المؤلفون: Lei Han, Xiaoyu Wei, Chuanyu Liu, Giacomo Volpe, Zhenkun Zhuang, Xuanxuan Zou, Zhifeng Wang, Taotao Pan, Yue Yuan, Xiao Zhang, Peng Fan, Pengcheng Guo, Yiwei Lai, Ying Lei, Xingyuan Liu, Feng Yu, Shuncheng Shangguan, Guangyao Lai, Qiuting Deng, Ya Liu, Liang Wu, Quan Shi, Hao Yu, Yunting Huang, Mengnan Cheng, Jiangshan Xu, Yang Liu, Mingyue Wang, Chunqing Wang, Yuanhang Zhang, Duo Xie, Yunzhi Yang, Yeya Yu, Huiwen Zheng, Yanrong Wei, Fubaoqian Huang, Junjie Lei, Waidong Huang, Zhiyong Zhu, Haorong Lu, Bo Wang, Xiaofeng Wei, Fengzhen Chen, Tao Yang, Wensi Du, Jing Chen, Shibo Xu, Juan An, Carl Ward, Zongren Wang, Zhong Pei, Chi-Wai Wong, Xiaolei Liu, Huafeng Zhang, Mingyuan Liu, Baoming Qin, Axel Schambach, Joan Isern, Liqiang Feng, Yan Liu, Xiangyu Guo, Zhen Liu, Qiang Sun, Patrick H. Maxwell, Nick Barker, Pura Muñoz-Cánoves, Ying Gu, Jan Mulder, Mathias Uhlen, Tao Tan, Shiping Liu, Huanming Yang, Jian Wang, Yong Hou, Xun Xu, Miguel A. Esteban, Longqi Liu

المصدر: Nature. 604:723-731

مصطلحات موضوعية: Macaca fascicularis, Multidisciplinary, Animals, Receptors, Virus, Cell Communication, Transcriptome, Wnt Signaling Pathway

الوصف: Studying tissue composition and function in non-human primates (NHPs) is crucial to understand the nature of our own species. Here we present a large-scale cell transcriptomic atlas that encompasses over 1 million cells from 45 tissues of the adult NHP Macaca fascicularis. This dataset provides a vast annotated resource to study a species phylogenetically close to humans. To demonstrate the utility of the atlas, we have reconstructed the cell-cell interaction networks that drive Wnt signalling across the body, mapped the distribution of receptors and co-receptors for viruses causing human infectious diseases, and intersected our data with human genetic disease orthologues to establish potential clinical associations. Our M. fascicularis cell atlas constitutes an essential reference for future studies in humans and NHPs. We thank W. Liu and L. Xu from the Huazhen Laboratory Animal Breeding Centre for helping in the collection of monkey tissues, D. Zhu and H. Li from the Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory) for technical help, G. Guo and H. Sun from Zhejiang University for providing HCL and MCA gene expression data matrices, G. Dong and C. Liu from BGI Research, and X. Zhang, P. Li and C. Qi from the Guangzhou Institutes of Biomedicine and Health for experimental advice or providing reagents. This work was supported by the Shenzhen Basic Research Project for Excellent Young Scholars (RCYX20200714114644191), Shenzhen Key Laboratory of Single-Cell Omics (ZDSYS20190902093613831), Shenzhen Bay Laboratory (SZBL2019062801012) and Guangdong Provincial Key Laboratory of Genome Read and Write (2017B030301011). In addition, L.L. was supported by the National Natural Science Foundation of China (31900466), Y. Hou was supported by the Natural Science Foundation of Guangdong Province (2018A030313379) and M.A.E. was supported by a Changbai Mountain Scholar award (419020201252), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA16030502), a Chinese Academy of Sciences–Japan Society for the Promotion of Science joint research project (GJHZ2093), the National Natural Science Foundation of China (92068106, U20A2015) and the Guangdong Basic and Applied Basic Research Foundation (2021B1515120075). M.L. was supported by the National Key Research and Development Program of China (2021YFC2600200). Sí

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::473adf27a5073ea8fa085eb52c85e80dTest
https://doi.org/10.1038/s41586-022-04587-3Test

