalamic area, neurones expressing thyrotropin-releasing hormone in the paraventricular nucleus and neurones expressing pro-opiomelanocortin (POMC) in the arcuate nucleus. In the latter two populations, we also detected the expression of the NPY Y1 receptor, suggesting that NPY acts partly by inhibiting the activity of anorexigenic peptides. Secondly, in the mutant anorexia (anx/anx) mouse, which is characterized by decreased food intake, emaciation and premature death, we observed histochemical alterations in both the NPY and POMC arcuate cell populations. In the former, the levels of AGRP- and NPY-like immunoreactivites (-Lls) was increased in the cell bodies and decreased in terminals, whereas no change was observed in the respective mRNA levels; a pattern suggestive of accumulation. In contrast, markers of POMC ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| وصف الملف: | paper |
| اللغة: | unknown |
| ردمك: | 978-91-628-3625-2 91-628-3625-0 |
| العلاقة: | 91-628-3625-0; 19990604brob; http://hdl.handle.net/10616/44171Test |
| الإتاحة: | http://hdl.handle.net/10616/44171Test |
| رقم الانضمام: | edsbas.4152DB4B |
| قاعدة البيانات: | BASE |
| ردمك: | 9789162836252 9162836250 |
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