المؤلفون: Ekaterina R. Vasilevskaya, Andrei Kulikovskii, Elena Kotenkova, Irina M. Chernukha, L. V. Fedulova, Nadezhda Kupaeva

المصدر: Saudi Journal of Biological Sciences
Saudi Journal of Biological Sciences, Vol 28, Iss 5, Pp 2877-2885 (2021)

مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, Antioxidant, Oxygen radical absorbance capacity, QH301-705.5, GSH, reduced glutathione, ORAC, oxygen radical absorbance capacity, medicine.medical_treatment, IICI, integral indicators of chronic intoxication, 01 natural sciences, Husk, Superoxide dismutase, 03 medical and health sciences, chemistry.chemical_compound, ROS, reactive oxygen species, FR, free radical, TAC, total antioxidant capacity, SOD, superoxide dismutase, Vegetable waste, TBARS, thiobarbituric acid reactive substances, medicine, Food science, Biology (General), Antioxidant system, AOS, antioxidant system, Onion husk, MDA, malondialdehyde, HAT, hydrogen atom transfer, HPLC-MS, high performance liquid chromatography–mass spectrometry, biology, Chemistry, biology.organism_classification, Plant antioxidants, Ageing, 030104 developmental biology, Oxidative stress, Catalase, FRAP, fFerric reducing antioxidant power, biology.protein, Allium, Original Article, SET, single electron transfer, Trolox, CAT, catalase, GC–MS, gas chromatography–mass spectrometry, OHE, onion husk ethanolic extract, General Agricultural and Biological Sciences, Quercetin, 010606 plant biology & botany

الوصف: The role of natural antioxidants in preventing of age-relating diseases is evident. The vegetable industry generates a large amount of waste, which is a good source of antioxidants. The aim of the study was the investigation of the antioxidant effect of long-term consumption of ethanolic yellow onion husk extract in ageing laboratory rodents. Twenty male Wistar albino rats were divided randomly into two groups (n = 10): a control group and an experimental group that received ethanolic yellow onion husk extract (2 mL/rat diluted with distilled water; activity of 4.44 µmol-equiv. quercetin) for 188 days. Oxygen radical absorbance capacity and ferric reducing antioxidant power assays were used to determine the total antioxidant capacity of the extract, which amounted to 941.4 ± 32.7 µmol equiv. Trolox/g raw material and 167.4 ± 16.4 µmol-equiv. quercetin/g raw material, respectively. Oral intake of the onion husk extract affected the indicators of the antioxidant system of the liver and the brain but not of the blood and plasma, mainly due to elevations in the activity of catalase and superoxide dismutase in the liver by 44.4% and 79.1%, respectively, and in the brain by three-fold and 79.1%, respectively. The availability, cheapness and high antioxidant potential of onion waste qualifies it a good source of functional ingredients and bioactive substances applicable in the food and pharmaceutical industries.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::20ced9aff63bc8ff82a24da0f9e4bfefTest
https://doi.org/10.1016/j.sjbs.2021.02.020Test

المؤلفون: Fatimah O. Al-Otibi, Raedah I. Alharbi, Kahkashan Perveen, Manal A. Al-Mosa, Najat A. Bokhari, Gadah Albasher, Noura A. Al-Saif, Rana M. Al-Otaibi

المصدر: Saudi Journal of Biological Sciences, Vol 28, Iss 4, Pp 2229-2235 (2021)
Saudi Journal of Biological Sciences

مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, SPR, Surface Plasmon Resonance, Nanoparticle, Malva parviflora, 01 natural sciences, Alternaria alternata, Article, Silver nanoparticle, FE-SEM, Field Emission Scanning Electron Microscopy, 03 medical and health sciences, AgNPs, silver nanoparticles, Fusarium oxysporum, LEMP, leaf extract of M. parviflora, Zeta potential, Antifungal activity, FE-SEM, lcsh:QH301-705.5, Mycelium, FT-IR, Fourier-Transform Infrared Spectroscopy, biology, Chemistry, DLS, Dynamic Light Scattering, PDI, Polydispersity Index, biology.organism_classification, FT-IR, GC/MS, Gas Chromatography/Mass Spectrometry, 030104 developmental biology, lcsh:Biology (General), Helminthosporium rostratum, Silver nanoparticles, General Agricultural and Biological Sciences, Fusarium solani, 010606 plant biology & botany, Nuclear chemistry

الوصف: Cheeseweed mallow (Malva parviflora L.) was used to biosynthesize silver nanoparticles. The biosynthesized silver nanoparticles were classified by UV–vis Spectroscopy and Fourier-Transform Infrared Spectroscopy (FT-IR). The shape and size distribution were visualized by Transmission Electron Microscopy (TEM), Field Emission Scanning Electron Microscopy (FE-SEM), and Zeta potential analysis. The chemical composition of M. parviflora leaf extract was identified by Gas Chromatography and Mass Spectroscopy (GC/MS). Finally, in vitro antifungal assay was done to assess the potential of biosynthesized silver nanoparticles and crude leaf extract of M. parviflora for inhibiting the mycelial growth of phytopathogenic fungi. The UV–vis analysis manifests the formation of silver nanoparticles. FTIR analysis established that chemicals of the leaf extract stabilized the biosynthesized silver nanoparticles by binding with the free silver ions. The TEM, FE-SEM and zeta potential analyzer confirmed that the biosynthesized silver nanoparticles were mostly spherical with an average diameter of 50.6 nm. The biosynthesized silver nanoparticles and leaf extract of M. parviflora effectively mitigate the mycelial growth of Helminthosporium rostratum, Fusarium solani, Fusarium oxysporum, and Alternaria alternata. The maximum reduction in mycelial growth by biosynthesized nanoparticles was observed against H. rostratum (88.6%). Whereas, the leaf extract of M. parviflora was most effective against F. solani (65.3%). Thus, the biosynthesis of nanoparticle assisted by M. parviflora is a feasible and eco-friendly method for the synthesis of silver nanoparticles. Further the silver nanoparticles and leaf extract of M. parviflora could be explored for the development of the fungicide.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8afee26a5133c3436edc88298325c6f6Test
https://doi.org/10.1016/j.sjbs.2021.01.012Test

المؤلفون: Matthew S. Brook, Daniel J. Wilkinson, Kenneth Smith

المصدر: Clinical Nutrition Open Science, Vol 36, Iss, Pp 111-125 (2021)
Clinical Nutrition Open Science

مصطلحات موضوعية: A-V, Arterial Venous, Protein turnover, FBR, Fractional Breakdown Rate, Endocrinology, Diabetes and Metabolism, FSR, Fractional Synthesis Rate, Stable isotope tracers, Protein metabolism, Human metabolism, Computational biology, Biology, chemistry.chemical_compound, MPS, Muscle Protein Synthesis, Ra, Rate of Appearance, TX341-641, Nutrition and Dietetics, Nutrition. Foods and food supply, Stable isotope ratio, GC-MS, Gas Chromatography Mass Spectrometry, AP(E), Atom percent (excess), chemistry, LC-MS, Liquid Chromatography Mass Spectrometry, Muscle, Original Article, Rd, Rate of Disappearance, Whole body, AA, Amino Acids

الوصف: Summary: The key to understanding the mechanisms regulating disease stems from the ability to accurately quantify the dynamic nature of the metabolism underlying the physiological and pathological changes occurring as a result of the disease. Stable isotope tracer technologies have been at the forefront of this for almost 80 years now, and through a combination of both intense theoretical and technological development over these decades, it is now possible to utilise stable isotope tracers to investigate the complexities of in vivo human metabolism from a whole body perspective, down to the regulation of sub-nanometer cellular components (i.e organelles, nucleotides and individual proteins). This review therefore aims to highlight; 1) the advances made in these stable isotope tracer approaches – with special reference given to their role in understanding the nutritional regulation of protein metabolism, 2) some considerations required for the appropriate application of these stable isotope techniques to study protein metabolism, 3) and finally how new stable isotopes approaches and instrument/technical developments will help to deliver greater clinical insight in the near future.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae670894e656ca1db19fe9129802d6edTest
https://doi.org/10.1016/j.nutos.2021.02.005Test

المؤلفون: Kaushalya Amunugama, Matthew J. Jellinek, Megan P. Kilroy, Carolyn J. Albert, Valerio Rasi, Daniel F. Hoft, Michael G.S. Shashaty, Nuala J. Meyer, David A. Ford

المصدر: Redox Biology
Redox Biology, Vol 48, Iss, Pp 102208-(2021)

مصطلحات موضوعية: LPE, lysophosphatidylethanolamine, PMA, phorbol 12-myristate 13-acetate, Medicine (General), QH301-705.5, Clinical Biochemistry, Plasmalogens, HOCl, hypochlorous acid, MPO, myeloperoxidase, Biochemistry, PC, choline glycerophospholipid, Chlorolipids, VLC, very long chain, R5-920, 2-ClFA, 2-chlorofatty acid, Sepsis, HCl, hydrochloric acid, ESI MS/MS, electrospray ionization tandem mass spectrometry, Biology (General), PE, ethanolamine glycerophospholipid, Myeloperoxidase, TLC, thin layer chromatography, Organic Chemistry, FA, fatty acid, GC/MS, gas chromatography mass spectrometry, 2-ClFALD, 2-chlorofatty aldehyde, FALD, fatty aldehyde, Research Paper, Neutrophil activation

الوصف: Plasmalogens are a class of phospholipids containing vinyl ether linked aliphatic groups at the sn-1 position. Plasmalogens are known to contain 16- and 18-carbon aliphatic groups at the sn-1 position. Here, we reveal that the human neutrophil plasmenylethanolamine pool uniquely includes molecular species with very long carbon chain (VLC) aliphatic groups, including 20-, 22- and 24-carbon vinyl ether linked aliphatic groups at the sn-1 position. We identified these novel VLC plasmalogen species by electrospray ionization mass spectrometry methods. VLC plasmalogens were only found in the neutrophil plasmenylethanolamine pool. During neutrophil activation, VLC plasmenylethanolamines undergo myeloperoxidase-dependent oxidation to produce VLC 2-chlorofatty aldehyde and its oxidation product, 2-chlorofatty acid (2-ClFA). Furthermore, plasma concentrations of VLC 2-ClFA are elevated in human sepsis. These studies demonstrate for the first time VLC plasmenylethanolamine molecular species, their myeloperoxidase-mediated chlorolipid products and the presence of these chlorolipids in human sepsis.
Graphical abstract Image 1
Highlights • Novel very long chain (VLC) plasmenylethanolamine molecular species are discovered in human neutrophils. • VLC plasmenylethanolamines undergo myeloperoxidase dependent oxidation to produce new VLC chlorinated lipids. • VLC 2-chlorofatty acids are elevated in human sepsis.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::991be0461f8ad91bb141668ad24ed6d9Test
https://pubmed.ncbi.nlm.nih.gov/34902676Test

المؤلفون: Sena Özbay, U. Tansel Şireli

المساهمون: Rektörlük

المصدر: J Food Sci Technol

مصطلحات موضوعية: Meat Products, chemistry.chemical_compound, GC– MS (Gas chromatography–mass spectrometry), Risk groups, chemistry, N-nitrosamine, Nitrosamine, Sausage, Original Article, Food science, Ascorbic acid, Food Science

الوصف: This study aims to determine the amounts of seven volatile N-nitrosamine (VNA) derivatives which are in the risk group, in processed sausages. It also aimed to investigate the effects of the amount of added ascorbic acid, on the VNA level during the sausage manufacturing processes. For this purpose, meat doughs were prepared with two different levels of ascorbic acid. These meat doughs were put into the production process and packaged in two different packages. Thus, VNA derivatives and their amounts were determined according to production stages (heat treatments), packaging method (vacuum, MAP), storage process (Day 1, 7, 15, 30, 60, 90). As a result, it was found that the sausage product carries risk of VNA formation from the beginning of its production until the last day of storage before consumption for up to 90 days.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31137375236eb8be4bbd81cb7f184b38Test
https://pubmed.ncbi.nlm.nih.gov/35531396Test

