Modulation of equol production via different dietary regimens in an artificial model of the human colon
| العنوان: | Modulation of equol production via different dietary regimens in an artificial model of the human colon |
|---|---|
| المؤلفون: | Lucía Vázquez, Baltasar Mayo, Ana Belén Flórez, Begoña Redruello, Koen Venema, Sanne Verbruggen, Jessica Verhoeven |
| المساهمون: | Ministerio de Economía y Competitividad (España), Principado de Asturias, Maastricht University, Venema, Koen [0000-0001-7046-5127], Mayo Pérez, Baltasar [0000-0001-5634-6543], Venema, Koen, Mayo Pérez, Baltasar |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname Journal of Functional Foods, Vol 66, Iss, Pp 103819-(2020) |
| بيانات النشر: | Elsevier, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Intestinal microbiota, TIM-2, Medicine (miscellaneous), Endogeny, Butyrate, Biology, 03 medical and health sciences, chemistry.chemical_compound, 0404 agricultural biotechnology, Biosynthesis, TX341-641, Food science, Human faeces, 030109 nutrition & dietetics, Nutrition and Dietetics, Nutrition. Foods and food supply, Daidzein, Intestinal model, food and beverages, Soy isoflavones, 04 agricultural and veterinary sciences, Equol, Carbohydrate, Isoflavones, biology.organism_classification, 040401 food science, chemistry, Bacteria, Food Science |
| الوصف: | In order to find dietary conditions favouring endogenous equol biosynthesis, a pooled faecal homogenate from equol-producing women was used to inoculate the TIM-2 artificial model of the human proximal colon. The model was fuelled with control diets not supplemented (C) or supplemented (C-ISO) with isoflavones, and two isoflavone-containing diets rich in carbohydrate (CH-ISO) or protein (PR-ISO). Compared to the C-ISO control, the CH-ISO diet doubled the production of equol, while with the PR-ISO diet the production of equol in cultures decreased sharply. The CH-ISO diet was also associated with enhanced butyrate production. The numbers of most bacterial populations analysed did not significantly change along cultures with any of the diets. Surprisingly, counts for a gene involved in equol production (tdr) were reduced in all cultures, reflecting a reduction in the number of equol-producing bacteria. In conclusion, under the TIM-2 culture conditions established, the CH-ISO diet favoured the synthesis of equol. This study was partly supported by projects from the Spanish Ministry of Economy and Competitiveness (MINECO) (AGL-2014-57820-R) and Asturias Principality (IDI/2018/000114). LV was supported by a contract from MINECO within the FPI Program (BES-2015-072285). In addition, the study was also funded by the Centre for Healthy Eating & Food Innovation (HEFI) of Maastricht University, Campus Venlo. This research has been made possible with the support of the Dutch Province of Limburg. |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::839eb40f29879c629ef86f0db850dc64Test http://hdl.handle.net/10261/223094Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....839eb40f29879c629ef86f0db850dc64 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |