المؤلفون: Karima F. Mahrous, Heba A. M. Abd El -Kader, Mohamad A. Abdelhafez, Mohamad M. Aboelenin, Esraa A. Balabel, Dalia M. Mabrouk, Osama M. EL Malky, Mohamed S. Hassanane

المصدر: Journal of Genetic Engineering and Biotechnology. 20

مصطلحات موضوعية: Genetics, Biotechnology

الوصف: Background This study aimed to explore the association between polymorphisms in three genes: leptin (LEP), leptin receptor (LEPR), and BMP4, and incidence of repeat breeding in Egyptian buffaloes. Methods DNA was extracted from 160 female buffaloes, involving 108 fertile and 52 repeat breeders. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Sequence analysis and alignment were performed by employing NCBI/BLAST/blastn suite, to identify SNPs among different patterns and alleles. We utilized PredictSNP software to predict the non-synonymous SNPs influences on protein function. Moreover, the conservation score of the amino acids within the target proteins was computed by ConSurf server. Results The genotyping results showed that LEP and BMP4 genes were monomorphic (CC, GG) in all tested fertile and repeat breeder buffaloes. Leptin gene sequencing showed a non-synonymous C73T SNP, replacing R to C at position 25 within the leptin polypeptide (position 4 in the mature form; R4C) which is a neutral mutation, not affecting function or structure of LEP protein. For LEPR, one synonymous SNP (T102C) and two non-synonymous SNPs (A106G and C146A), triggering V967A and G954C replacements, respectively in LEPR protein. Moreover, they are neutral mutations. Sequencing results of BMP4 showed HinfI restriction site indicate fixed GG genotype (CC genotype in the anti-sense strand) in all sequenced samples. No SNPs were observed within the amplified region. Conclusion Genotyping and sequencing results of the surveyed three genes revealed that there is no association between these genes mutations and the incidence of repeat breeding in Egyptian buffaloes.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::0cbb512054456828ac4f3d0406c3139cTest
https://doi.org/10.1186/s43141-022-00397-2Test

المؤلفون: Karima F, Mahrous, Heba A M Abd, El-Kader, Mohamad A, Abdelhafez, Mohamad M, Aboelenin, Esraa A, Balabel, Dalia M, Mabrouk, Osama M, El Malky, Mohamed S, Hassanane

المصدر: Journal, genetic engineeringbiotechnologyReferences. 20(1)

الوصف: This study aimed to explore the association between polymorphisms in three genes: leptin (LEP), leptin receptor (LEPR), and BMP4, and incidence of repeat breeding in Egyptian buffaloes.DNA was extracted from 160 female buffaloes, involving 108 fertile and 52 repeat breeders. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Sequence analysis and alignment were performed by employing NCBI/BLAST/blastn suite, to identify SNPs among different patterns and alleles. We utilized PredictSNP software to predict the non-synonymous SNPs influences on protein function. Moreover, the conservation score of the amino acids within the target proteins was computed by ConSurf server.The genotyping results showed that LEP and BMP4 genes were monomorphic (CC, GG) in all tested fertile and repeat breeder buffaloes. Leptin gene sequencing showed a non-synonymous C73T SNP, replacing R to C at position 25 within the leptin polypeptide (position 4 in the mature form; R4C) which is a neutral mutation, not affecting function or structure of LEP protein. For LEPR, one synonymous SNP (T102C) and two non-synonymous SNPs (A106G and C146A), triggering V967A and G954C replacements, respectively in LEPR protein. Moreover, they are neutral mutations. Sequencing results of BMP4 showed HinfI restriction site indicate fixed GG genotype (CC genotype in the anti-sense strand) in all sequenced samples. No SNPs were observed within the amplified region.Genotyping and sequencing results of the surveyed three genes revealed that there is no association between these genes mutations and the incidence of repeat breeding in Egyptian buffaloes.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::82922eb754d07c76a6889ffc051baee2Test
https://pubmed.ncbi.nlm.nih.gov/35867170Test

