دورية أكاديمية
The expression profiles of the galectin gene family in primary and metastatic papillary thyroid carcinoma with particular emphasis on galectin-1 and galectin-3 expression
| العنوان: | The expression profiles of the galectin gene family in primary and metastatic papillary thyroid carcinoma with particular emphasis on galectin-1 and galectin-3 expression |
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| المؤلفون: | Salajegheh, Ali, Dolan-Evans, Elliot, Sullivan, Emily, Irani, Soussan, Rahman, Md, Vosgha, Haleh, Gopalan, Vinod, Smith, Robert, Lam, Alfred |
| المصدر: | Experimental and Molecular Pathology |
| بيانات النشر: | Academic Press |
| سنة النشر: | 2014 |
| المجموعة: | Queensland University of Technology: QUT ePrints |
| مصطلحات موضوعية: | Adult, Aged, Carcinoma/ genetics/pathology, Female, Galectin, Galectin 1, Galectin 1/ biosynthesis/genetics, Galectin 3, Galectin 3/ biosynthesis/genetics, Gene Expression Regulation, Humans, Immunohistochemistry, Lymph Nodes/metabolism/pathology, Lymphatic Metastasis/ genetics, Male, Messenger/biosynthesis, Middle Aged, Neoplastic, Papillary thyroid carcinoma, RNA, Thyroid Neoplasms/ genetics/pathology |
| الوصف: | INTRODUCTION: Galectin family members have been demonstrated to be abnormally expressed in cancer at the protein and mRNA level. This study investigated the levels of galectin proteins and mRNA expression in a large cohort of patients with papillary thyroid carcinoma and matched lymph node metastases with particular emphasis on galectin-1 and galectin-3. METHODS: mRNA expression of galectin family members (1, 2, 3, 4, 7, 8, 9, 10 and 12) were analysed by real-time polymerase chain reaction in 65 papillary thyroid carcinomas, 30 matched lymph nodes with metastatic papillary thyroid carcinoma and 5 non-cancer thyroid tissues. Galectin-1 and 3 protein expression was determined by immunohistochemistry in these samples. RESULTS: Significant expression differences in all tested galectin family members (1, 2, 3, 4, 7, 8, 9, 10 and 12) were noted for mRNA in papillary thyroid carcinomas, with and without lymph node metastasis. Galectin-1 protein was more strongly expressed than galectin-3 protein in papillary thyroid carcinoma. Galectin-1 protein was found to be overexpressed in 32% of primary papillary thyroid carcinomas. A majority of lymph nodes with metastatic papillary thyroid carcinoma (53%) had significantly increased expression of galectin-1 protein, as did 47% of primaries with metastases. Galectin-1 mRNA levels were decreased in the vast majority (94%) of primary thyroid carcinomas that did not have metastases present. Galectin-3 protein levels were noted to be overexpressed in 15% of primary papillary thyroid carcinomas. In primary papillary thyroid carcinoma with lymph node metastases, 32% had over expression of galectin-3 protein. Overexpression of galectin-3 mRNA was noted in 58% of papillary thyroid carcinomas and 64% of lymph nodes bearing metastatic papillary thyroid carcinoma. Also, primary papillary thyroid carcinoma with lymph node metastases had significantly higher expression of galectin-3 mRNA compared to those without lymph node metastases. CONCLUSION: Galectin family members show altered ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| العلاقة: | Salajegheh, Ali, Dolan-Evans, Elliot, Sullivan, Emily, Irani, Soussan, Rahman, Md, Vosgha, Haleh, Gopalan, Vinod, Smith, Robert, & Lam, Alfred (2014) The expression profiles of the galectin gene family in primary and metastatic papillary thyroid carcinoma with particular emphasis on galectin-1 and galectin-3 expression. Experimental and Molecular Pathology, 96(2), pp. 212-218.; https://eprints.qut.edu.au/74477Test/; Faculty of Health; Institute of Health and Biomedical Innovation |
| الإتاحة: | https://doi.org/10.1016/j.yexmp.2014.02.003Test https://eprints.qut.edu.au/74477Test/ |
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| رقم الانضمام: | edsbas.ACEB18B4 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |