Absolute Stereochemistry of Fungal Beauveriolide III and ACAT Inhibitory Activity of Four Stereoisomers
| العنوان: | Absolute Stereochemistry of Fungal Beauveriolide III and ACAT Inhibitory Activity of Four Stereoisomers |
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| المؤلفون: | Takayuki Doi, Taichi Ohshiro, Takafumi Sekiguchi, Kenichiro Nagai, Lawrence L. Rudel, Kazuaki Akasaka, Ichiji Namatame, Takashi Takahashi, Hiroshi Tomoda, Satoshi Omura |
| المصدر: | The Journal of Organic Chemistry. 71:9252-9252 |
| بيانات النشر: | American Chemical Society (ACS), 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Ketone, Stereochemistry, Sterol O-acyltransferase, Beauveriolide III, Inhibitory postsynaptic potential, Chemical synthesis, Isozyme, Structure-Activity Relationship, Depsipeptides, Animals, Humans, Organic chemistry, chemistry.chemical_classification, Depsipeptide, Chemistry, Macrophages, Organic Chemistry, Fungi, Absolute configuration, Stereoisomerism, Lipid Metabolism, Lipids, Cyclic peptide, Enzyme, Biochemistry, lipids (amino acids, peptides, and proteins), Sterol O-Acyltransferase |
| الوصف: | Fungal beauveriolide III (BeauIII, 1b), a cyclodepsipeptide inhibiting acyl-CoA:cholesterol acyltransferase (ACAT) and showing antiatherogenic activity in mouse models, consists of L-Phe, L-Ala, D-allo-Ile, and 3-hydroxy-4-methyloctanoic acid (HMA) moieties, but the stereochemistry of the HMA part has not until now been fully defined. To determine it, four HMA stereoisomers were synthesized and labeled with (S)-(+)-2-(anthracene-2,3-dicarboximido)-1-propyl trifluoromethane sulfonate (AP-OTf), a chiral fluorescent reagent. The derivatives were separated by HPLC and compared with the natural HMA derivative, which was thereby identified as (3S,4S)HMA in BeauIII. Furthermore, the four beauveriolide III isomers ((3S,4S)BeauIII (23a), (3R,4R)BeauIII (23b), (3R,4S)BeauIII (23c), and (3S,4R)BeauIII (23d)) were synthesized, and it was shown that all the spectral data for 23a were identical with those for natural 1b. Isomers 23a and 23d showed potent inhibitory activity of lipid droplet accumulation in macrophages, while the other two isomers caused weak inhibition. Thus, the 3S configuration of BeauIII is important for this activity. Furthermore, 23a and 23d showed rather specific inhibition against the ACAT1 isozyme. |
| تدمد: | 1520-6904 0022-3263 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63a62bb50616ee794f1a374b3093480aTest https://doi.org/10.1021/jo062058nTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....63a62bb50616ee794f1a374b3093480a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15206904 00223263 |
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