المؤلفون: Alkhateib Y. Gaafar, Dalia M. Mabrouk, Mohamad M. Aboelenin, Mohamed S. Hassanane, Heba A.M. Abd El-Kader, Mahmoud A. Mahmoud, Wagdy K. B. Khalil, Karima F. Mahrous, Abdelgayed M. Younes

المصدر: Journal of Peptide Science. 26

مصطلحات موضوعية: food.ingredient, Microbial Sensitivity Tests, Biology, 010402 general chemistry, 01 natural sciences, Biochemistry, Fish Diseases, Nile tilapia, Minimum inhibitory concentration, food, Structural Biology, Drug Discovery, Gene expression, Animals, Molecular Biology, Pharmacology, chemistry.chemical_classification, 010405 organic chemistry, Aquaculture of tilapia, Organic Chemistry, Tilapia, Cichlids, General Medicine, Antimicrobial, biology.organism_classification, Immunohistochemistry, Molecular biology, Aeromonas hydrophila, Anti-Bacterial Agents, 0104 chemical sciences, Amino acid, chemistry, Molecular Medicine, Gram-Negative Bacterial Infections, Antimicrobial Cationic Peptides

الوصف: The antimicrobial activity of tilapia piscidin 3 (TP3) was determined in vitro against a locally isolated Aeromonas hydrophila. A 388 bp fragment was amplified from the TP3 cDNA and sequenced. The coding sequence (CDS) of TP3 was estimated to be 231 bp codes for 76 amino acids long and stop codon. In silico analysis was performed to detect both the signal peptide and the prodomain cleavage sites to follow the amino acids number 22 and 70, respectively. Based on this, a peptide 23 amino acids long with a remarkably high computed antimicrobial probability was synthesized and used in the subsequent experiments. The antimicrobial activity of TP3 was determined with minimum inhibitory concentration (MIC) and minim um bactericidal concentration (MBC) methods. TP3 exhibited relatively weak antimicrobial activities against the tested bacteria. A challenge experiment was then performed in Nile tilapia with low and high doses of A. hydrophila, followed by timely recognition; after 3, 6, 24 h, and 7 days of the specific TP3 gene expression, immunohistochemical localization was also performed. Histopathological examination revealed provoked inflammatory responses and congestion in the same organs of TP3 expression. Immunohistochemical localization showed that A. hydrophila induced tilapia fish to express TP3 after 24 h within the gills, intestine, hepatopancreas, spleen, and posterior kidney. In quantitative real time (RT)-polymerase chain reaction analysis, the high dose showed higher mRNA expression levels than the low dose, and its expression levels increased in the A. hydrophila-infected fish. It was therefore concluded that TP3 plays an essential role in fish immunity.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::14ff4451b653ebd91b7f4019f5fa4051Test
https://doi.org/10.1002/psc.3280Test

المؤلفون: Abdelgayed M. Younes, Mohamed S. Hassanane, Dalia M. Mabrouk, Mahmoud A. Mahmoud, Karima F. Mahrous, Alkhateib Y. Gaafar, Wagdy K. B. Khalil, Heba A.M. Abd El-Kader, Mohamad M. Aboelenin

المصدر: Developmental and comparative immunology. 112

مصطلحات موضوعية: 0301 basic medicine, Fish Proteins, Vibrio anguillarum, food.ingredient, Immunology, Pseudomonas fluorescens, Microbiology, 03 medical and health sciences, Nile tilapia, Fish Diseases, food, Gene expression, Animals, Streptococcus iniae, biology, Tilapia, 04 agricultural and veterinary sciences, Bacterial Infections, biology.organism_classification, Up-Regulation, Oreochromis, 030104 developmental biology, Aeromonas, Organ Specificity, 040102 fisheries, 0401 agriculture, forestry, and fisheries, Transcriptome, Developmental Biology, Antimicrobial Cationic Peptides