المؤلفون: Lu, Ping-Hsun, Tseng, Chien-Wei, Lee, Jing-Ling, Lee, En-Yu, Lin, Yu-Ping, Lin, I-Hsin, Yu, Min-Chien, Lu, Kuo-Cheng, Kuo, Ko-Lin

المصدر: Pharmacological Research-Modern Chinese Medicine

مصطلحات موضوعية: ALI, acute lung injury, PrsDMe, prosapogenin D methyl ester, ASC, C-terminal caspase recruitment domain, AT1R, angiotensin II type I receptor, RNP, ribonucleoprotein, CCR, CC chemokine receptor, MPO, myeloperoxidase, Review Article, GC-MS, gas chromatography-mass spectrometry, Nrf2, nuclear factor erythroid-related factor 2, PLE, perilla leaf extract, AKT1, AKT serine/threonine kinase 1, CoVs, coronaviruses, TNF-α, tumor necrosis factor-α, GSK3β, glycogen synthase kinase beta, HIV-1, human immunodeficiency virus type 1, VCAM-1, vascular cell adhesion molecule 1, PRRs, pattern recognition receptors, ADRP, ADP ribose phosphatase, BALF, bronchoalveolar lavage fluid, ILC3, type 3 innate lymphoid cells, DIC, disseminated intravascular coagulation, RCT, randomized controlled trial, JAK2, Janus kinase 2, NF-κB, nuclear factor kappa B, ICAM-1, intercellular adhesion molecule 1, antivirus, PLpro, papain-like protease, Jing Si Herbal Drink, NO, nitrogen monoxide, RBD, receptor-binding domain, VEGF, vascular endothelial growth factor, STAT, signal transducer and activator of transcription, MMP-9, matrix metallopeptidase 9, Ang-II, angiotensin-II, LC–MS, liquid chromatography-mass spectrometry, COX-2, cyclooxygenase-2, iNOS, inducible nitric oxide synthase, LPS, lipopolysaccharide, TCM, traditional Chinese medicine, GM-CSF, granulocyte-macrophage colony-stimulating factor, NK, natural killer, PI3K, phosphoinositide 3-kinase, CPE, cytopathogenic effect, TLR, Toll-like receptor, ATF2, activating transcription factor 2, NETs, neutrophil extracellular traps, RA, rosmarinic acid, JSHD, Jing Si herbal drink, NEK7, NIMA-related kinase 7, RdRP, RNA-dependent RNA polymerase, HLH, hemophagocytic lymphohistiocytosis, mechanism, ACE2, angiotensin-converting enzyme 2, complex mixtures, NLRP3, NLR family pyrin domain-containing 3, WHO, World Health Organization, ROS, reactive oxygen species, 3CLpro, 3-chymotrypsin-like cysteine protease, SOD, superoxide dismutase, TGF-β, transforming growth factor-β, RORγ, RAR-related orphan receptor γ, NE, neutrophil elastase, PD, platycodin D, IFN, interferon, CSFR, colony-stimulating factor receptor, TXA2, thromboxane A2, VOC, variants of concern, ARDS, acute respiratory distress syndrome, citH3, citrullinated H3, PG, Platycodon grandiflorum, COVID-19, herbs, DA, dehydromatricarin A, HSP70, heat shock protein 70, SM, Seomae mugwort, anti-inflammation, HIF-1, hypoxia-inducible factor 1, HMGB1, high mobility group box 1, AA, Artemisia argyi, Mpro, main protease, COPD, chronic obstructive pulmonary disease, CSF, colony-stimulating factor, DAMPs, damage-associated molecular patterns, Nsps, nonstructural proteins, TMPRSS2, transmembrane serine protease 2, MAPK, mitogen-activated protein kinase

