نتائج البحث - "Photobacterium damselae"

1

FrpA is the outer membrane piscibactin transporter in Vibrio anguillarum: structural elements in synthetic piscibactin analogues required for transport

المؤلفون: Diana Martínez-Matamoros, Lucía Ageitos, Jaime Rodríguez, Marta A. Lages, Miguel Balado, Carlos Jiménez, Manuel L. Lemos, M. Carmen de la Fuente

المصدر: RUC. Repositorio da Universidade da Coruña
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الوصف: Financiado para publicación en acceso aberto: Universidade da Coruña/CISUG [Abstract] Piscibactin (Pcb) is a labile siderophore widespread among Vibrionaceae. Its production is a major virulence factor of some fish pathogens such as Photobacterium damselae subsp. piscicida and Vibrio anguillarum. Although FrpA was previously suggested as the putative outer membrane transporter (OMT) for ferri-piscibactin, its role in piscibactin uptake was never demonstrated. In this work, we generated mutants of V. anguillarum defective in FrpA and analyzed their ability to use piscibactin as iron source. The results showed that inactivation of frpA completely disables piscibactin utilization, and the original phenotype could be restored by gene complementation, confirming that FrpA is the OMT that mediates ferri-Pcb uptake. Additionally, the ability of several Pcb thiazole analogues, with different configurations at positions 9, 10, and 13, to be internalized through FrpA, was evaluated measuring their ability to promote growth under iron deficiency of several indicator strains. The results showed that while those analogues with a thiazole ring maintain almost the same activity as Pcb, the maintenance of the hydroxyl group present in natural piscibactin configuration at position C-13 is crucial for Fe³⁺ chelation and, in consequence, for the recognition of the ferri-siderophore by the cognate OMT. All these findings allowed us to propose a Pcb analogue as a good candidate to vectorize antimicrobial compounds, through the Trojan horse strategy, to develop novel compounds against bacterial fish diseases. This work was supported by grants RTI2018-093634-B-C21/C22 (AEI/FEDER, EU), cofunded by the FEDER Programme from the European Union, and by Grant PID2019-103891RJ-100 (AEI) from the Agency for Research (AEI) of Spain. Work in University of Santiago de Compostela and University of A Coruña was also supported by grants GRC2018/018 and GRC2018/039, respectively, from Xunta de Galicia and BLUEBIOLAB (0474_BLUEBIOLAB_1_E), Programme INTERREG V A of Spain-Portugal (POCTEP). L.A. thanks Xunta de Galicia (Spain) for a predoctoral fellowship (ED481A-2019/081) co-financed by European Social Fund (ESF). Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature Xunta de Galicia; GRC2018/018 Xunta de Galicia; GRC2018/039 Xunta de Galicia; ED481A-2019/081

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0fe79b801fbe60b68e3d99db7ce35a12Test
https://doi.org/10.1007/s00775-021-01916-1Test

2

Catastrophic mass mortalities caused by Photobacterium damselae affecting farmed marine fish from Deeba Triangle, Egypt

المؤلفون: Abdul Raouf M. Alkurdi, Mahmoud Abou-Okada, Mohamed Abdelsalam, Alaa Eldin Eissa, Rabia A. El Zlitne, Hassan Derwa, Abdelbary Prince

المصدر: Aquaculture Research. 52:4455-4466

مصطلحات موضوعية: Gilthead Seabream, Photobacterium damselae, Marine fish, Zoology, Aquatic Science, Biology, Photobacterium damselae subsp piscicida

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::33dd6bdcbdce5cc033a0628eda7d456fTest
https://doi.org/10.1111/are.15284Test

3

Vibriosis Incidents in Marine Finfish Farms: Prevalence, Diagnosis of Pathogens using 16S rRNA, Histopathology, and In Vitro Antibacterial Evaluation Against Isolated Vibrio spp using Antibiotics and Probiotics

المؤلفون: SangGuan You, Kandan Shanmugam, Babu Baskaran, Prabhu Narayanasamy Marimuthu, Mithun Raj, Mehdi Tabarsa, Sathiyaraj Ganesan, Palanisamy Subramanian, Anup Mandal

المصدر: Thalassas: An International Journal of Marine Sciences. 38:385-399

مصطلحات موضوعية: Gill, Veterinary medicine, biology, Fish farming, Outbreak, Aquatic Science, Oceanography, biology.organism_classification, 16S ribosomal RNA, Vibrio, law.invention, Probiotic, Photobacterium damselae, law, Bacteria

الوصف: Recently, in India, the marine finfish culture is expanding rapidly without adequate knowledge and research information on the coastal and marine finfish disease epidemics. Therefore, for the benefit of farmers, this study was designed to find the prevalence of Vibriosis outbreaks in marine finfish farms. In this study, 167 moribund fishes that show outward symptoms of lethargy, popeye, pale gills, skin erosions, abdominal edema, and hemorrhagic spots on different parts of the body were collected from 26 marine finfish farms (Lates calcarifer, Rachycentron canadum, and Trachinotus blochii) located in the states of Tamil Nadu, Andhra Pradesh, and Kerala, India. A total of 173 bacterial isolates were retrieved from all the fish samples, of which 144 isolates demonstrated a Vibrio-like character during biochemical and Vibriostatic 0/129 examinations. The phenotypic, 16s RNA gene sequencing and histopathological analysis confirm that the isolated bacterial strains were Vibriospecies. In the collected fish samples, the total prevalence of Vibrio species was 83.2% and the remaining 16.8% were other bacterial species. Among these bacteria, V. harveyi was the most predominant bacterium (34.1%) that cause infection in all the three fish species whereas Photobacterium damselae was found to be the least (2.9%) one. In the pathogenicity study, the highest mortality of 83% was observed after 168 h, when the seabass fingerling exposure to V. harveyi at 106 CFU/ml, and established similar clinical signs to the disease specimens of farmed fish. The in vitro antibacterial study confirms that the tested probiotic effectively inhibits the V. harveyi at 125 µl (108 CFU/ml). The above results inspire further in-depth studies on the probiotic efficacy to control the V. harveyi in finfish farms.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::a5b62947f6247ade2d2405e6394be8e2Test
https://doi.org/10.1007/s41208-021-00368-3Test

4

Evaluation of an oral DNA nanovaccine against photobacteriosis in Solea senegalensis

المؤلفون: C. Fernández-Díaz, Eugenia Zuasti, Elena Reales, Marian Ponce, V. Anguis

المصدر: Fish & Shellfish Immunology. 117:157-168

الوصف: Infectious diseases are one of the main causes of social and economical losses in world aquaculture. Senegalese sole (Solea senegalensis) is an important species for aquaculture in southern Europe, whose production is affected by the appearance of bacterial diseases such as photobacteriosis, a septicemia caused by Photobacterium damselae subsp. piscicida (Phdp). The aim of this study was to obtain an oral DNA nanovaccine and to evaluate its efficacy against Phdp in S. senegalensis juveniles. For this purpose, the amplified product corresponding to the protein inosine-5′-monophophate dehydrogenase (IMPDH) from Phdp, was cloned into the expression vector pcDNA™6.2/C-EmGFP-GW obtaining the DNA vaccine named as pPDPimpdh. The correct transcription and protein expression was verified at 48 h post tansfection in HEK293 cells. Chitosan nanoparticles (CS-TPP NPs) were prepared by ionotropic gelation and their features were appropriate for use as oral delivery system. Therefore, pPDPimpdh was protected with chitosan CS-TPP NPs throughout complex coacervation method giving as a result a DNA nanovaccine referred as CS-TPP+pPDPimpdh NPs. Sole juveniles were vaccinated orally with CS-TPP NPs, pPDPimpdh and CS-TPP+pPDPimpdh NPs followed by a challenge with Phdp at 30 days post vaccination (dpv). The relative percentage survival (RPS) for pPDPimpdh vaccinated groups was 6.25%, probably due to its degradation in the digestive tract. RPS value obtained for CS-TPP NPs and CS-TPP+pPDPimpdh NPs was 40% and antibodies were observed in both cases. However, a delay in mortality was observed in sole juveniles vaccinated orally with CS-TPP+pPDPimpdh NPs. In fact, an upregulation of tf, mhcII, cd8a and igm in the posterior gut and c3, hamp1, tf and cd4 in spleen was observed in juveniles vaccinated with CS-TPP+pPDPimpdh NPs. After challenge, a modulation of cd8a and cd4 expression levels in the posterior gut and c3, tf, lyg, cd4, igm and igt expression levels in spleen was observed. Moreover, the concentration of lysozyme in skin mucus significantly increased in fish vaccinated orally with CS-TPP+pPDPimpdh NPs at 11 dpc. These data indicate that oral vaccination with CS-TPP+pPDPimpdh NPs could be acting through the non-specific immune responses as well as the specific humoral and cell mediated immunity and provide the first step toward a development of an oral DNA nanovaccine against Phdp in sole.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f3d4f2ff9f98a73f05d1b5079e4e742Test
https://doi.org/10.1016/j.fsi.2021.07.023Test

5

Comparative analysis of spleen transcriptome of immune response in Sebastes schlegeli against Photobacterium damselae subsp. damselae infection

المؤلفون: Shi Linni, Xiaojun Rong, Yongxiang Yu, Yingeng Wang, Chunyuan Wang, Yongjie Li, Meijie Liao, Li Bin, Zheng Zhang

المصدر: Aquaculture Research. 53:232-242

مصطلحات موضوعية: Transcriptome, Immune system, medicine.anatomical_structure, biology, medicine, Spleen, Sebastes, Aquatic Science, Photobacterium damselae subsp damselae, biology.organism_classification, Microbiology

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::b5d4a4adef84ef48386f46df9291918bTest
https://doi.org/10.1111/are.15569Test

6

Monitoring Infection and Antibiotic Treatment in the Skin Microbiota of Farmed European Seabass (Dicentrarchus Labrax) Fingerlings

المؤلفون: Raquel Xavier, Ricardo Severino, Marcos Pérez-Losada, Daniela Rosado

المصدر: Microbial Ecology. 83:789-797

الوصف: The microbiota of fish skin, the primary barrier against disease, is highly dynamic and modulated by several factors. In fish aquaculture, disease outbreaks occur mainly during early-life stages, with associated high economic losses. Antibiotic treatments sometimes remain the best option to control bacterial diseases, despite many reported negative impacts of its use on fish and associated microbiota. Notwithstanding, studies monitoring the effects of disease and antibiotic treatment on the microbiota of fingerlings are scarce. We sequenced the bacterial 16S rRNA V4 gene region using a metabarcoding approach to assess the impact of a mixed infection with Photobacterium damselae ssp. piscicida and Vibrio harveyi and subsequent antibiotic treatment with flumequine, on the skin microbiota of farmed seabass (Dicentrarchus labrax) fingerlings. Both infection and antibiotic treatment led to a significant increase in bacterial diversity and core microbial communities and impacted microbiome structure. Dysbiosis was confirmed by changes in the abundance of potential pathogenic and opportunistic bacterial taxa. Skin bacterial metabolic function was also significantly affected by flumequine administration, suggesting a detriment to fish skin health. Our results add to an increasing body of literature, showing how fish microbiome response to infection and antibiotics cannot be easily predicted.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ccea009e410e36566d24fd6ea918c014Test
https://doi.org/10.1007/s00248-021-01795-8Test

7

Comparison of Intestine Microbiota Between Wild and Farmed Korean Rockfish, Sebastes schlegelii

المؤلفون: Kae Kyoung Kwon, Choong Hwan Noh, Hyun Sook Lee, Yun Jae Kim, Jae Kyu Lim, Jihyun Yu, Sung Gyun Kang, Jung-Hyun Lee, Mi-Jeong Park, Min Joo Kang

المصدر: Ocean Science Journal. 56:297-306

مصطلحات موضوعية: ved/biology, Fish farming, ved/biology.organism_classification_rank.species, Zoology, Biology, Oceanography, biology.organism_classification, Photobacterium, Rockfish, Photobacterium damselae, Aliivibrio, Sebastes schlegelii, Microbiome, Sebastes

الوصف: The Korean rockfish, Sebastes schlegeli, is most commonly farmed in sea cages along the coast of Korea; however, detailed information on intestinal microbiota regarding this fish is not readily available. In this study, comparison of the seasonal changes of microbial communities in the intestine between farmed and wild through the amplicon sequencing approach was conducted. The composition of major species in the intestine of this fish was very simple compared to that of other marine fish species, with members affiliated with the family Vibrionaceae hyper-dominating and comprising on average 97.6% of microbiota. However, the composition at the genus or species level and the pattern of seasonal changes of diversity indices showed significant differences between farmed and wild fish. In the farmed fish, Photobacterium phophoreum was most dominant throughout the year, accounting for 58.8% of the total. Aliivibrio fisherii and/or Aliivibrio finisterrensis also were dominant in the fall to winter but substituted by Photobacterium damselae during spring to summer. In the wild fish, on the other hand, opportunistic pathogens in the genera Aliivibrio or Vibrio were dominant in most of the samples. The analysis of shared species between gut microbiome, feed microbiota, and seawater microbiota indicated that the intestinal microbial diversity of farmed fish was affected more by microbiota of seawater than that of feed in spring and winter seasons. Additionally, the proportion of potential pathogenic Vibrio spp. in the gut showed a negative correlation with plasma glucose levels of the host. This study and following studies will be helpful in understanding the interaction between microbiome hosts and the development of techniques to enhance production of healthy Korean rockfish.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::48f97d273272d3af8352cb79872a7e3fTest
https://doi.org/10.1007/s12601-021-00022-2Test