الوصف: The antimicrobial activity of tilapia piscidin 4 (TP4) was determined in vitro against four bacterial strains, Aeromonas hydrophilla, Pseudomonas fluorescens, Streptococcus iniae and Vibrio anguillarum. Nile tilapia were infected with low and high doses of the tested pathogens; after 3, 6, 24 h and 7 days of the specific TP4 gene expression, tissue immunolocalization was also performed. Histopathological examination revealed septicaemia and necrosis of hemopoietic tissue for all of the tested bacteria. Immunolocalization showed abundance in S. iniae-infected fish tissues. Quantitative RT-PCR analysis revealed that high doses raised mRNA expression levels compared to low doses and expression levels increased in the infected fish, particularly after 24 h, indicating that TP4 exerts potent bactericidal activity against some fish pathogens and plays an essential role in fish immunity.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8052f3995f13137e6e384a901f3cc97Test
https://pubmed.ncbi.nlm.nih.gov/32634526Test

المؤلفون: Mohamed S. Hassanane, Wagdy K. B. Khalil, Noha M. Osman, Karima F. Mahrous, Mohamad M. Aboelenin, Dalia M. Mabrouk, Heba A. M. Abd El Kader

المصدر: Bulletin of the National Research Centre, Vol 44, Iss 1, Pp 1-1 (2020)

مصطلحات موضوعية: 0106 biological sciences, 0303 health sciences, food.ingredient, Tilapia, Mistake, Biology, 01 natural sciences, 03 medical and health sciences, food, Expression analysis, General Earth and Planetary Sciences, lcsh:Q, lcsh:Science, Cartography, 030304 developmental biology, 010606 plant biology & botany, General Environmental Science

الوصف: After publication of the original article [1], we were notified that Figure 1 is incorrect. The corresponding author uploaded the wrong figure by mistake.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3b1bca4ebbe287b4dac00bd2585461eTest
https://doi.org/10.1186/s42269-020-00309-9Test

المؤلفون: G. Vaccarelli, Luca Giordano, Mohamed S. Hassanane, Hassan M. El Ashmaoui, Gianluca Tasco, Salvatrice Ciccarese, Fengtang Yang, Rita Casadio, Rachele Antonacci, Serafina Massari

المصدر: European Journal of Immunology. 42:3416-3428

مصطلحات موضوعية: Genetics, Germline mutation, biology, Immunology, T-cell receptor, biology.protein, Immunology and Allergy, Somatic hypermutation, Locus (genetics), Antibody, Framework region, Receptor, Gene

الوصف: In jawed vertebrates the V-(D)-J rearrangement is the main mechanism generating limitless variations of antigen-specific receptors, immunoglobulins (IGs), and T-cell receptors (TCRs) from few genes. Once the initial diversity is established in primary lymphoid organs, further diversification occurs in IGs by somatic hypermutation, a mechanism from which rearranged TCR genes were thought to be excluded. Here, we report the locus organization and expression of the T-cell receptor gamma (TCRG) genes in the Arabian camel (Camelus dromedarius). Expression data provide evidence that dromedary utilizes only two TCRG V-J genomic arrangements and, as expected, CDR3 contributes the major variability in the V domain. The data also suggest that diversity might be generated by mutation in the productively rearranged TCRGV genes. As for IG genes, the mutational target is biased toward G and C bases and (A/G/T)G(C/T)(A/T) motif (or DGYW). The replacement and synonymous substitutions (R/S) ratios in TCRGV regions are higher for CDR than for framework region, thus suggesting selection toward amino acid changes in CDR. Using the counterpart human TCR γδ receptor as a template, structural models computed adopting a comparative procedure show that nonconservative mutations contribute to diversity in CDR2 and at the γδ V domain interface.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::5a623839cd40157e805d9408e4eeb2bdTest
https://doi.org/10.1002/eji.201142176Test

المؤلفون: Mohamed S. Hassanane, Akmal A. El-Ghor, Hassan M. El Ashmaoui, Magda M. Noshy, Jehane I. Eid