الوصف: Background : SARS-CoV-2 has led to a sharp increase in the number of hospitalizations and deaths from pneumonia and multiorgan disease worldwide; therefore, SARS-CoV-2 has become a global health problem. Supportive therapies remain the mainstay treatments against COVID-19, such as oxygen inhalation, antiviral drugs, and antibiotics. Traditional Chinese medicine (TCM) has been shown clinically to relieve the symptoms of COVID-19 infection, and TCMs can affect the pathogenesis of SARS-CoV-2 infection in vitro. Jing Si Herbal Drink (JSHD), an eight herb formula jointly developed by Tzu Chi University and Tzu Chi Hospital, has shown potential as an adjuvant treatment for COVID-19 infection. A randomized controlled trial (RCT) of JSHD as an adjuvant treatment in patients with COVID-19 infection is underway Objectives : This article aims to explore the efficacy of the herbs in JSHD against COVID-19 infection from a mechanistic standpoint and provide a reference for the rational utilization of JSHD in the treatment of COVID-19. Method : We compiled evidence of the herbs in JSHD to treat COVID-19 in vivo and in vitro. Results : We described the efficacy and mechanism of action of the active ingredients in JSHD to treat COVID-19 based on experimental evidence. JSHD includes 5 antiviral herbs, 7 antioxidant herbs, and 7 anti-inflammatory herbs. In addition, 2 herbs inhibit the overactive immune system, 1 herb reduces cell apoptosis, and 1 herb possesses antithrombotic ability. Conclusion : Although experimental data have confirmed that the ingredients in JSHD are effective against COVID-19, more rigorously designed studies are required to confirm the efficacy and safety of JSHD as a COVID-19 treatment.
Graphical Abstract Image, graphical abstract

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2244e4d95e5172a8359236fa899ba577Test
https://doi.org/10.1016/j.prmcm.2021.100024Test

المؤلفون: Weiqi Li, Yanxia Jia

المصدر: Plant Diversity
Plant Diversity, Vol 40, Iss 6, Pp 292-298 (2018)

مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, GC-MS, gas chromatography-mass spectrometry, Plant Science, DMSO, dimethyl sulfoxide, PG, phosphatidylglycerol, 01 natural sciences, chemistry.chemical_compound, lcsh:Botany, Arabidopsis, MGDG, monogalactosyldiacylglycerol, Radicle, lcsh:QH301-705.5, chemistry.chemical_classification, biology, food and beverages, Phosphatidic acid, lcsh:QK1-989, Biochemistry, Germination, lipids (amino acids, peptides, and proteins), 1-butanol, Cotyledon, TAG, triacylglycerols, PC, Phosphatidylcholine, food.ingredient, NAE, N-Acylethanolamines, N-Acylethanolamines, PE, phosphatidylethanolamine, Article, PI, phosphatidylinositol, 03 medical and health sciences, food, PLD, phospholipase D, Ecology, Evolution, Behavior and Systematics, Phospholipase D, organic chemicals, FA, fatty acid, lysoPC, lysophosphatidylcholine, DGDG, digalactosyldiacylglycerol, Fatty acid, biology.organism_classification, 030104 developmental biology, lcsh:Biology (General), chemistry, Seedling, Triacylglycerols, 010606 plant biology & botany

الوصف: Storage oil breakdown plays an important role in the life cycle of many plants by providing the carbon skeletons that support seedling growth immediately following germination. 1-Butanol, a specific inhibitor of phospholipase D (PLD)-dependent production of the signalling molecule phosphatidic acid (PA), inhibited Arabidopsis seed germination. N-Acylethanolamines (NAEs), which have been shown to inhibits PLDα1 activity, have no effect on seed germination. However, mobilization profile of triacylglycerols (TAG) that induced by each compound has not been reported. To gain deeper insights into the mode of mobilization of TAG during NAE 12:0 or 1-butanol treatment, we conducted a detailed comparative analysis of the effect of NAE 12:0, DMSO, 1-butanol and tert-butanol on Arabidopsis seed germination and fatty acid composition, tert-butanol and DMSO served as the corresponding controls treatment respectively. Our data show that 1-butanol, but not the inactive tert-butanol isomer, inhibited Arabidopsis seed germination, which is accompanied by a with retardation of the mobilization of triacylglycerols (TAG). In contrast, NAE 12:0 did not affect mobilization of TAG, nor did it significantly delay seed germination as monitored by radicle and cotyledon emergence. 1-Butanol induced RNA degradation in seeds and seedlings. We speculate that the large-scale degradation of RNA under the induction of 1-butanol may lead to abnormal gene expression in genes necessary for seed germination, including the genes needed for the mobilization of oil bodies, and thus cause a delay of seed germination. To the best of our knowledge, we report for the first time that 1-butanol delays the mobilization of TAG. Keywords: Germination, 1-butanol, N-Acylethanolamines, Triacylglycerols, Fatty acid