8

Protective efficacy of four heat-shock proteins as recombinant vaccines against photobacteriosis in Asian seabass (Lates calcarifer)

المؤلفون: Pei-Chi Wang, Ta-Chih Cheng, Trung H.M. Pham, Shih-Chu Chen

المصدر: Fish & Shellfish Immunology. 111:179-188

الوصف: Photobacterium damselae subsp. piscicida (Phdp) is the causative agent of photobacteriosis in marine fish and is responsible for huge losses to marine aquaculture worldwide. Efforts have been made to develop a vaccine against this disease. Heat-shock proteins (HSPs) are a family of proteins that are ubiquitous in cellular life. Bacteria produce elevated levels of HSPs as a survival strategy when exposed to stressful environments in a host during infection. This group of proteins are also important antigens that can induce both humoral and cellular immune responses. In this study, four HSPs of Phdp, HSP90, HSP33, HSP70, and DnaJ, were selected for cloning and recombinant expression. Western blotting with rabbit anti-Phdp helped identify rHSP70 and rHSP33 as immunogenic proteins. Asian seabass (Lates calcarifer) immunised with rHSP90, rHSP33, rHSP70, and rDnaJ showed 48.28%, 62.07%, 51.72%, and 31.03% relative percent survival, respectively, after being challenged with Phdp strain AOD105021. High expression levels of immune-related genes and high antibody titres were observed in the rHSP33 group, and the sera of this group also exhibited a high level of bactericidal activity against Phdp. Collectively, our results suggest that HSP33 is a potential candidate for vaccine development against Phdp infection.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61d0295bfce4590368c43e140d292c7bTest
https://doi.org/10.1016/j.fsi.2021.02.002Test

9

Effects of a short pulse administration ofUlva rigidaon innate immune response and intestinal microbiota inSparus auratajuveniles

المؤلفون: Jorge García-Márquez, R. M. Rico, Félix L. Figueroa, Juan Miguel Mancera, Roberto Teofilo Abdala-Diaz, Juan Luis Gómez-Pinchetti, Miguel Ángel Moriñigo, E. Martinez-Manzanares, Francisco Javier Alarcón

المصدر: Aquaculture Research. 52:3038-3051

مصطلحات موضوعية: Immune system, Innate immune system, Pulse (signal processing), Aquatic Science, Biology, Photobacterium damselae subsp piscicida, Ulva rigida, Microbiology

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::5889fd41f6a5115e92eafd88193b15a1Test
https://doi.org/10.1111/are.15148Test

10

Isolation and Characterization of Fish-Gut Bacillus spp. as Source of Natural Antimicrobial Compounds to Fight Aquaculture Bacterial Diseases

المؤلفون: Paula Enes, Pedro Pousão-Ferreira, Maria José Saavedra, Aires Oliva-Teles, Russell Jerusik, Rafaela Santos, Cláudia R. Serra

المصدر: Marine Biotechnology. 23:276-293

الوصف: Aquaculture is responsible for more than 50% of global seafood consumption. Bacterial diseases are a major constraint to this sector and associated with misuse of antibiotics, pose serious threats to public health. Fish-symbionts, co-inhabitants of fish pathogens, might be a promising source of natural antimicrobial compounds (NACs) alternative to antibiotics, limiting bacterial diseases occurrence in aquafarms. In particular, sporeforming Bacillus spp. are known for their probiotic potential and production of NACs antagonistic of bacterial pathogens and are abundant in aquaculture fish guts. Harnessing the fish-gut microbial community potential, 172 sporeforming strains producing NACs were isolated from economically important aquaculture fish species, namely European seabass, gilthead seabream, and white seabream. We demonstrated that they possess anti-growth, anti-biofilm, or anti-quorum-sensing activities, to control bacterial infections and 52% of these isolates effectively antagonized important fish pathogens, including Aeromonas hydrophila, A. salmonicida, A. bivalvium, A. veronii, Vibrio anguillarum, V. harveyi, V. parahaemolyticus, V. vulnificus, Photobacterium damselae, Tenacibaculum maritimum, Edwardsiela tarda, and Shigella sonnei. By in vitro quantification of sporeformers' capacity to suppress growth and biofilm formation of fish pathogens, and by assessing their potential to interfere with pathogens communication, we identified three promising candidates to become probiotics or source of bioactive molecules to be used in aquaculture against bacterial aquaculture diseases.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a3ed7cc2013e2580291a5afb07021a9Test
https://doi.org/10.1007/s10126-021-10022-xTest

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