المصدر: Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 696:160-166

مصطلحات موضوعية: Health, Toxicology and Mutagenesis, chemistry.chemical_element, Locus (genetics), Biology, Cadmium chloride, medicine.disease_cause, chemistry.chemical_compound, Genetics, medicine, Animals, Repeated sequence, Sequence Deletion, Cadmium, Chromosome Fragile Sites, Chromosomal fragile site, Microsatellite instability, medicine.disease, Molecular biology, Rats, chemistry, Microsatellite, Microsatellite Instability, Genotoxicity, Microsatellite Repeats, Mutagens

الوصف: Cadmium (Cd) is a non-essential element and is a widespread environmental pollutant. Exposure to cadmium can result in cytotoxic, carcinogenic and mutagenic effects. Mutagenesis is an indicative of genetic instability and can be assayed using microsatellites instability. The aim of the present study is to investigate; based on the rat model, the effects of oral acute (single 8.8 mg/kg BW, 1/10 LD 50 ) and sub-chronic (2.93 mg/kg BW, 1/30 LD 50 , for 4 weeks) doses of cadmium chloride on microsatellite instability at D6mit3, D9mit2 and D15Mgh1 loci, which are located in three different common fragile sites (6q21, 9q32-q33 and 15p14, respectively), within rat genome. In the acute study, no microsatellite instability (MSI) was observed in all the three tested loci (D6mit3, D9mit2 and D15Mgh1). In the sub-chronic study, the MSI were observed in the three studied loci and was in the form of deletion of 2–3 bp or addition of 3–6 bp. These finding may indicate the sensitivity of microsatellite sequences located at the fragile sites and the sensitivity of the simple sequence repeats (SSRs) assay for the detection of small variations in DNA sequence. However, additional chronic toxicological trials are needed in order to assess genotoxic effects of chronic exposure to Cd.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6093427cd3a9d0bb76d9337d0ee8a3c8Test
https://doi.org/10.1016/j.mrgentox.2010.01.007Test

المؤلفون: Mohamed S. Hassanane, El-Sayed A.M. Abd Alla, Aida I. El-Makawy

المصدر: Reproduction, nutrition, development. 41(1)

مصطلحات موضوعية: Male, Embryology, Ratón, Medicine (miscellaneous), Mitosis, Biology, medicine.disease_cause, Andrology, chemistry.chemical_compound, Mice, Pregnancy, Spermatocytes, medicine, Animals, Estrogens, Non-Steroidal, Mycotoxin, Zearalenone, Genetics, Chromosome Aberrations, Toxin, Embryo, Embryo, Mammalian, Teratology, Teratogens, Reproductive Medicine, chemistry, Toxicity, Animal Science and Zoology, Female, Genotoxicity, Developmental Biology, Food Science

الوصف: Genotoxic effects of the mycotoxin Zearalenone (ZEN) were evaluated on albino mice. The investigation was assessed using 4 criteria: chromosome aberrations in bone marrow and sper- matocytes of adult male mice; chromosome analysis and teratological effects of mice embryos. Zear- alenone was administrated to both adult males and pregnant females with 2 doses level (5 μg.kg -1 and 10 μg.kg -1 ZEN). Zearalenone was found to reduce the mitotic activity in treated males and the embryos proving that it is a cytotoxic substance. In treated males and females, it induced some chro- mosome abnormalities with no significant increase over the control at the doses investigated, except for some few figures. Similar results were observed for the teratological study. The results in general could consider zearalenone as a toxic mycotoxin for both adult animals and embryos. It is highly rec- ommended that a great attention should be paid towards the toxicity of zearalenone to mono-gastric animals and human, especially it contaminate corn that is widely used in human and animal feeding. genotoxicity / zearalenone / mice / embryo / chromosome / spermatocyte

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::daceaf89756b12191921956b399651e5Test
https://pubmed.ncbi.nlm.nih.gov/11368247Test