Spindle-F Is the Central Mediator of Ik2 Kinase-Dependent Dendrite Pruning in Drosophila Sensory Neurons
| العنوان: | Spindle-F Is the Central Mediator of Ik2 Kinase-Dependent Dendrite Pruning in Drosophila Sensory Neurons |
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| المؤلفون: | Hsu-Chen Cheng, Kai-Wen Chiang, Hsin-Lun Hsieh, Chiou-Yang Tang, Yu-Ting Lai, Tzu Lin, June-Tai Wu, Shi-Bing Yang, Shih-Sian Liao, Po-Yuan Pan, Yuh Nung Jan, Yi-Ping Wu, Hsiu-Hsiang Lee, Jian-Ming Ke, Hsueh-Tzu Shih, Myong-Chol Lee |
| المصدر: | PLoS Genetics, Vol 11, Iss 11, p e1005642 (2015) PLoS Genetics |
| بيانات النشر: | Public Library of Science (PLoS), 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Cytoplasm, Cancer Research, Sensory Receptor Cells, lcsh:QH426-470, Dynein, Sensory system, Dendrite, Microtubule, Genetics, medicine, Animals, Drosophila Proteins, Phosphorylation, Molecular Biology, Genetics (clinical), Ecology, Evolution, Behavior and Systematics, biology, fungi, I-Kappa-B Kinase, Dyneins, Dendrites, Anatomy, biology.organism_classification, I-kappa B Kinase, Cell biology, lcsh:Genetics, Drosophila melanogaster, medicine.anatomical_structure, nervous system, Drosophila Protein, Research Article |
| الوصف: | During development, certain Drosophila sensory neurons undergo dendrite pruning that selectively eliminates their dendrites but leaves the axons intact. How these neurons regulate pruning activity in the dendrites remains unknown. Here, we identify a coiled-coil protein Spindle-F (Spn-F) that is required for dendrite pruning in Drosophila sensory neurons. Spn-F acts downstream of IKK-related kinase Ik2 in the same pathway for dendrite pruning. Spn-F exhibits a punctate pattern in larval neurons, whereas these Spn-F puncta become redistributed in pupal neurons, a step that is essential for dendrite pruning. The redistribution of Spn-F from puncta in pupal neurons requires the phosphorylation of Spn-F by Ik2 kinase to decrease Spn-F self-association, and depends on the function of microtubule motor dynein complex. Spn-F is a key component to link Ik2 kinase to dynein motor complex, and the formation of Ik2/Spn-F/dynein complex is critical for Spn-F redistribution and for dendrite pruning. Our findings reveal a novel regulatory mechanism for dendrite pruning achieved by temporal activation of Ik2 kinase and dynein-mediated redistribution of Ik2/Spn-F complex in neurons. Author Summary In Drosophila, the nervous systems undergo extensive neuronal remodeling during metamorphosis, as many larval neurons die and adult neurons are generated while some larval neurons survive and prune their branches. Pruning that removes specific parts of neuronal branches without causing cell death is a self-destruct process, thus requiring precise regulation to prevent undesired damage to the nervous systems. Certain Drosophila sensory neurons that undergo dendrite pruning, specifically eliminating the dendrites but leaving the axons intact, provide us an opportunity to study the mechanism of how pruning activity is regulated in the dendrites. We reasoned that the distinctive microtubule polarity in dendrites and axons might be involved and factors are required to regulate the pruning activity in the dendrites through their interaction with microtubules. Here, we identified Spindle-F that mediates Ik2-dependent pruning activity in the dendrites by linking Ik2 to the microtubule motor dynein complex. We showed that elevation of Ik2 activity during dendrite pruning promotes Ik2/Spindle-F/dynein complex moving along the microtubules. We also showed that the formation and redistribution of Ik2/Spindle-F/dynein complex are essential for dendrite pruning. Our study reveals a connection between the polarized microtubules of dendrites and the pruning activity through Spindle-F for dendrite pruning. |
| تدمد: | 1553-7404 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::182eed586f78c3dd0bda3f26736ccb44Test https://doi.org/10.1371/journal.pgen.1005642Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....182eed586f78c3dd0bda3f26736ccb44 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15537404 |
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