Single-Nucleus Chromatin Accessibility Landscape Reveals Diversity in Regulatory Regions Across Distinct Adult Rat Cortex
| العنوان: | Single-Nucleus Chromatin Accessibility Landscape Reveals Diversity in Regulatory Regions Across Distinct Adult Rat Cortex |
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| المؤلفون: | Yeya Yu, Xiaoyu Wei, Qiuting Deng, Qing Lan, Yiping Guo, Lei Han, Yue Yuan, Peng Fan, Peiying Wu, Shuncheng Shangguan, Yang Liu, Yiwei Lai, Giacomo Volpe, Miguel A. Esteban, Chuanyu Liu, Yong Hou, Longqi Liu |
| المصدر: | Frontiers in Molecular Neuroscience Frontiers in Molecular Neuroscience, Vol 14 (2021) |
| بيانات النشر: | Frontiers Media SA, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, snATAC-seq, Cell type, Neurosciences. Biological psychiatry. Neuropsychiatry, Biology, Cell fate determination, 03 medical and health sciences, Cellular and Molecular Neuroscience, 0302 clinical medicine, Cortex (anatomy), Data Report, medicine, Molecular Biology, Gene, Transcription factor, transcription factor, regulatory element, Chromatin, 030104 developmental biology, medicine.anatomical_structure, chromatin accessibility, Regulatory sequence, rat cerebral cortex, Molecular Neuroscience, Neuroscience, Nucleus, 030217 neurology & neurosurgery, RC321-571 |
| الوصف: | Rats have been widely used as an experimental organism in psychological, pharmacological, and behavioral studies by modeling human diseases such as neurological disorders. It is critical to identify and characterize cell fate determinants and their regulatory mechanisms in single-cell resolutions across rat brain regions. Here, we applied droplet-based single-nucleus assay for transposase-accessible chromatin using sequencing (snATAC-seq) to systematically profile the single-cell chromatin accessibility across four dissected brain areas in adult Sprague–Dawley (SD) rats with a total of 59,023 single nuclei and identified 16 distinct cell types. Interestingly, we found that different cortex regions exhibit diversity in both cellular compositions and gene regulatory regions. Several cell-type-specific transcription factors (TFs), including SPI1, KLF4, KLF6, and NEUROD2, have been shown to play important roles during the pathogenesis of various neurological diseases, such as Alzheimer’s disease (AD), astrocytic gliomas, autism spectrum disorder (ASD), and intellectual disabilities. Therefore, our single-nucleus atlas of rat cortex could serve as an invaluable resource for dissecting the regulatory mechanisms underlying diverse cortex cell fates and further revealing the regulatory networks of neuropathogenesis. |
| تدمد: | 1662-5099 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f7e806d86e2ad00e70be9475c9f3742fTest https://doi.org/10.3389/fnmol.2021.651355Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f7e806d86e2ad00e70be9475c9f3742f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16625099 |
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