المؤلفون: Jiangshan Xu, Shijie Hao, Quan Shi, Qiuting Deng, Yujia Jiang, Pengcheng Guo, Yue Yuan, Xuyang Shi, Shuncheng Shangguan, Huiwen Zheng, Guangyao Lai, Yaling Huang, Yang Wang, Yumo Song, Yang Liu, Liang Wu, Zhifeng Wang, Jiehui Cheng, Xiaoyu Wei, Mengnan Cheng, Yiwei Lai, Giacomo Volpe, Miguel A. Esteban, Yong Hou, Chuanyu Liu, Longqi Liu

المصدر: Xu, J, Hao, S, Shi, Q, Deng, Q, Jiang, Y, Guo, P, Yuan, Y, Shi, X, Shangguan, S, Zheng, H, Lai, G, Huang, Y, Wang, Y, Song, Y, Liu, Y, Wu, L, Wang, Z, Cheng, J, Wei, X, Cheng, M, Lai, Y, Volpe, G, Esteban, M A, Hou, Y, Liu, C & Liu, L 2022, ' Transcriptomic Profile of the Mouse Postnatal Liver Development by Single-Nucleus RNA Sequencing ', Frontiers in Cell and Developmental Biology, vol. 10, 833392 . https://doi.org/10.3389/fcell.2022.833392Test

مصطلحات موضوعية: CATENIN, BETA, INTRAHEPATIC BILE-DUCTS, Cell Biology, single-nucleus transcriptomics, hepatocyte maturation, MECHANISMS, ALPHA, ENDODERM, REGENERATION, bile acid synthesis, CELLS, postnatal liver development, liver zonation, SPECIFICATION, GENE-EXPRESSION, Developmental Biology

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d5ac3d315a5873e4b97e7db0c143bb4Test
https://doi.org/10.3389/fcell.2022.833392Test

المؤلفون: Yeya Yu, Xiaoyu Wei, Qiuting Deng, Qing Lan, Yiping Guo, Lei Han, Yue Yuan, Peng Fan, Peiying Wu, Shuncheng Shangguan, Yang Liu, Yiwei Lai, Giacomo Volpe, Miguel A. Esteban, Chuanyu Liu, Yong Hou, Longqi Liu

المصدر: Frontiers in Molecular Neuroscience
Frontiers in Molecular Neuroscience, Vol 14 (2021)

مصطلحات موضوعية: 0301 basic medicine, snATAC-seq, Cell type, Neurosciences. Biological psychiatry. Neuropsychiatry, Biology, Cell fate determination, 03 medical and health sciences, Cellular and Molecular Neuroscience, 0302 clinical medicine, Cortex (anatomy), Data Report, medicine, Molecular Biology, Gene, Transcription factor, transcription factor, regulatory element, Chromatin, 030104 developmental biology, medicine.anatomical_structure, chromatin accessibility, Regulatory sequence, rat cerebral cortex, Molecular Neuroscience, Neuroscience, Nucleus, 030217 neurology & neurosurgery, RC321-571

الوصف: Rats have been widely used as an experimental organism in psychological, pharmacological, and behavioral studies by modeling human diseases such as neurological disorders. It is critical to identify and characterize cell fate determinants and their regulatory mechanisms in single-cell resolutions across rat brain regions. Here, we applied droplet-based single-nucleus assay for transposase-accessible chromatin using sequencing (snATAC-seq) to systematically profile the single-cell chromatin accessibility across four dissected brain areas in adult Sprague–Dawley (SD) rats with a total of 59,023 single nuclei and identified 16 distinct cell types. Interestingly, we found that different cortex regions exhibit diversity in both cellular compositions and gene regulatory regions. Several cell-type-specific transcription factors (TFs), including SPI1, KLF4, KLF6, and NEUROD2, have been shown to play important roles during the pathogenesis of various neurological diseases, such as Alzheimer’s disease (AD), astrocytic gliomas, autism spectrum disorder (ASD), and intellectual disabilities. Therefore, our single-nucleus atlas of rat cortex could serve as an invaluable resource for dissecting the regulatory mechanisms underlying diverse cortex cell fates and further revealing the regulatory networks of neuropathogenesis.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f7e806d86e2ad00e70be9475c9f3742fTest
https://doi.org/10.3389/fnmol.2021.651355Test