Zfp57 inactivation illustrates the role of ICR methylation in imprinted gene expression during neural differentiation of mouse ESCs
| العنوان: | Zfp57 inactivation illustrates the role of ICR methylation in imprinted gene expression during neural differentiation of mouse ESCs |
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| المؤلفون: | Claudia Angelini, Ankit Verma, Basilia Acurzio, Carlo Giaccari, Floriana Della Ragione, Francesco Cecere, Andrea Riccio, Robert Feil, Flavia Cerrato, Annalisa Fico, Alessia Polito, Salvatore Fioriniello |
| المساهمون: | Acurzio, B., Verma, A., Polito, A., Giaccari, C., Cecere, F., Fioriniello, S., Della Ragione, F., Fico, A., Cerrato, F., Angelini, C., Feil, R., Riccio, A., Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) |
| المصدر: | Scientific reports (Nature Publishing Group) 11 (2021). doi:10.1038/s41598-021-93297-3 info:cnr-pdr/source/autori:Acurzio B.; Verma A.; Polito A.; Giaccari C.; Cecere F.; Fioriniello S.; Della Ragione F.; Fico A.; Cerrato F.; Angelini C.; Feil R.; Riccio A./titolo:Zfp57 inactivation illustrates the role of ICR methylation in imprinted gene expression during neural differentiation of mouse ESCs/doi:10.1038%2Fs41598-021-93297-3/rivista:Scientific reports (Nature Publishing Group)/anno:2021/pagina_da:/pagina_a:/intervallo_pagine:/volume:11 Scientific Reports Scientific Reports, Nature Publishing Group, 2021, 11 (1), ⟨10.1038/s41598-021-93297-3⟩ Scientific Reports, Vol 11, Iss 1, Pp 1-17 (2021) |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | nervous system development, wildtype, Mutant, Zfp57, Mice, 0302 clinical medicine, Neural Stem Cells, Neural Stem Cell, Imprinting (psychology), cell mutant, [SDV.BDD]Life Sciences [q-bio]/Development Biology, Regulation of gene expression, 0303 health sciences, Multidisciplinary, allele, article, Gene Expression Regulation, Developmental, Mouse Embryonic Stem Cells, Imprinting, Cell Differentiation, Methylation, protein function, ORIGIN-SPECIFIC EXPRESSIONDNA METHYLATIONDISTAL CHROMOSOME-7STEM-CELLSIDENTIFICATIONMULTIPLEMECHANISMSCHROMATINMAINTAINSTRANSIENT, Cell biology, DNA methylation, Medicine, Epigenetics, Science, Biology, Chromosome, Chromosomes, Genomic Imprinting, 03 medical and health sciences, hybrid mouse strain, Genetics, Animals, controlled study, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Gene, 030304 developmental biology, cell culture, [SDV.GEN]Life Sciences [q-bio]/Genetics, ES-derived neural progenitor cells, Animal, Mouse Embryonic Stem Cell, DNA Methylation, Repressor Proteins, JB1, methylation, Genomic imprinting, Zfp57, Genetics, Epigenetics, Imprinting, allele, article, cell culture, cell mutant, chromosome, controlled study, hybrid mouse strain, methylation, mouse embryonic stem cell, nervous system development, protein function, ES-derived neural progenitor cells, JB1, wildtype, Zfp57, 030217 neurology & neurosurgery |
| الوصف: | ZFP57 is required to maintain the germline-marked differential methylation at imprinting control regions (ICRs) in mouse embryonic stem cells (ESCs). Although DNA methylation has a key role in genomic imprinting, several imprinted genes are controlled by different mechanisms, and a comprehensive study of the relationship between DMR methylation and imprinted gene expression is lacking. To address the latter issue, we differentiated wild-type and Zfp57-/- hybrid mouse ESCs into neural precursor cells (NPCs) and evaluated allelic expression of imprinted genes. In mutant NPCs, we observed a reduction of allelic bias of all the 32 genes that were imprinted in wild-type cells, demonstrating that ZFP57-dependent methylation is required for maintaining or acquiring imprinted gene expression during differentiation. Analysis of expression levels showed that imprinted genes expressed from the non-methylated chromosome were generally up-regulated, and those expressed from the methylated chromosome were down-regulated in mutant cells. However, expression levels of several imprinted genes acquiring biallelic expression were not affected, suggesting the existence of compensatory mechanisms that control their RNA level. Since neural differentiation was partially impaired in Zfp57-mutant cells, this study also indicates that imprinted genes and/or non-imprinted ZFP57-target genes are required for proper neurogenesis in cultured ESCs. |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04bb9c4281c514697366597f30862f67Test https://doi.org/10.1038/s41598-021-93297-3Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....04bb9c4281c514697366597f30862f67 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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