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::646d7bd17adbc61c14b64ff496e8d867Test
https://doi.org/10.1016/j.pld.2018.11.002Test

المؤلفون: Angelika Schoster, Déborah Mathis, Jörn Oliver Sass, Claudia Graubner

المساهمون: University of Zurich, Schoster, Angelika

المصدر: Mathis, Déborah; Sass, Jörn Oliver; Graubner, Claudia; Schoster, Angelika (2021). Diagnosis of atypical myopathy based on organic acid and acylcarnitine profiles and evolution of biomarkers in surviving horses. Molecular genetics and metabolism reports, 29, p. 100827. Elsevier 10.1016/j.ymgmr.2021.100827 <http://dx.doi.org/10.1016/j.ymgmr.2021.100827Test>
Molecular Genetics and Metabolism Reports, Vol 29, Iss, Pp 100827-(2021)
Molecular Genetics and Metabolism Reports
Molecular Genetics and Metabolism Reports 29 (2021) 100827

مصطلحات موضوعية: Pathology, medicine.medical_specialty, Medicine (General), QH301-705.5, Acquired multiple acyl-CoA dehydrogenase deficiency, AC, acylcarnitines, MADD, Multiple acyl-CoA dehydrogenase deficiency, 610 Medicine & health, R5-920, Endocrinology, 1311 Genetics, 1312 Molecular Biology, Genetics, Medicine, ddc:576, Biology (General), Myopathy, Molecular Biology, chemistry.chemical_classification, 630 Agriculture, business.industry, AM, Atypical myopathy, Hypoglycin A, 1310 Endocrinology, DBS, dried blot spots, OA, organic acids, chemistry, 10036 Medical Clinic, Seasonal pasture myopathy, 570 Life sciences, biology, 10090 Equine Department, medicine.symptom, GC–MS, gas chromatography–mass spectrometry, business, Organic acid, Research Paper

الوصف: Background Atypical myopathy (AM), an acquired multiple acyl-CoA dehydrogenase deficiency (MADD) in horses, induce changes in mitochondrial metabolism. Only few veterinary laboratories offer diagnostic testing for this disease. Inborn and acquired MADD exist in humans, therefore determination of organic acids (OA) in urine and acylcarnitines (AC) in blood by assays available in medical laboratories can serve as AM diagnostics. The evolution of OA and AC profiles in surviving horses is unreported. Methods AC profiles using electrospray ionization tandem mass spectrometry (ESI-MS/MS) and OA in urine using gas chromatography mass spectrometry (GC–MS) were determined in dried blot spots (DBS, n = 7) and urine samples (n = 5) of horses with AM (n = 7) at disease presentation and in longitudinal samples from 3/4 survivors and compared to DBS (n = 16) and urine samples (n = 7) from control horses using the Wilcoxon test. Results All short- (C2-C5) and medium-chain (C6-C12) AC in blood differed significantly (p
Highlights • Human medical laboratories can be used to diagnose atypical myopathy in horses. • Diagnosis can be achieved by organic acid and acylcarnitine profiling. • Diagnosis can also be achieved in convalescent horses. • Specific metabolites are still evident several days after clinical signs start. • Some metabolites differentiated between survivors and non-survivors.

