Coordination of Differential Effects of Primary Estrogen and Catecholestrogen on Two Distinct Targets Mediates Embryo Implantation in the Mouse**This work was supported, in part, by NIH Grant HD-12304 and as part of the National Cooperative Program on Markers of Uterine Receptivity for Blastocyst Implantation [NIH Grants HD-29968 (to S.K.D.), HD-35114 (to B.C.P.), and ES-07814 (to S.K.D.)]. A center grant in Reproductive Biology (HD-33994) and a center grant in Mental Retardation and Developmental Disabilities (HD-02528) provided access to various core facilities
| العنوان: | Coordination of Differential Effects of Primary Estrogen and Catecholestrogen on Two Distinct Targets Mediates Embryo Implantation in the Mouse**This work was supported, in part, by NIH Grant HD-12304 and as part of the National Cooperative Program on Markers of Uterine Receptivity for Blastocyst Implantation [NIH Grants HD-29968 (to S.K.D.), HD-35114 (to B.C.P.), and ES-07814 (to S.K.D.)]. A center grant in Reproductive Biology (HD-33994) and a center grant in Mental Retardation and Developmental Disabilities (HD-02528) provided access to various core facilities |
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| المؤلفون: | Hyunjung Jade Lim, Joachim G. Liehr, Xiao-Ning Wang, Sudhansu K. Dey, Sanjoy K. Das, Bibhash C. Paria |
| المصدر: | Endocrinology. 139:5235-5246 |
| بيانات النشر: | The Endocrine Society, 1998. |
| سنة النشر: | 1998 |
| مصطلحات موضوعية: | medicine.medical_specialty, urogenital system, medicine.drug_class, Blastocyst Transfer, Uterus, Estrogen receptor, Biology, Antiestrogen, Paracrine signalling, Endocrinology, medicine.anatomical_structure, Estrogen, Internal medicine, medicine, Blastocyst, Protein kinase A |
| الوصف: | In the mouse, estrogen is essential for blastocyst implantation in the progesterone (P4)-primed uterus. The mechanism(s) by which estrogen initiates this response still remains elusive. The present investigation, using delayed implantation in the mouse, examined the differential role of estradiol-17beta (E2) and its catechol metabolite 4-hydroxy-E2 (4-OH-E2) in uterine and blastocyst activation for implantation. The conditions of delayed implantation were induced by ovariectomizing mice on day 4 (day 1 = vaginal plug) of pregnancy or pseudopregnancy and maintaining them with P4 from days 5-7. The binding of EGF to blastocysts was used as a marker for blastocyst activation. Our results show that whereas E2 fails to activate dormant blastocysts (with respect to EGF binding in vitro), 4-OH-E2, cAMP, or prostaglandin E2, is effective in this response. Further, whereas 4-OH-E2 induced-activation is not blocked by an antiestrogen, an inhibitor of PG synthesis, adenylyl cyclase or protein kinase A effectively blocks this activation. These results suggest that 4-OH-E2 effects on blastocysts are mediated by PGs, which, in turn, stimulate cAMP production and thus activation of protein kinase A. Two-fluoro-E2 is a poor substrate and an inhibitor of catecholestrogen synthesis, but it is estrogenic, with respect to uterine growth and gene expression. Using blastocyst transfer experiments, we observed that dormant blastocysts incubated with 4-OH-E2 in vitro, but not with E2, are capable of implanting in P4-treated delayed implanting mice receiving two-fluoro-E2. The results suggest that whereas E2 is necessary for preparation of the uterus, uterine-derived catecholestrogen is important for blastocyst activation for implantation. Indeed, the receptive uterus has the capacity to synthesize 4-OH-E2. Collectively, we demonstrate that the primary ovarian estrogen E2, via its interaction with nuclear estrogen receptors, participates in the preparation of the P4-primed uterus to the receptive state in an endocrine manner, whereas its metabolite 4-OH-E2, produced from E2 in the uterus, mediates blastocyst activation for implantation in a paracrine manner. Our results also establish that these target-specific effects of primary estrogen and catecholestrogen are both essential for implantation and that successful implantation occurs only when the activated stage of the blastocyst coincides with the receptive state of the uterus. |
| تدمد: | 1945-7170 0013-7227 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::18318b05dae59d7df14322b21a47fd8cTest https://doi.org/10.1210/endo.139.12.6386Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........18318b05dae59d7df14322b21a47fd8c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19457170 00137227 |
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