وصف الملف: Mathis_2021_MGM_Reports_AM_diagnosis_with_human_methods.pdf - application/pdf; application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::608f5880587b0050230bd71125d2871dTest

المؤلفون: Alfred Ngenge, Tamfu, Ozgur, Ceylan, Selcuk, Kucukaydin, Mehmet, Ozturk, Mehmet Emin, Duru, Rodica Mihaela, Dinica

المصدر: Foods

مصطلحات موضوعية: violacein inhibition, swimming inhibition, antibiofilm, parasitic diseases, GC-MS (gas chromatography mass spectrometry), antimicrobial, swarming inhibition, anticholinesterase, African food spices, antiurease, Article, antityrosinase

الوصف: Food pathogens represent an important health threat, and it is relevant to study the effect of foodstuffs such as spices which can inhibit bacterial growth. This study reports the antimicrobial, antibiofilm, and enzyme (Acetylcholinesterase, Butyrylcholinesterase, urease, tyrosinase) inhibitory activities of two medicinal food spices belonging to the Annonaceae family, Monodora myristica and Xylopia aethiopica. GC-MS (gas chromatography mass spectrometry) analysis of silylated samples of Methanol-Dicloromethane (50:50) extracts of both plants led to the identification of nine compounds in M. myristica and seven compounds in X. aethiopica. M. myristica and X. aethiopica had the same minimum inhibitory concentration (MIC) values of 0.625 mg/mL and 2.5 mg/mL on C. albicans and E. coli, respectively. However, M. myristica had better activity than X. aethiopica on Staphylococcus aureus, while Pseudomonas aeruginosa was more susceptible to X. aethiopica than M. myristica. The lowest MIC value was 0.1325 mg/mL, exhibited by M. myristica on S. aureus. Both extracts showed good antibiofilm activity. On S. aureus, at the same concentration, M. myristica had better antibiofilm activity than X. aethiopica. On E. coli and Candida albicans, X. aethiopica had better antibiofilm activity than M. myristica at the same concentration. X. aethiopica showed better violacein inhibition in Chromobacterium violaceum CV12472, as its percentage inhibition of violacein varied from 80.5% ± 3.0% at MIC to 5.6 ± 0.2 at MIC/8, as compared to M. myristica with 75.1% ± 2.5% at MIC and 15.5% ± 1.1% at MIC/8. The anti-motility activity by swimming and swarming inhibition on P. aeruginosa PA01 was low at test concentrations and in both models, M. myristica showed higher motility inhibition than X. aethiopica. Although in enzyme inhibitory assays all extracts had low inhibitions compared to standards tested at the same concentrations, the results show that these plants can be used to manage food-borne infections.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::6f752f861809bcaf902dd14eb15153c1Test
https://pubmed.ncbi.nlm.nih.gov/33260317Test

المؤلفون: Christoph Peschel, Fabian Horsthemke, Martin Winter, Sascha Nowak

المصدر: MethodsX
MethodsX, Vol 9, Iss, Pp 101621-(2022)

مصطلحات موضوعية: Science, GC-HRMS, Clinical Biochemistry, EI, electron ionization, Target analysis, BMC, n-butyl methyl carbonate, sBEC, sec-butyl ethyl carbonate, Method Article, LIB, lithium ion battery, Orbitrap mass spectrometry, EPC, ethyl propyl carbonate, BEC, n-butyl ethyl carbonate, MPC, methyl propyl carbonate, SQ, single quadrupole, Lithium ion battery electrolytes, EIC, extracted ion chromatogram, Target analysis via GC-HRMS of lithium ion battery electrolyte decomposition, HRMS, high resolution mass spectrometry, Medical Laboratory Technology, GC-MS, gas chromatography – mass spectrometry, FWHM, full width at half maximum, sBMC, sec-butyl methyl carbonate, SPME, solid phase microextraction

الوصف: The implementation of orbitrap mass spectrometry for target analysis of volatile species in aged lithium-ion batteries was performed in a case study on butyl carbonates. In comparison to previously applied single quadrupole-based methods, major improvements were obtained.•Sensitivity was improved by effectively background free extracted ion chromatograms of identified marker fragment ions.•Typical isobaric interferences of typical carbonate fragment ions e.g. caused by column bleeding were identified and false positive identification avoided.•Analysis of isotope labeled electrolytes was optimized regarding mass spectrometric data reliability with mass accuracies 100,000.
Graphical abstract Image, graphical abstract

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::36afaecbddb047bb37f6f9acd8e58a5dTest
https://doi.org/10.1016/j.mex.2022.101621Test